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Starch Phosphorylase: An Overview of Biochemical Characterization, Immobilization and Peptide Mapping

Ritu Jain, Sarika Garg & Anil Kumar · Biotechnology Journal International · 2014

Starch Phosphorylase: An Overview of Biochemical Characterization, Immobilization and Peptide Mapping Starch phosphorylase (EC 2.4.1.1) is the key enzyme which catalyzes the reversible conversion of starch and inorganic phosphate into glucose-1-phosphate (G-1-P) and has been repo...

Open access Research Article 10.9734/BBJ/2015/14522

Production of Bioethanol from Wild Cassava Manihot glaziovii through Various Combinations of Hydrolysis and Fermentation in Stirred Tank Bioreactors

Anselm P. Moshi, Ken. M. M. Hosea, Emrode Elisante, Anthony Manoni Mshandete & Ivo Achu Nges · Biotechnology Journal International · 2014

Production of Bioethanol from Wild Cassava Manihot glaziovii through Various Combinations of Hydrolysis and Fermentation in Stirred Tank Bioreactors Aim of the Study: The aim of this study was to evaluate three ethanol fermentation approaches namely (I) separate hydrolysis and co...

Open access Research Article 10.9734/BBJ/2015/13981

Fatty Acid Composition and Physicochemical Properties of Four Varieties of Citrullus lanatus Seed Oils Cultivated in Côte d’Ivoire

Anne Marie Niangoran N’guetta, Yolande Dogoré Digbeu, Siaka Binate, Jean Parfait Eugène N’guessan Kouadio, Soumaïla Dabonné & Edmond Ahipo Dué · Biotechnology Journal International · 2014

Fatty Acid Composition and Physicochemical Properties of Four Varieties of Citrullus lanatus Seed Oils Cultivated in Côte d’Ivoire In this study, the physicochemical properties and fatty acids composition of four varieties of Citrullus lanatus seed oils cultivated in Côte d’Ivoir...

Open access Research Article 10.9734/BBJ/2015/12371

Identification of a Biochemical Indicator in Plants for Heavy Metal Contamination

Sonali Hazra Das, Mahasweta Paul, Shankha Ghosh Dastidar, Tulika Das & Srabanti Basu · Biotechnology Journal International · 2014

Aims: To identify a biochemical parameter in a plant(s) that can serve as an indicator of heavy metal pollution. Study Design: Design of the study includes collection of plant samples from a contaminated site and a rural site and to check their biochemical parameters related to o...

Open access Research Article 10.9734/BBJ/2015/14303

Sequence Homology Studies of Phospholipase A2-like Gene from Bloodstream form of Trypanosoma brucei

Ishaya Y. Longdet, Hajiya M. Inuwa, Isma’il A. Umar & Andrew J. Nok · Biotechnology Journal International · 2014

Aim: This work focused on the sequence homology studies of the enzyme, phospholipase A2  (PLA2), in Trypanosoma brucei  obtained from the blood of bull  in Federe, Plateau State, Nigeria, West Africa Place and Duration of Study: Department of Biochemistry, University of Jos, Nige...

Open access Research Article 10.9734/BBJ/2015/13971

Improvement of Regeneration of Pelargonium radula via Somatic Embryogenesis

A. R. Zuraida, M. A. Mohd Shukri, M. N. Erny Sabrina & O. Ayu Nazreena · Biotechnology Journal International · 2014

In vitro stem segments of Pelargonium radula cultured for callusing then differentiated into somatic embryos and subsequently regenerated plantlets. Initiation of callus was observed in culture medium containing low concentrations of the plant growth regulators, 2,4-dichloropheno...

Open access Research Article 10.9734/BBJ/2015/15337

Selenium Treatment Alleviated Oxidative Alteration Generated by Cadmium in Sunflower Roots

S. Issam, D. Wahbi & C. Yacine · Biotechnology Journal International · 2014

Selenium Treatment Alleviated Oxidative Alteration Generated by Cadmium in Sunflower Roots The present study investigated the role of selenium (Se) in regulating cadmium (Cd)-induced oxidative stress in sunflower (Helianthus annuus) roots. Short-term exposure of plants to 20 µM C...

Open access Research Article 10.9734/BBJ/2015/14802

Purification and Characterization of α-Glucan Phosphorylase Isoform Pho 2 from Spinach Leaves

Ritu Jain & Anil Kumar · Biotechnology Journal International · 2014

α-Glucan phosphorylase is an important enzyme of carbohydrate metabolism. In spinach leaves, it has been reported in two multiple forms viz. Pho 2 (cytosolic) and Pho 1 (plastidial). Here, we extracted and purified Pho 2 form of α-glucan phosphorylase from spinach using salting o...

Open access Research Article 10.9734/BBJ/2015/15106

Production and Partial Characterization of Pectinase from Snail (Archachatina marginata) Gut Isolates

E. C. Egwim & T. Caleb · Biotechnology Journal International · 2014

Aim: To identify isolates for industrial production of pectinase. Methodology: The isolates were screened for pectinolytic activity using pectin as substrate. Enzyme activity was expressed as mg of glucose equivalent released per ml of crude enzyme solution per second. The kineti...

Open access Research Article 10.9734/BBJ/2015/11449

Microsatellite DNA Marker Analysis Revealed Low Levels of Genetic Variability in the Wild and Captive Populations of Cirrhinus cirrhosus (Hamilton) (Cyprinidae: Cypriniformes)

Md. Abul Hasanat, Md. Fazlul Awal Mollah & Md. Samsul Alam · Biotechnology Journal International · 2014

Microsatellite DNA Marker Analysis Revealed Low Levels of Genetic Variability in the Wild and Captive Populations of Cirrhinus cirrhosus (Hamilton) (Cyprinidae: Cypriniformes) Aims: To reveal the genetic variability of wild and captive populations of the Indian major carp, mrigal...

Open access Research Article 10.9734/BBJ/2015/14895

Identification of Suitable Condition for Mannanase Production by Bacillus sp. GA2(1)

Sudathip Chantorn, Suda Natrchalayuth, Kulwadee Phannachet & Jirawan Apiraksakorn · Biotechnology Journal International · 2014

Identification of Suitable Condition for Mannanase Production by Bacillus sp. GA2(1) Aims: The effects of 1% (w/v) supplementation of additional 5 agricultural wastes, corn cob, bagasses, coffee residues, soybean meal, and copra meal for mannanase production by Bacillus sp.GA2(1)...

Open access Research Article 10.9734/BBJ/2015/15121

Evaluation of Hypoglycemic Efficacy of Methanolic Extracts of Moringa Oleifera and Phyllanthus amarus in Diabetic Rats

I. Oluwole Oyewole, A. Olufunke Taiwo & O. Nurudeen Quadri · Biotechnology Journal International · 2014

Evaluation of Hypoglycemic Efficacy of Methanolic Extracts of Moringa Oleifera and Phyllanthus amarus in Diabetic Rats Aims: This study was undertaken to determine the hypoglycemic efficacy of methanolic extracts of Moringa oleifera and Phyllanthus amarus in alloxan-induced diabe...

Open access Research Article 10.9734/BBJ/2015/13164

In vitro Antitheilerial Activity of Paluther (Artemether 80) Against Theileria lestoquardi

Hayat M. Farah, Tigani H. Elamin, Hassan E. Khalid, Abdel Rahim M. El. Hussein, Zakia A. Mohammed & Husna M. El Basheir · Biotechnology Journal International · 2014

In vitro Antitheilerial Activity of Paluther (Artemether 80) Against Theileria lestoquardi Aim: The aim of this study was to screen Artemether 80 for activity against Theileria lestoquardi (Apicomplexa: Theileridae) using buparvaquone as a standard drug. Study Design: In vitro st...

Open access Research Article 10.9734/BBJ/2015/10605

Comparative Studies on Some Edible Oils for Biodiesel Production in Nigeria

Humphrey Ibifubara, Nsikan I. Obot & Michael A. C. Chendo · Biotechnology Journal International · 2014

With the growing population and subsequent increasing demand for energy in Nigeria, coupled with the under-developed electrical energy platforms and the vast problems of the convectional energy sources such as continuous gas flaring, energy crisis is still lingering and enormous....

Open access Research Article 10.9734/BBJ/2015/14333

Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo

Othman E. Othman, Mohamed F. Abdel-Samad, Nadia A. Abo El-Maaty & Karima M. Sewify · Biotechnology Journal International · 2014

Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo Aim: The hypothalamic hormone, growth hormone-releasing hormone, is the principal stimulator of pituitary growth hormone (GH) synthesis and secretion. GHRH and its r...

Open access Research Article 10.9734/BBJ/2015/11619