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All published articles 911

Anti-bacterial Effect of Aqueous and Ethanolic Extracts of Onion, Garlic and Cinnamon on Xanthomonas Species

Amir Hussain, Tahir Naqqash, Kashif Aslam, Syed Bilal Hussain, Shahid Masood Shah & Ghulam Shabir · Biotechnology Journal International · 2021

Onion (Allium cepa), garlic (Allium sativum) and cinnamon (Cinnamomum zeylanicum) are edible herbs but they contain many chemicals which can be used as medicine. These herbs also contain many antibiotic molecules that are capable to inhibit or reduce growth of many types of micro...

Open access Research Article 10.9734/bji/2021/v25i430148

Effects of AtSPS on the Growth and Development of Arabidopsis thaliana under Abiotic Stress

Bao-zhen Zhao, Yang Yu, Zhi Yang, Qi Ding & Na Cui · Biotechnology Journal International · 2021

Aims: SPS (Sucrose phosphate synthase) participates in plant growth and yield formation, and plays an important role in plant stress resistance. This study used T-DNA insertion mutant of AtSPS in Arabidopsis as test material. The growth indexes and soluble sugar contents of Arabi...

Open access Research Article 10.9734/bji/2021/v25i430147

A Study on the Transcriptional Profile of NOS2 and IFN-γ Genes in River Buffalo with Endometritis

Othman E. Othman, Noha M. Osman, Nadia A. Abo El-Maaty & Eman R. Mahfouz · Biotechnology Journal International · 2021

Background and Aim: Uterine lumen contamination with bacteria is ubiquitous in buffalo after parturition. Nearly one-third of these infected animals develop endometritis which leads to reduced fertility. The present study aimed to evaluate the expressions of IFN-γ and NOS2 genes...

Open access Research Article 10.9734/bji/2021/v25i430146

Callus Culture as an Alternative Source of Secondary Metabolites in Curtailing Malaria Epidemic

Daniel Thakuma Tizhe, Gali Adamu Ishaku, Afiniki Yohanna, Dashe Dentsen Fortune & Aisha Salihu Jibrin · Biotechnology Journal International · 2021

Malaria remains a threat to public healthcare system. In 2018, more than 200 million people were exposed to this disease globally. There have been reports of drug-resistance in the recommended therapy in some endemic regions. This called for relentless efforts in the search for p...

Open access Research Article 10.9734/bji/2021/v25i430145

Structural Modelling and in-silico characterization of a Novel Thermophilic β-amylase from Clostridium thermosulfuregenes

Shafiqa Nayel, Mohd Shahir Shamsir & Farid Ahmad Danishfar · Biotechnology Journal International · 2021

β-amylase is a hydrolytic enzyme that is involved in breaking down starch and producing energy. Since the discovery of β-amylase, it has been applied in various applications especially in the food industry. In this study, a novel β-amylase from Clostridium thermosuluregen, a ther...

Open access Research Article 10.9734/bji/2021/v25i430144

Selection of Perspective Varieties of Cornelian Cherry in the Area of Tuzla Canton

Besim Salkić, Kemal Avdić, Ensar Salkić, Samira Huseinović, Ahmed Salkić & Sead Noćajević · Biotechnology Journal International · 2021

Organic food production contributes to the preservation of human health, preservation and protection of the environment and increase of soil fertility, reduction of all forms of pollution as a consequence of intensive agricultural production and animal husbandry. Hence by purchas...

Open access Research Article 10.9734/bji/2021/v25i330142

Effects of Groundnut Shell Incorporation Rates on the Growth and Yield of Maize (Zea mays L.) in Mubi, Adamawa State, Nigeria

I. R. Danbima, I. J. Tekwa & A. T. Gani · Biotechnology Journal International · 2021

Purpose: The aim of the study was to assess the effects of groundnut shell incorporation rates on the growth and yield of maize. Research methods: The study was carried out at the students’ demonstration farm of the Federal Polytechnic, Mubi, Adamawa State, in 2018. Seeds were so...

Open access Research Article 10.9734/bji/2021/v25i330141

Effects of Process Parameters on the Activities of Enzymes by Different Species of Aspergillus in Crude Polluted Soil Sites

J. M. Madu, A. I. Ogbonna & C. I. C. Ogbonna · Biotechnology Journal International · 2021

The effects of temperature, pH and incubation time on amylase, protease and cellulase activity by different species of Aspergillus in crude oil polluted soil sites in Nigeria were studied. Incubation period, Optimal pH values and temperatures for the enzymes produced by the diffe...

Open access Research Article 10.9734/bji/2021/v25i330140

Isolation and Identification of Resistant Bacteria from Petroleum Producing Vicinity by Gene Amplification and Sequencing

O. Aleruchi & O. Obire · Biotechnology Journal International · 2021

This investigation focuses on molecular identification of antibiotic resistant bacteria isolated from petroleum producing vicinity using 16S rRNA sequencing based technique. The bacterial 16s rRNA gene sequences were amplified using polymerase chain reaction, sequenced,  characte...

Open access Research Article 10.9734/bji/2021/v25i330139

Cellulase: Distribution, Production, Characterization and Industrial Applications

Pratibha Maravi & Anil Kumar · Biotechnology Journal International · 2021

Cellulase enzyme complex is comprised of three enzymes namely exo-glucanase, endo-glucanase and β-glucosidase which act synergistically to deconstruct cellulosic biomass in order to produce fermentable sugars. The enzymes are produced naturally by the living organisms such as bac...

Open access Research Article 10.9734/bji/2021/v25i330143

Effect of Pre and Post Infection Administration of Zinc and Selenium on some Biochemical indices in Wistar Rats infected with Trypanosoma brucei

Timothy Tese, Kola Matthew Anigo, Alice Onyeche Jonah, Meshack Anthony Kugama, Tinok Andrew, Janet Larai Ibrahim, Raymond Arumona & William Denbon Genesis Chinten · Biotechnology Journal International · 2021

Aims: The effect of pre and post-infection administration of zinc and selenium on Trypanosoma brucei brucei infection in wistar rats on some biochemical parameters were investigated. Study Design:  The study was designed to evaluate the effect of pre and post infection administra...

Open access Research Article 10.9734/bji/2021/v25i230138

Genetic Characterization of Domestic Apple Varieties from Tuzla Canton, BiH

Ensar Salkić, Besim Salkić, Amela Hercegovac, Aldijana Avdić, Azra Dorić & Emir Imširović · Biotechnology Journal International · 2021

Aims: The basic precondition for apple breeding is the genetic diversity of varieties, which implies a large number of different, positive genes that enable adaptation to different weather conditions, resistance to new diseases and pests. One of the reliable sources of genetic di...

Open access Research Article 10.9734/bji/2021/v25i230137

Optimization of Selected Process Parameters Affecting Yield of Green Synthesized Silver Nanoparticles and Their Antibacterial Activity

F. A. Ekaji, C. O. Akujobi & S. I. Umeh · Biotechnology Journal International · 2021

Aims: To optimize effects of selected process parameters affecting yield of green synthesized silver nanoparticles and their antibacterial activity. Study Design: Study was designed with 3 factors Box Behnken Design (Minitab 17) and Response optimizer (Minitab 17) was used to det...

Open access Research Article 10.9734/bji/2021/v25i230136

Construction of an Agrobacterium Mediated RNAI Genetic Transformation Vector Targeting the Replicase Gene of Indian cassava mosaic virus and Evaluation of Their Transformation Ability in Cassava Immature Leaf Lobes

T. Anuradha & P. Prabha · Biotechnology Journal International · 2021

Cassava mosaic virus is one of the major problems affecting cassava industry in India. Currently there are no effective strategy to completely protect cassava from cassava mosaic viruses. In order to attain cassava mosaic virus resistance RNAi vectors targeting the replicase gene...

Open access Research Article 10.9734/bji/2021/v25i230135

Molecular Detection and Typing of Cervical Human Papillomavirus in a Selected Population in Bayelsa State: Comparison with the National and International Trend and Clinical Significance

Y. I. Oboma, A. A. Ngokere, Y. M. Tatfeng, S. I. Musa, I. A. Ibrahim, S. E. Itodo & A. B. Abdu · Biotechnology Journal International · 2021

Background: The link between cervical lesions and human Papillomavirus (HPV) 16 and 18 is well established, but the magnitude of the risk of association and the importance of other high-risk hpv types is uncertain in Bayelsa state. Aims: The study was aimed at detecting and typin...

Open access Research Article 10.9734/bji/2021/v25i230134