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All published articles 911

Molecular Differentiation of Five Quinoa (Chenopodium quinoa Willd.) Genotypes Using Inter-simple Sequence Repeat (ISSR) Markers

A. M. M. Al-Naggar, R. M. Abd El-Salam, A. E. E. Badran & Mai M. A. El-Moghazi · Biotechnology Journal International · 2017

Knowledge of genetic diversity is one of the important tools used for genetic management of quinoa accessions for plant breeding. This research aimed to molecularly characterize five quinoa genotypes using ISSR markers to reveal genetic polymorphism and identify unique markers fo...

Open access Research Article 10.9734/BJI/2017/37053

Relationship between Neutral Invertase Activity and Sugar Contents in Tomato Fruit and Its Functional Prediction Analysis

Wang Hao-Ran, Yao Jia-Yu, Zhang Tong-Tong, Cui Na, He Sheng, Zhang Rui & Xu Si-Qiong · Biotechnology Journal International · 2017

Aims: Neutral invertase (NI) probably plays an important role in sucrose metabolism of tomato, but main function and mechanism are unclear. In this study, contents of soluble sugar and NI activity were measured at different stages in tomatoes of Solanum chmielewskii and Solanum l...

Open access Research Article 10.9734/BJI/2017/37195

Isolation, Identification and Characterization of Cellulase from a Bacterium Obtained at a Saw-Mill Site in Ile-Ife, Nigeria

A. E. Agbolade, E. F. Aransiola, K. O. Awojobi & M. K. Bakare · Biotechnology Journal International · 2017

Cellulose continues to account for one of earth’s most abundant biomass. Cellulase degrades cellulose, thereby making it one of the most sought after enzyme in the commercial market. This research aimed to characterize cellulase with enviable physicochemical parameters from a bac...

Open access Research Article 10.9734/BJI/2017/37221

Optimization and Lipase Production of Lysinibacillus sphaericus in Domestic Oil Rich Waste Water

Babatunde Aderiye & Adebisi Sulaimon · Biotechnology Journal International · 2017

The present study investigated the growth and lipase production by Lysinibacillus sphaericus in a minimal medium. The growth conditions (i.e oil concentration, nitrogen source, carbon source, metals and non metals) were optimized for maximum production of enzyme. Influence of dif...

Open access Research Article 10.9734/BJI/2017/34290

Inhibitory Effects of 4T1 Breast Tumor Transplantation on Mouse Peripheral Blood Immune Cell Populations

Kexin Zheng, Qilong Li, Chenghao Fu & Shiliang Ma · Biotechnology Journal International · 2017

Aims: One of serious threats to women's health is mammary cancer whose occurrence, development, and treatment are related to the body’s immunological circumstances. In addition, the cancer also imposes the some effects on the body’s immune system. However, the body’s response is...

Open access Research Article 10.9734/BJI/2017/36382

Optimisation of Growth Factors for Effective Use of Phosphate Solubilizing Bacterial Strains and Its Use as Bioinoculants for the Growth of Groundnut (Arachis hypogaea) Plant

Priyanka Anbalagan & Veena Gayathri Krishnaswamy · Biotechnology Journal International · 2017

Phosphorus (P) is one of the essential macronutrients for plant growth. Phosphate solubilizing bacteria (PSB) are organisms which are used as bioinoculants to enhance the plant growth. Plants take phosphate in the form of soluble orthophosphate ions but due to the presence of cal...

Open access Research Article 10.9734/BJI/2017/36243

RNA-Seq Evaluating Several Custom Microarrays Background Correction and Gene Expression Data Normalization Systems

Noel Dougba Dago, Martial Didier Yao Saraka, Nafan Diarrassouba, Antonio Mori, Hermann-Désiré Lallié, Edouard Kouamé N’Goran, Lamine Baba-Moussa, Massimo Delledonne & Giovanni Malerba · Biotechnology Journal International · 2017

Microarray gene expression technologies represents a widely used tool in transcriptomics and genomics studies worldwide. Even if this technology exhibits a low dynamic range as well as a feeble sensitivity and specificity (limited performances) with respect to RNA sequencing (RNA...

Open access Research Article 10.9734/BJI/2017/36345

Investigation on the Effects of UV Radiation on Physiological Characteristics of Moringa oleifera Lam. in vitro and in situ

Asma G. Oraibi · Biotechnology Journal International · 2017

Moringa oliefera seeds were treated with UV light type A, B and C for 30 minutes. Seedlings length, number of plants, number of axillary buds, number of adventitious buds and number of apical buds were recorded 34 days after sowing the treated seeds in addition to the control in...

Open access Research Article 10.9734/BJI/2017/35996

Impact of High Glycine Tyrosine KAP Genes on Cashmere Fibre Trait Characteristics

Aadil Ayaz, Zaffar Iqbal, Basharat Ahmad, Nazir Ahmad Ganai, N. Singh, Aarif Ali, Tajamul Mumtaz, Mashooq Ahmad Dar, Mir Shabir, Syed Shanaz & Suheel Yousuf Wani · Biotechnology Journal International · 2017

Cashmere goat (Capra hircus) represents a world renowned goat famous for production of superfine downfibre “Cashmere”. The worldwide total production of cashmere fibre is about 10000-15000 tonnes per annum. Among all the fibres cashmere is the finest fibre of animal origin, produ...

Open access Research Article 10.9734/BJI/2017/35210

Assessment of the Influence of Transgenic Cotton on Beneficial Soil Rhizosphere Microbes

Sivaji Mathivanan & Sridar Raghupathi · Biotechnology Journal International · 2017

In this study the impact of Bt cotton has been accessed on the population of beneficial microbes in the rhizosphere of transgenic Bt cotton line (RCH-2 BG II) and its counterpart (a non-Bt cotton line). The Bacillus thuringiensis (Bt) proteins i.e., Cry1Ac and Cry2Ab were release...

Open access Research Article 10.9734/BJI/2017/32592

Effect of Bacillus subtilis TLO3 Amylase Pre-treatment on Ethanol Production from Raw Starches

Slimane Choubane & Ben Amar Cheba · Biotechnology Journal International · 2017

Bioethanol is currently the most widely used liquid biofuel in the world. Starch rich crops occupy the first place as biomass for bioethanol production. Amylases (EC 3.2.1.1) are enzymes that hydrolyses starch into sugar units, and pre-treating starch with amylolytic bacteria or...

Open access Research Article 10.9734/BJI/2017/35030

Performance of Broad Bean (Vicia faba L.) Genotypes for Pod Yield and Yield Contributing Morpho-physiological Components under Mid Hill Conditions of North Western Himalayas

Yudhvir Singh, Simran Sharma, Bhallan Singh Sekhon, Arti Verma & Surbhi Sharma · Biotechnology Journal International · 2017

Aims: The present investigation was planned to evaluate thirty five broad bean genotypes along with one check Vikrant-B for yield and some yield contributing morpho-physiological components. Study Design: Randomized Block Design. Place and Duration of Study: The experiment was un...

Open access Research Article 10.9734/BJI/2017/35354

Phytochemical and Antimicrobial Screening of Root Extracts of Dacryodes edulis

P. J. Nna, T. A. Tor-Anyiin, J. O. Igoli, M. E. Khan & J. V. Anyam · Biotechnology Journal International · 2017

Aims: To screen extracts of Dacryodes edulis phytochemically. To assay the said extracts for antimicrobial activity. To validate or otherwise claims by traditional medicine practitioners on Dacryodes edulis. Place and Duration of Study: Department of chemistry, University of Agri...

Open access Research Article 10.9734/BJI/2017/35311

Novel Method for Detection, Quantification and Cytopathic Changes in Developed Epithelial Cell Line with Human Papillomaviruses-16 Infection

Rahela Ibrahim, Kavindra Pratap Singh, P. W. Ramteke, Mamta Singh, Laxmi Shankar Singh & Dharmendra Kumar Chaudhary · Biotechnology Journal International · 2017

Human papillomaviruses (HPVs) belong to the class of DNA viruses which are known to cause different human cancers. Development of new diagnostic methods and tools to study virus biology and mechanism of infection had been strongly felt in order to prevent HPV infections. In the p...

Open access Research Article 10.9734/BJI/2017/34968