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All published articles 911

Computational Insight into PCR Amplified Pectate Lyase Genes from Different Species of Aspergillus

Manish Kumar, Jeya Nasim, Sangeeta Yadav, Aiman Tanveer & Dinesh Yadav · Biotechnology Journal International · 2016

Pectate lyase (PL) is an important member of pectinase group of enzyme associated with cleaving α -1, 4 linkages in pectate polymers by a β-elimination mechanism, producing 4, 5–unsaturated oligogalacturonates. In the present study, PCR amplification of pectate lyase genes from d...

Open access Research Article 10.9734/BJI/2017/29140

Transferability of Expressed Sequence Tag of Single Sequence Repeats Markers of Tomato Fruit in Different Vegetable Varieties

Na Cui, Yicong Shang, Jiayu Yao, Tongtong Zhang, Shuyu Pang, Yang Yu & Haiyan Fan · Biotechnology Journal International · 2016

Aims: The expressed sequence tag of single sequence repeats (EST-SSR) primers designed from tomato fruit were studied for their polymorphism and transferability to other vegetable varieties. Study Design: Primers were designed from ESTs in which the repeat bases of SSR were more...

Open access Research Article 10.9734/BJI/2017/30341

Mutagenicity of Oil Drilling Fluid (Potassium Chromate) on the Seedlings of Vigna unguiculata L. (Walp). in the Niger Delta, Nigeria

Ajah Obiageri Florence & Obute Gordian Chibuzo · Biotechnology Journal International · 2016

The morphological, leaf epidermal and anatomical distortions caused by the exposure of Vigna unguiculata L. to potassium chromate was investigated using 1-4%w/w of an oil field chemical Potassium chromate and Control (0). Results showed that 2-4%w/w concentrations elicited notice...

Open access Research Article 10.9734/BJI/2017/28755

Identification and Enzymatic Potential of Bacillus Species Isolated from Traditional Cassava Starters: Potential for Attiéké Production

Charlotte Ehon, Micael Bedikou, Souleymane Soumahoro & Sébastien Niamké · Biotechnology Journal International · 2016

Aims: Microbial enzymatic activities are important in cassava dough fermentation for attiéké production. The objective of this study was to describe the molecular identification and the amylolytic, pectinolytic and cellulolytic enzymes potential of four (4) Bacillus species invol...

Open access Research Article 10.9734/BJI/2017/29688

The Screening of EST-SSR Markers Associated with Peel Color in Tomato Fruit

Na Cui, Jiayu Yao, Yicong Shang, Tongtong Zhang, Shuyu Pang, Yang Yu & Haiyan Fan · Biotechnology Journal International · 2016

Aims: The color of tomato fruit is an important property in improving of tomato breed. So we screened the differences of yellow tomato peel and transparent tomato peel using EST-SSR markers, and it is in favour of the research of the tomato fruit color and tomato breeding. Study...

Open access Research Article 10.9734/BJI/2016/29881

Isolation and Characterization of α –Amyrin from Stem Bark of Ficus exasperata (Vahl)

L. A. Nnamonu, T. A. Tor-Anyiin, N. O. Ugbenyo & J. V. Anyam · Biotechnology Journal International · 2016

Aims: Isolate and characterize compounds from the ethyl acetate stem bark extract of Ficus exasperata. Place and Duration of Study: Department of Chemistry and Centre for Agrochemical Technology, University of Agriculture Makurdi, Nigeria; Herbarium of the Department of Biologica...

Open access Research Article 10.9734/BJI/2016/29737

In vitro Antibacterial Activity of Organic Extracts from North-West Moroccan Medicinal Plant Myrtus communis (L.)

Abdelhakim Bouyahya, Jamal Abrini, El Ouardy Khay, Saoulajan Charfi, Nadia Boujida, Abdeltif EL Harsal, Ahmed Talbaoui, Abdeslam Et-Touys, Youssef Bakri & Nadia Dakka · Biotechnology Journal International · 2016

Aims: This study was designed to evaluate the antibacterial activity of methanol, ethanol, ethyl acetate and n-hexane extract from the leaves of Myrtus communis (L.) growing in North-West of Morocco. Study Design: In vitro evaluating of antibacterial activity and determination of...

Open access Research Article 10.9734/BJI/2016/28500

Effects of Input Variables on the Conversion of 5 Ton/h Processed Tomato Juice in a Triple-Effect Evaporator

Mojirade M. Oloruntoba, B. A. Olunlade & F. P. Ibitoye · Biotechnology Journal International · 2016

Tomato is grown in many regions of the world; and processed tomato products account for 80% of total tomato consumption. A worldwide tomato processing industry has since evolved with a range of technologies which are able to convert tomatoes into products such as paste, sauces an...

Open access Research Article 10.9734/BJI/2016/29320

Amylase Producing Bacteria from Ensete ventricosum (Welw) Cheesman (Ensete) and Their Abilities to Hydrolyse Starch in a Locally Designed Bioreactor

Asefa Niguse, Yohannes Kassaye, Fertuna Shemsedin, Umer Ahmed, Abreham Bekele & Musin Kelel · Biotechnology Journal International · 2016

Enset is a herbaceous perennial crop used as a basic diet for millions of people at various agro ecological settings of Ethiopia. As it takes up the nutrients from the soil environment, it makes mutual relation with rhizospheric microorganism which can contribute to the discovery...

Open access Research Article 10.9734/BJI/2016/29388

Molecular Characterization of Orobanche crenata in Egypt Using ISSR Markers and Its Relation to Faba Bean Breeding

M. M. F. Abdalla, M. M. Shafik, Sabah M. Attia & Hend A. Ghannam · Biotechnology Journal International · 2016

Orobanche crenata is an annual parasitic weed that causes heavy losses to its host crop faba bean (Vicia faba). Determining the genetic diversity in Orobanche germplasm is a preliminary crucial step in faba bean breeding.It helps in identifying liable criteria of host tolerance....

Open access Research Article 10.9734/BJI/2016/27216

Assessing the Genotoxic Effects of Aqueous Leaf Extract of Neem (Azadirachta indica A. Juss), Cosmetics and Alomo Bitters (Alcoholic Drink) Using Allium Test

G. C. Obute, C. Ekeke & U. A. Nwagwu · Biotechnology Journal International · 2016

Genotoxic effect of aqueous leaf extract of Neem (Azadirachta indica) NZI, Alomo bitters alcoholic drink (ABAD) and cosmetics (Baby-face facial cleanser, BFFC; Peau clair body lotion, PCBL and Vanilla essence roll-on, VERO) were assessed using Allium test. The root tips were trea...

Open access Research Article 10.9734/BJI/2016/29042

Plant Regeneration and Its Functional Analysis within Transgenic Rice of ASG-1, an Apomixis-specific Gene Isolated from Apomictic Guinea Grass

Lanzhuang Chen, Liming Guan, Daiki Toyomoto, Toru Sugita, Takuro Hamaguchi & Reiji Okabe · Biotechnology Journal International · 2016

Aims: To analyze the function of the ASG-1, an apomixis-specific gene has been isolated from apomictic guinea grass, the combination of Agrobacterium-mediated transformation system and pSMA35H2-NG binary vector were used for transformation of ASG-1 to ‘Nipponbare’ of rice, and it...

Open access Research Article 10.9734/BJI/2016/29146

Insilico Prediction of T-cell Epitopes to Therapeutic Interferon -Beta (IFN-β) Protein

Swathi Krishna Reddy & Venkata Bharat Kumar Pinnelli · Biotechnology Journal International · 2016

Aims: Several studies have reported the existence for T helper cell epitopes with the persistence of unwanted immune reactions for several protein drugs. T-cell epitope is an amino acid or set of amino acids that are capable of being recognized form one or more T-cell receptors....

Open access Research Article 10.9734/BJI/2016/29184

Biddulphia (Odontella) sp. Isolated from the Seawater of Kayalpattinam Coastal Area, Gulf of Mannar and Maintained in Tuticorin Research Centre of ICAR-CMFRI

S. Lakshmana Senthil, C. P. Suja, B. Koncies Mary & P. Vijayagopal · Biotechnology Journal International · 2016

Aim: Present study was aimed to isolate marine algae from the sea water samples of Kayalpattinam Coastal area, Gulf of Mannar. Methodology: Seawater samples were collected for the isolation of phytoplankton from the Kayalpattinam Coastal area, Tuticorin on May 2016. The collected...

Open access Research Article 10.9734/BBJ/2016/29300