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All published articles 911

In Vitro Regeneration of Banana Cv. Nanjanagud Rasabale (AAB) by Shoot Tip Culture

Prabhuling Guranna, Rashmi Hegde & Babu Ammanaghatta Govindaswamy · Biotechnology Journal International · 2018

The cultivar Nanjanagud Rasabale (Musa spp. AAB, Silk subgroup) geographical indication tagged and leading cultivar of Mysore district. It is known for unique taste, has a huge demand across the country. In vitro regeneration in banana is preferred for faster multiplication rate...

Open access Research Article 10.9734/BJI/2017/39892

Non-target Host Immune Gene Modulation in Transgenic Silkworm Bombyx mori Endowed with RNAi Silence BmNPV Genes

Burdekar Varada, Appukuttan Nair R. Pradeep, Arvind K. Awasthi, Vankadara Sivaprasad, Kankayam M. Ponnuvel & Rakesh K. Mishra · Biotechnology Journal International · 2018

Aim: To reveal differential expression of host- response genes activated after nuclear polyhedro virus infection in transgenic silkworm Bombyx mori larva and to show an influence of ‘BmNPV transgenes’ on expression pattern of host- response genes. Study Design: Relative expressio...

Open access Research Article 10.9734/BJI/2017/39442

Biodegradation of Indeno (1, 2, 3 - c, d) Pyrene and Dibenzo (A, H) Perylene by Aerobic Heterotrophic Bacteria and Cyanobacteria in Brackish Water

Ichor, Tersagh, E. M. Aondoakaa & Ande, Sesugh · Biotechnology Journal International · 2018

Biodegradation of indeno (1, 2, 3- c, d) pyrene and dibenzo (a, h) perylene; high molecular weight PAHs  by isolates of aerobic heterotrophic bacteria, cyanobacteria and its consortium from Boodo Creek characterized with brackish water was monitored for 56 days using GC- MS. The...

Open access Research Article 10.9734/BJI/2017/33950

Evaluation of Reproductive Characteristics and Feed Utilization of Two Strains of Heterobranchus bidorsalis (Geoffrey, 1809)

L. U. Onyia, C. Duru, K. G. Michael & S. D. Duwal · Biotechnology Journal International · 2018

Intraspecific hybridization studies were carried out in two strains of Heterobranchus bidorsalis obtained from two ecological zones in Nigeria – Yola (Sudan savannah) and New Bussa (Guinea savanna) with the aim of evaluating the fecundity, percentage fertilization, hatchability,...

Open access Research Article 10.9734/BJI/2017/38472

Micropropagation in Pomegranate (Punica granatum L.) cv. ‘Bhagwa’ through Indirect Organogenesis and Assessment of Genetic Fidelity by RAPD Marker

Prabhuling Guranna, Iranna Hosamani, Raghavendra Sathyanarayana, Rashmi Hegde & Kulapati Hipparagi · Biotechnology Journal International · 2018

Pomegranate (Punica granatum L.) is an ancient fruit crop belonging to the family Punicaceae. Conventionally, pomegranate is propagated through air layering, hard wood and semihard wood cuttings. Recently micropropagation is gaining popularity due to rapid mass propagation of dis...

Open access Research Article 10.9734/BJI/2017/38806

Application of Metagenomic Study for the Detection of Diphtheria Toxin Gene of Corynebacterium diphtheriae in Patients Displaying Symptoms of Pharyngitis in Kaduna State

M. I. Bindawa, M. S. Abdulsalami, Y. O. Abdul Akeem, A. A. Jabbo, M. Bello & A. B. Suleiman · Biotechnology Journal International · 2018

This study was set to detect the A and B subunit of Diphtheria toxin gene among patients with pharyngitis and to identify which of the possible biovars is endemic in Kaduna State. The result of standard collection of twenty-two (22) throat swab samples of patients using standard...

Open access Research Article 10.9734/BJI/2017/37342

Effects of Length of Fermentation and Storage Media on Chemical Properties of Palm Oil

T. A. Ayelegun & P. O. Ajewole · Biotechnology Journal International · 2018

This study examined the effects of different length of fermentation and storage media on quality characteristics of palm oil produced at the processing centre of Department of Agricultural and Bio-Environmental Engineering, The Federal Polytechnic, Ado-Ekiti, Nigeria. Fresh palm...

Open access Research Article 10.9734/BJI/2017/37393

Use of Gene Specific Universal Primers for Isolation of DNA Sequences Encoding Laccase Enzyme from a Wild Isolate of Schizophyllum commune

Vidya Pradeep Kumar, Atul P. Kolte, Arindam Dhali, Chandrashekar Naik & Manpal Sridhar · Biotechnology Journal International · 2017

Laccase enzymes plays a vital role in innumerable biotechnological applications and hence their large scale production has stimulated considerable research. In the present study, degenerate universal primer pairs were employed to isolate laccase gene from a wild isolate of laccas...

Open access Research Article 10.9734/BJI/2017/37022

Comparative Analysis of DNA Extraction Methods in Two Popular Varieties of Finger Millet (Eleusine coracana) from Ethiopia

Aklilu Banjaw Leza, Sunil Tulshiram Hajare & Nitin M. Chauhan · Biotechnology Journal International · 2017

Aims: The aim of this investigation was to standardize the genomic DNA isolation protocol to obtain high quality and quantity DNA for genomic analysis. To accomplish this task, dry leaves of finger millet which are rich in polysaccharides and polyphenols and seeds were utilized a...

Open access Research Article 10.9734/BJI/2017/33145

Prokaryotic Diversity of the Alkaline Lake Acıgöl, Turkey by Using Culture-dependent and Culture-Independent Methods

Gamze Başbülbül, Haci Halil Biyik, Erman Oryaşın & Bülent Bozdoğan · Biotechnology Journal International · 2017

Aims: The prokaryotic diversity of Acıgöl lake, Turkey, was studied in samples taken from soil, water and mud. Culture -dependent and -independent methods were used to analyse the results. Methodology: Bacterial cultures were isolated by using six different growth media. Isolates...

Open access Research Article 10.9734/BJI/2017/36909

Potentialities of Yeast Strains to be Used as Freeze-Drying Starters for the Production of Traditional Sorghum Beer in Côte d’Ivoire

Wahauwouélé Hermann Coulibaly, Fatoumata Camara, Djegba Marie Toka, Koffi Maïzan Jean-Paul Bouatenin, Alfred K. Kouamé, Youan Charles Tra Bi, Anne Abot & Koffi Marcellin Djè · Biotechnology Journal International · 2017

Freeze-drying is a well-known dehydration method widely used to preserve microorganisms. In order to produce freeze-dried yeast starter culture for the brewing purpose of African traditional sorghum beer, we tested tolerance to ethanol stress and evaluated the relative expression...

Open access Research Article 10.9734/BJI/2017/36851

Molecular Markers and Postulation Study of Leaf Rust Resistance Genes in Various Egyptian Wheat Cultivars

Hanaa Abouzied, Eman El Argawy & Walid El-Orabey · Biotechnology Journal International · 2017

The current investigation was carried out at seedling stage under greenhouse condition to: (1) postulate leaf rust resistance genes in ten Egyptian spring wheat cultivars at seedling stage and (2) Verify the presence of resistance genes in the selected cultivars using specific mo...

Open access Research Article 10.9734/BJI/2017/36175

Production and Characterization of Halo Tolerant Alkaline Protease of Enterobacter aerogenes Strain ZB and Its Application

Mohammed A. Almalki, D. S. Deepa Dhas, Rakesh Varghese & Ponnuswamy Vijayaraghavan · Biotechnology Journal International · 2017

Aims: The present investigation was carried out to isolate a halo tolerant Enterobacter aerogenes strain ZB for the production of alkaline protease to degrade protein wastes from the tannery wastewater. Study Design: Halo tolerant protease producing bacteria was isolated from the...

Open access Research Article 10.9734/BJI/2017/37466

In vitro Salinity Evaluation Studies in Golden Berry (Physalis peruviana L.)

Fatma Burcu Celikli, Pınar Akkelle & Ahmet Naci Onus · Biotechnology Journal International · 2017

Aim: Present study, therefore, was conducted in in vitro conditions to study effect of NaCl at varied levels on growth and chlorophyll content of golden berry shoot apices grown in in vitro conditions. Place and Duration: The study was carried out in the Department of Horticultur...

Open access Research Article 10.9734/BJI/2017/37097