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Research Article Open access CC BY 3.0

Technological Properties of Lactococcus lactis subsp. lactis I23 Isolated from Nigerian Beef and Characterization of Bacteriocin Produced by It

O. A. Olaoye

Biotechnology Journal International · pp. 829–845 · Published 17 Jul 2014

10.9734/BBJ/2014/11719

Abstract

Technological Properties of Lactococcus lactis subsp. lactis I23 Isolated from Nigerian Beef and Characterization of Bacteriocin Produced by It Aim: The present study reports the phenotypic and molecular characterization of Lactococcus strains isolated from Nigerian beef. The technological properties of selected strains were evaluated in order to test their suitability as candidates of starter cultures for food preservation. Study Design: Experimental microbiological testing. Place and Duration of Study: Microbiology Department, University of Ibadan, Nigeria  and Division of Food Sciences, The University of Nottingham, UK; between January 2005 and February 2009. Methodology: Phenotypic method was adopted in the initial characterization of the presumptive Lactococcus strains which were later characterized using pulsed field gel electrophoresis. The use of 16S rDNA sequencing was adopted in the full identification of selected strains. High performance liquid chromatography and Gas chromatography were employed in determining production of organic acids and diacetyl respectively by the strains of Lactococcus. Testing and identification of bacteriocin production by the strains were observed using in vitro and polymerase chain reaction methods. The antimicrobial activities of the strains were evaluated against known spoilage and pathogenic organisms of food origin. Results: Three strains, Lc. lactis L11, Lc. lactis subsp. lactis I23 and Lc. lactis subsp. hordinae E91 showed better production of diacetyl and lactic acid than others; concentrations in excess of 31.15 µg/10-7cfu and 17.56 g/107cfu at 18 and 24 h of incubation were recorded for the respective compounds. Result of their antimicrobial activities indicates that varying levels of inhibition were recorded against the spoilage/pathogenic organisms; however no inhibition was recorded against Brochothrix thermosphacta except by Lc. lactis subsp lactis I23 which was shown to encode gene for production of bacteriocin, nisin. The nucleotide sequence of the bacteriocin was deposited at the genBank under accession number EU667387. Conclusion: The ability of the three Lactococcus strains to combat spoilage and pathogenic organisms could help promote safety in food processing and preservation in Nigeria; moreover, production of bacteriocin by Lc. lactis subsp. lactis I23 may confer added advantage during choice of candidacy for starter cultures in food biopreservation.

Antimicrobial agents spoilage and pathogenic organisms inhibition bacteriocin safety food biopreservation

References (25)

  1. 1 Lactic acid bacteria of foods and their current taxonomy [DOI]
  2. 2 Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production: An update [DOI]
  3. 3 Selection criteria for lactic acid bacteria to be used as starter cultures for various food commodities [DOI]
  4. 4 Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility [DOI]
  5. 5 Functional properties of novel protective lactic acid bacteria and application in raw chicken meat against Listeria monocytogenes and Salmonella enteritidis [DOI]
  6. 6 Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage [DOI]
  7. 7 Safety properties and molecular strain typing of lactic acid bacteria from slightly fermented sausages [DOI]
  8. 8 Characterization of two bacteriocins produced by Pediococcus acidilactici isolated from “Alheira”, a fermented sausage traditionally produced in Portugal [DOI]
  9. 9 Genera Leuconostoc, Oenococcus and Weissella [DOI]
  10. 10 Isolation of lactic acid bacteria with inhibitory activity against pathogens and spoilage organisms associated with fresh meat [DOI]
  11. 11 Behavior of Variable V3 Region from 16S rDNA of Lactic Acid Bacteria in Denaturing Gradient Gel Electrophoresis [DOI]
  12. 12 Bacteriocin production by Pediococcus pentosaceus isolated from marula (Scerocarya birrea) [DOI]
  13. 13 The spoilage characteristics of Brochothrix thermosphacta and two psychrotolerant Enterobacteriacae in vacuum packed lamb and the comparison between high and low pH cuts [DOI]
  14. 14 Isolation and characterization of Lactococcus piscium strains from vacuum-packaged refrigerated beef [DOI]
  15. 15 Antibacterial agents produced by lactic acid bacteria isolated from Threadfin Salmon and Grass Shrimp.
  16. 16 Characterization and antimicrobial activity of Pediococcus species isolated from South African farm-style cheese [DOI]
  17. 17 Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods [DOI]
  18. 18 Technological characterization of wild-typeLactococcus lactisstrains isolated from raw milk and traditional fermented milk products in Turkey [DOI]
  19. 19 Characterization of lactic acid bacteria isolated from an Italian dry fermented sausage
  20. 20 Influence of lactic cultures on the quality attributes of tsire, a West African stick meat [DOI]
  21. 21 Microbiological and physicochemical characterization of the natural fermented camel meat sausage [DOI]
  22. 22 Isolation of Bacteriocin Producing Lactococcus lactis Strain from Dairy Products* [DOI]
  23. 23 Detection and characterization of a bacteriocin produced by Lactococcus lactis subsp. cremoris R isolated from radish [DOI]
  24. 24 DNA fingerprinting of Listeria monocytogenes strains by pulsed-field gel electrophoresis [DOI]
  25. 25 Isolation of bacteriocin nisin producing Lactococcus lactis from dairy products

Cited by 5

Development of Starter Culture for the Improvement in the Quality of Ogiri, a Food Condiment

O. A. Olaoye, J. C. Ohuche, A. C. Nwachukwu · HighTech and Innovation Journal · 2022

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