The Entrapment of Paclitaxel in PLGA Nanoparticles Increases its Cytotoxicity against Multiresistant Cell Line
Vanya Bojat, Vadim Balabanyan, Renad Alyautdin
Journal of Advances in Medicine and Medical Research · pp. 306–319 · Published 3 Aug 2011
10.9734/BJMMR/2011/382Abstract
Paclitaxel (Ptx) is a taxane anticancer mitotic inhibitor, widely used in oncology for the last 20 years. Poor solubility of Ptx, as a consequence using of toxic solvents such as Cremofor EL, high affinity to P-glycoprotein are associated with serious side effects due to hypersensitivity reactions, low bioavailability and low therapeutic index. Development of new delivery solvent-free forms of Ptx is one of the key research problems in modern cancer chemotherapy. Ptx loaded into polylactic-co-glycolic acid (PLGA) nanoparticles (Ptx-PLGA-Nps) (size 200-300 nm) have been prepared using nanoprecipitation method. Impact of technological parameters on Ptx encapsulation efficacy and in vitro drug release was investigated. Drug encapsulation was determined using HPLC. Citotoxic activity and cell accumulation of nanosomal formulation of Ptx was studied on multiresistant cell line Jurkat WT (cells of human Т-limphoblastic leucosis). Obtained results suggest that formulation of PLGA Ptx nanoparticles have above 90-98% drug encapsulation efficacy, higher cell accumulation and cytotoxic activity.
References (30)
- 1 Phase III Trial of Nanoparticle Albumin-Bound Paclitaxel Compared With Polyethylated Castor Oil–Based Paclitaxel in Women With Breast Cancer [DOI]
- 2 Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay [DOI]
- 3 Protein nanoparticles as drug carriers in clinical medicine [DOI]
- 4 Paclitaxel-loaded PLGA nanoparticles: preparation, physicochemical characterization and in vitro anti-tumoral activity [DOI]
- 5 Phase I and pharmacokinetic study of ABI-007, a Cremophor-free, protein-stabilized, nanoparticle formulation of paclitaxel.
- 6 Delivery of Loperamide Across the Blood-Brain Barrier with Polysorbate 80-Coated Polybutylcyanoacrylate Nanoparticles [DOI]
- 7 Novel formulations of taxanes: a review. Old wine in a new bottle? [DOI]
- 8 Nanoparticles of Biodegradable Polymers for Clinical Administration of Paclitaxel [DOI]
- 9 Paclitaxel nanoparticles for the potential treatment of brain tumors [DOI]
- 10 Phase I and Pharmacokinetics Trial of ABI-007, a Novel Nanoparticle Formulation of Paclitaxel in Patients With Advanced Nonhematologic Malignancies [DOI]
- 11 Multicenter Phase II Trial of ABI-007, an Albumin-Bound Paclitaxel, in Women With Metastatic Breast Cancer [DOI]
- 12 Commonly used surfactant, Tween 80, improves absorption of P-glycoprotein substrate, digoxin, in rats [DOI]
- 13 Nanoparticle Albumin-Bound Paclitaxel: A Novel Cremphor-EL ® -Free Formulation of Paclitaxel [DOI]
- 14 Toxicological studies of doxorubicin bound to polysorbate 80-coated poly(butyl cyanoacrylate) nanoparticles in healthy rats and rats with intracranial glioblastoma [DOI]
- 15 Comparative scale-up of three methods for producing ibuprofen-loaded nanoparticles [DOI]
- 16 Biodistribution of polysorbate 80-coated doxorubicin-loaded [14C]-poly(butyl cyanoacrylate) nanoparticles after intravenous administration to glioblastoma-bearing rats [DOI]
- 17 Susceptibility of nanoparticle-encapsulated paclitaxel to P-glycoprotein-mediated drug efflux [DOI]
- 18 Pharmacogenetics of paclitaxel metabolism [DOI]
- 19 The effect of P-glycoprotein on paclitaxel brain and brain tumor distribution in mice.
- 20 Self-Assembled Biodegradable Nanoparticles Developed by Direct Dialysis for the Delivery of Paclitaxel [DOI]
- 21 In vivo evaluation of lipid nanocapsules as a promising colloidal carrier for paclitaxel [DOI]
- 22 In vitro synergistic, antagonistic, and duration of exposure effects of metal cations on eukaryotic cells [DOI]
- 23 Paclitaxel loaded poly(l-lactic acid) (PLLA) microspheres [DOI]
- 24 Paclitaxel-induced apoptosis in Jurkat, a leukemic T cell line, is enhanced by ceramide [DOI]
- 25 Determination of the docetaxel vehicle, polysorbate 80, in patient samples by liquid chromatography–tandem mass spectrometry [DOI]
- 26 Lyoprotected Nanosphere Formulations for Paclitaxel Controlled Delivery [DOI]
- 27 Guanine nucleosides and Jurkat cell death: roles of ATP depletion and accumulation of deoxyribonucleotides [DOI]
- 28 Application of Fluorescence Microscopy to Measure Apoptosis in Jurkat T Cells After Treatment with a New Investigational Anticancer Agent (N.C.1213) [DOI]
- 29 Update on nanoparticle albumin-bound paclitaxel.
- 30 The MRD1 (P-glycoprotein) and MRP (P-190) transporters do not play a major role in the intrinsic multiple drug resistance of Jurkat T lymphocytes [DOI]
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