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Kinetic and Thermodynamic Characterization of Latent and Activated Polyphenol Oxidase from Zrèzrou Yam (Dioscorea cayenensis rotundata) Cultivated in Ivory Coast for the Control of Enzymatic Browning

Judicael Kouamé, Sophie Nadège Gnangui, Mankambou Jacques Gnanwa & Lucien Patrice Kouamé · Biotechnology Journal International · 2026

In the Zrèzrou yam (Dioscorea cayenensis rotundata), cultivated in Côte d’Ivoire, polyphenol oxidase (PPO), the enzyme responsible for enzymatic browning, exists in latent and activated forms, whose properties were poorly documented. This study aims to conduct a detailed comparis...

Open access Research Article 10.9734/bji/2026/v30i2857

MicroRNA-Mediated Regulatory Mechanisms in Rice: Implications for Tissue Culture and Trait Improvement

V. Nivetha, Gibence H. Rose Winnie & Malavika M. Pillai · Biotechnology Journal International · 2026

MicroRNAs (miRNAs), which are diminutive, endogenously produced, non-coding RNA entities, function as critical regulators of gene expression by engaging in post-transcriptional control pathways. In rice (Oryza sativa), miRNAs have become crucial molecular components in numerous p...

Open access Research Article 10.9734/bji/2026/v30i2856

Expression Analysis of a Bilin-binding Protein Gene in Relation to Green and Yellow Larval Coloration in Antheraea pernyi

Yanxin Bai, Dong Yan, Qiuyu Xu, Kexing Gu & Qun Li · Biotechnology Journal International · 2026

Background: The larval color patterns of the wild silkworm Antheraea pernyi (Lepidoptera: Saturniidae) exhibit high diversity. However, the molecular basis underlying larval pigmentation in this species remains poorly understood and little pigment-related genes has been character...

Open access Research Article 10.9734/bji/2026/v30i2854

Estimation of Insulin Resistance in Non-Diabetic Female Patients by the TyG Index

Hayder Shareef Yaseen, Elaf Hussein Jihad & Jihad Hussein Jihad Alhamzawi · Biotechnology Journal International · 2026

Background: The development of metabolic and cardiovascular conditions is fundamentally driven by "insulin resistance" (IR). For effective preventive healthcare, it is essential to detect asymptomatic IR early in people without diabetes. Therefore, the primary objective of this i...

Open access Research Article 10.9734/bji/2026/v30i2855

Efficacy of Methyl Jasmonate and Calliette in the Management of Black Spot Disease of Pineapple (Ananas comosus) Caused by Fusarium moniliforme var. Subglutinans

Yeo Navigué Abou, Tehua Amoa Armist & Anguiby Bossombra Laurent Apollinaire · Biotechnology Journal International · 2026

Pineapples play an important role in the lives of people. However, they are threatened by black spot disease caused by Fusarium moniliforme var. subglutinans, which destroys 30% of production. Unfortunately, chemical methods of controlling this disease have proven ineffective due...

Open access Research Article 10.9734/bji/2026/v30i2853

Characterization of Partially Purified Trypsin and Chymotrypsin Inhibitor of Vigna pilosa

Anil S. Khandagale, Shekhar D. Tidke & Laxmikant H. Kamble · Biotechnology Journal International · 2026

Trypsin inhibitors are often associated with their negative effect as an antinutritional factor, but it is now known that they are beneficial for human health due to their anticarcinogenic, anti-inflammatory, and antiulcer activities. Vigna pilosa is a crop plant belonging to the...

Open access Research Article 10.9734/bji/2026/v30i2852

Developing Monotherapy against Hepatitis E Virus by Combining Phytochemicals Using Viro-Bioinformatics

Sanvedana Kamble, Preeti Mate, Pratiksha Bhoi & Sharddha Ranpise · Biotechnology Journal International · 2026

Hepatitis E virus (HEV) is a prominent cause of acute viral hepatitis worldwide, particularly threatening pregnant women and immunocompromised individuals due to the absence of specific antiviral treatments. This research employs an integrative in silico approach to identify phyt...

Open access Research Article 10.9734/bji/2026/v30i2848

Optimized in vitro Micropropagation Protocol for Acorus calamus L. Using Rhizome Explants

Swaranjali Gadhe, Chaitanya Mhaske & Shraddha Ranpise · Biotechnology Journal International · 2026

The present investigation has been conducted on “in-vitro micro propagation of Acoruscalamus L. using rhizome explants”. This study was undertaken to identify asuitable media combinations for the micro propagation of Acorus calamus L. The authenticated mother plant of Acorus cala...

Open access Research Article 10.9734/bji/2026/v30i2849

Combating Sarcopenia in Older Adults: The Synergistic Roles of Protein Intake and Exercise

Shravani Kawathekar & Gayatri Patil · Biotechnology Journal International · 2026

Given its correlation with frailty, disability, and death, sarcopenia—the age-related loss of muscle mass, strength, and functional ability—represents a significant public health concern. According to available data, older individuals' muscle loss is hastened by inadequate protei...

Open access Research Article 10.9734/bji/2026/v30i2850

Green Synthesis of Copper Nanoparticles Using Nyctanthes Arbor-tristis and Study of Its Biomedical Applications

Snehal Kulkarni, Neeraj Lokhande & Akshay Kulkarni · Biotechnology Journal International · 2026

Aim: The aim of the present study was to synthesize copper nanoparticles using aqueous extract of leaves of medicinal plant Nyctanthes arbor-tristis using green and ecofriendly method, and to study its bio medicinal application. Place and Duration of the Study: The study was carr...

Open access Research Article 10.9734/bji/2026/v30i2845

Exploring Keratin-Degrading Microbes in Poultry Soil: Isolation, Identification, and Feather Degradation Potential

Purnima Kabra, Shruti Kshirsagar, Tejaswini Patil & Akanksha Gaikwad. · Biotechnology Journal International · 2026

Keratin is an insoluble and protein-rich epidermal material found in feathers, hair, wool and skin but it poses a challenge in environmental waste management because of its resistance to degradation. Particularly, keratinolytic bacteria and fungi, have demonstrated the ability to...

Open access Research Article 10.9734/bji/2026/v30i2844

Antifungal, Antibacterial, Insecticidal and Nematicidal in vitro Activity of Four Plant-based Biopesticides

Mbang Gaston Elock, Abah Barakielle Arckange Bindzi, Arouna Meshuneke, Damien Fabrice Wassom, Onomo Pierre Effa & Cécile Annie Ewane · Biotechnology Journal International · 2026

Biopesticides are natural products used in agriculture to reduce the incidence or to eliminate crop pests. They represent an ecological and sustainable alternative to synthetic pesticides, the excessive use of which has serious impacts on human health and the environment. This wo...

Open access Research Article 10.9734/bji/2026/v30i2843

Recent Advances and Applications of Compost in Soil Health and Crop Productivity: A Comprehensive Review

Aparna Gunjal · Biotechnology Journal International · 2026

Composting is a biological process of the breakdown of complex matter into simple matter. The present review describes in detail the various applications of compost viz., effect of compost on soil properties; reduction in bulk density; compost to improve soil biological propertie...

Open access Research Article 10.9734/bji/2026/v30i2842

Reclaiming Roots: The Role of Indigenous Ecological Knowledge in Climate Adaptation and Biodiversity Conservation

Sonali Bhosale · Biotechnology Journal International · 2026

Indigenous Ecological Knowledge (IEK) represents a sophisticated, adaptive, and locally embedded way of understanding ecological systems, developed over millennia of direct interaction between humans and their environments. This knowledge encompasses long-standing observations, p...

Open access Research Article 10.9734/bji/2026/v30i2841

Anticancer and Antibacterial Activities of Polysaccharides Production from Radish (Raphanus sativus) and Lettuce (Lactuca sativa) Seeds in vitro

Sorial A. Moharib · Biotechnology Journal International · 2026

The present study was designed for investigate anticancer and antibacterial activities of the isolated polysaccharides from Radish (Raphanus sativus) and lettuce (Lactuca sativa) seeds (RPS and LPS respectively) against 4 carcinoma cell lines (HCT-116, HEPG2, HELA and MCF7).and 4...

Open access Research Article 10.9734/bji/2026/v30i1836