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Joint Effects of Water-Miscible Solvents on Kluyveromyces marxianus Cell-Free β-Galactosidase Activity

Oluchukwu Roseline Nweke, Christian Okechukwu Nweke, Christian Chibuzor Opurum, Obioma Kelechi Mejeha, Chinonso Judith Pius & Chidimma Precious Onyeka · Biotechnology Journal International · 2026

Aim: The study aims to assess the inhibitory effects of ethanol-N,N-dimethylformamide (DMF) and ethanol-dimethyl sulfoxide (DMSO) binary mixtures on Kluyveromyces marxianus cell-free β-galactosidase activity. Study design: A cell-free enzyme assay integrating concentration-respon...

Open access Research Article 10.9734/bji/2026/v30i4888

Precision Genetic Engineering for Crop Improvement in Modern Agriculture: A Critical Review

D. Mohan Krishna, M. Vamshi & Roshan Mohiddin · Biotechnology Journal International · 2026

Global agriculture is being squeezed from several directions at once: population growth continues, climate variability is accelerating, arable land per capita is shrinking, and micronutrient malnutrition persists alongside outright hunger in many regions. Conventional breeding an...

Open access Research Article 10.9734/bji/2026/v30i3885

Optimization of Artemisinin Accumulation in Artemisia annua L. Green Callus Cultures: Impact of Media, Elicitors and Environmental Factors

Mai Thi Phuong Hoa, Đo Tien Vinh, Pham Hong Diep & Tran Van Minh · Biotechnology Journal International · 2026

Introduction: Artemisinin is a sesquiterpene lactone with an endoperoxide bridge isolated from Artemisia annua L. It is effective against chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum malaria parasites and is used in the treatment of drug-resistant malaria...

Open access Research Article 10.9734/bji/2026/v30i3886

Multi-target Computational Profiling of Caffeine and Vernonia amygdalina Phytoconstituents Reveals Potential Neuroprotective Modulators of Diabetes-Associated Neurodegeneration

B. C. Robinson, B. C. Chinko, V. O. Hart & S. O. Ojeka · Biotechnology Journal International · 2026

Diabetes-associated neurodegeneration involves multiple pathological processes, including oxidative stress, impaired neurotrophic signalling, neurotransmitter dysregulation, altered metabolic signalling and progressive neuronal dysfunction. This study evaluated the in silico inte...

Open access Research Article 10.9734/bji/2026/v30i3884

The Effect of the Ethanolic Leaf Extract of Senna alata on Biochemical and Haematological Parameters in Albino Rats

S. M. Midawa, T. D. Tizhe, S. Y. Comfort, B. M. Zainab, S. Israel & I. S. Albert · Biotechnology Journal International · 2026

Senna alata is a medicinal plant in the family Fabaceae and is widely used in traditional medicine for the management of several conditions. Although the plant is commonly used, information on its haematological and biochemical effects remains limited. This study assessed the eff...

Open access Research Article 10.9734/bji/2026/v30i3883

Production of Copper Nanoparticle-Embedded Chitosan Hydrogel for Potential Application in Wound Management

Aminu Argungu Umar, Ibrahim Sani, Ahmad Ibrahim Bagudo & Shamsudeen Aliyu · Biotechnology Journal International · 2026

Nanoparticle-embedded hydrogels present a novel therapeutic approach in wound management. These hydrogels are multifunctional, have efficacy and their production is practicable. Hence, this work focused on the formulation and characterization of copper nanoparticles-embedded chit...

Open access Research Article 10.9734/bji/2026/v30i3882

Optimization of Culture Conditions for Arthrospira platensis Cultivation in Closed Tubular Photobioreactors

Phan Van Dan, Nguyen Hoang Dung, Do Dang Giap, Le Quynh Loan, Tran Thi My Ngoc, Vu Thi Tuyet Nhung, Tran Quang Vinh, Nguyen Thi Thuy & Tran Trung Kien · Biotechnology Journal International · 2026

Background: Tubular photobioreactors (PBRs) enhance high-quality Arthrospira platensis biomass production. However, microalgae are highly susceptible to micro-environmental fluctuations, specifically the combined influence of photo-thermal stress, dissolved oxygen (DO) accumulati...

Open access Research Article 10.9734/bji/2026/v30i3881

Identification and Characterisation of Shiga Toxin-producing and Enteropathogenic Escherichia coli in Cattle Hides Sold in Markets in Bobo-dioulasso, Burkina Faso

Sonagnon H. S. Kouhounde, Juste Sanou, Joseph Makaya, Lamine Baba-Moussa, Mamoudou H. Dicko & Michel K. Gomgnimbou · Biotechnology Journal International · 2026

Background: In Burkina Faso, bovine hide, constitutes an important component of the diet and represents a valued source of animal protein. However, the slaughtering, processing, and marketing practices associated with this product often occur under inadequate hygienic conditions,...

Open access Research Article 10.9734/bji/2026/v30i3880

In vitro Antimicrobial Activity of Ethanolic and Methanolic Leaf Extracts of Ocimum gratissimum against Multidrug-Resistant Escherichia coli Clinical Isolates

F. A. Adewumi, J. O. Ipinlaye, A. I. Airaodion & A. O. Oluyege · Biotechnology Journal International · 2026

Background: The rapid emergence and spread of multidrug-resistant (MDR) Escherichia coli isolates have become a major global public health concern, limiting the effectiveness of conventional antibiotics and increasing treatment failure rates. Consequently, medicinal plants such a...

Open access Research Article 10.9734/bji/2026/v30i3879

Harnessing CRISPR/Cas9 for Rapid Genetic Improvement of Tomato: From Trait Enhancement to Stress Resilience

K. Lakshmi Jayaraj & Smita Nair · Biotechnology Journal International · 2026

Tomato (Solanum lycopersicum L.) is a globally important vegetable crop, widely cultivated and consumed due to its rich nutritional profile, including vitamins, carotenoids, and phenolic compounds that contribute to human health. However, its productivity is significantly constra...

Open access Research Article 10.9734/bji/2026/v30i3878

Immunomodulatory Effects of Quercetin on Crab Allergen-Induced Responses in THP-1 Derived Macrophages

Renu Indhikkattu Chittoor & Harikumaran Thampi Balakrishnan Saraswathi · Biotechnology Journal International · 2026

A serious and growing food hypersensitivity, shellfish allergy is mainly brought on by allergenic proteins like arginine kinase and tropomyosin.  As of right now, allergy avoidance and symptomatic therapy are the main therapeutic approaches because there is no proven cure. Althou...

Open access Research Article 10.9734/bji/2026/v30i3877

From Contamination to Functional Shift: Agrochemical Effects on Soil Microbial C:N:P Balance and Enzymatic Activity

Tega Lee-Ann ATAIKIRU & Emmanuella Oghenevavwarero Onosemuode · Biotechnology Journal International · 2026

Background: Agrochemicals, while vital for enhancing agricultural productivity, are increasingly recognized as environmental pollutants, impacting soil health and microbial ecosystems. Aim: This study examined the microbial biomass carbon (MB-C), nitrogen (MB-N), phosphorus (MB-P...

Open access Research Article 10.9734/bji/2026/v30i3876

Effect of Natural Plant-based Bio stimulants on the Growth and Protection of Cocoyam (Xanthosoma sagittifolium L. Schott)

Damien Fabrice Wassom, Mouhamed Noudi Lijouom, Gaston Mbang Elock, Rosine Barakielle Arckange Abah Bindzi, Arouna Meshuneke, Nicolas Niemenak & Cécile Annie Ewane · Biotechnology Journal International · 2026

Background: Cocoyam (Xanthosoma sagittifolium L. Schott) is an important staple crop for food security worldwide. However, its cultivation faces several constraints, including the limited availability of planting material in both quantity and quality, as well as pests, diseases (...

Open access Research Article 10.9734/bji/2026/v30i3875

Bioinformatics Analysis and Expression Profiling of the Jasmonic Acid-responsive Transcription Factor SlMYB83

Zijuan Huang, Yuanyuan Peng, Binbin Yin, Na Cui, Yang Yu & Juyong Zhao · Biotechnology Journal International · 2026

Background: Tomato (Solanum lycopersicum) stress responses are strongly regulated by jasmonic acid signaling—especially via MYB transcription factors—yet key regulators like SlMYB83 in JA-deficient spr2 mutants remain largely uncharacterized. Aims: Based on the tomato SlMYB83 gen...

Open access Research Article 10.9734/bji/2026/v30i3874

Bio-Fabrication of a Biodegradable Flower Vase from Sawdust Using Fungal Mycelium: Dual Function for Spent Mushroom Substrate

C.O. Bamigboye, A.G. Adewoyin, E.O. Olokun, M.J. Falade, M.O. Oladimeji, B. Olatunde & G.O. Bamigboye · Biotechnology Journal International · 2026

The growing environmental burden of plastic waste necessitates sustainable alternatives derived from renewable resources. Therefore, mycelium-based composites have emerged as promising biomaterials. The main objectives of the study is to develop a biodegradable flower vase. A myc...

Open access Research Article 10.9734/bji/2026/v30i2872