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Domain Analysis and Isolation of Coniferyl Alcohol Dehydrogenase Gene from Pseudomonas nitroreducens Jin1

Praveen P. Balgir & Dinesh Kalra · Biotechnology Journal International · 2014

Aim: The Aim of present study is to analyse conserved functional Short Dehydrogenase        Reductase (SDR) domain from bacteria. Based on the domain analysis selection of coniferyl alcohol dehydrogenase gene for isolation from Pseudomonas nitroreducens Jin1. Place and Duration o...

Open access Research Article 10.9734/BBJ/2014/7877

Integration of Reticuloendotheliosis Virus in Most of Tanzanian Fowl Pox Virus Isolates is not attributed to Imported Commercial Fowl Pox Vaccines

S. N. Masola, A. Mzula, C. J. Kasanga & P. N. Wambura · Biotechnology Journal International · 2014

Integration of Reticuloendotheliosis Virus in Most of Tanzanian Fowl Pox Virus Isolates is not attributed to Imported Commercial Fowl Pox Vaccines Aim: To investigate integration of reticuloendotheliosis virus (REV) in the Tanzanian fowlpox virus (FWPV) field isolates, and the im...

Open access Research Article 10.9734/BBJ/2014/10703

Isolation and 16S rRNA-Based Identification of Benomyl-Degrading Bacteria

Adil A. El-Hussein, Randa H. Elsalahi, Awad G. Osman, Ashraf M. Sherif & Marmar A. El Siddig · Biotechnology Journal International · 2014

Laboratory experiments were conducted to isolate and identify Benlate- (Benomyl) degrading microorganisms from two soil types collected from different locations in Khartoum State, Sudan. Benomyl degradation was studied at two temperatures (28 and 40ºC) in soil treated with three...

Open access Research Article 10.9734/BBJ/2014/10633

Histopathological, Hemochromatotic, Hypercholesterolemic, and Androgenic Effects of Escravos Crude oil on the Testis in Male Chinchilla Rabbits

J. O. Okoye, T. Ude, S. N. Ibekailo & C. J. Awalu · Biotechnology Journal International · 2014

Histopathological, Hemochromatotic, Hypercholesterolemic, and Androgenic Effects of Escravos Crude oil on the Testis in Male Chinchilla Rabbits Aim: This study aimed to investigate the effects of Escravos crude oil on serum concentrations of testosterone, total cholesterol, body...

Open access Research Article 10.9734/BBJ/2014/10808

Effects of External Carbon Source Concentration on Sulphate Removal by Selected Bacterial and Fungal species

O. B. Akpor, S. O. Dahunsi & R. Aransiola · Biotechnology Journal International · 2014

The aim of this study was to investigate the role of initial external carbon source concentration on sulphate removal by four bacterial and four fungal isolates under shake flask conditions. The test wastewater was filtered and supplemented with sodium acetate as the external car...

Open access Research Article 10.9734/BBJ/2014/10664

Allelopathic Effect of Cell-Free Medium of Microcystis aeruginosa Kützing on the Chromosomal Changes in Allium cepa Root Tips and Plumule Formation of Zea mays Seedling

M. M. EL-Sheekh, H. M. Khairy & R. EL-Shenody · Biotechnology Journal International · 2014

This work confirm the allelopathic toxic effect of both cell-free medium in log and death phase of growth of Microcystis aeruginosa on chromosomal changes of Allium cepa root tips and the plumule formation of Zea mays seedlings. The results showed high significant inhibition of m...

Open access Research Article 10.9734/BBJ/2014/8414

Molecular Identification of Cassava Mosaic Begomoviruses Associated with Cassava Mosaic Disease in the DR-Congo Using Primer Pairs

Marcel Muengula-Manyi, Claude Bragard, Stephan Winter, Adrien Kalonji-Mbuyi, Kabwe K. C. Nkongolo & Djim Tshilenge-Kanana · Biotechnology Journal International · 2014

Molecular analysis of cassava leaf samples from different regions of the Democratic Republic of Congo (DR-Congo) was conducted to identify cassava mosaic begomoviruses (CMBs)  associated with cassava infections in farmers' fields in Gandajika (Eastern Kasai), Bas-Congo and Kinsha...

Open access Research Article 10.9734/BBJ/2014/8112

Study of Genetic Fidelity of Somatic Embryos - Derived Plants of Gentiana kurroo Royle from Western Himalayas - Plant of Great Medicinal Value

Anshu Sharma, Rajinder Kaur & Ravinder Raina · Biotechnology Journal International · 2014

Aim: To study the effect of plant growth regulators on direct and indirect somatic embryogenesis on Gentiana kurroo Royle – an endangered medicinal plant. Place and Duration of Study: Department of Biotechnology, Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Sol...

Open access Research Article 10.9734/BBJ/2014/8653

Molecular Identification of Biodiversity of Fusarium species Isolated from Wilted Oil Palm and Date Palm in Nigeria

N. I. Chidi, A. A. Adekunle, E. I. Eziashi, I. B. Omamor, E. E. Odigie & J. Osagie · Biotechnology Journal International · 2014

Biodiversity of sixteen Fusarium isolates, isolated from the roots of oil palm and date palm in Nigeria was studied. Ten Fusarium isolates out of the sixteen were obtained from the oil palm while six isolates were from date palm. Random Amplified Polymorphic DNA (RAPD) was used t...

Open access Research Article 10.9734/BBJ/2014/3473

Molecular Relatedness and Morpho-Agronomic Characteristics of Congolese Accessions of Cassava (Manihot esculenta Crantz) for Breeding Purposes

G. Mamba-Mbayi, K. K. Nkongolo, R. Narendrula, P. Tshilenge Djim & A. Kalonji-Mbuyi · Biotechnology Journal International · 2014

Cassava serves as primary staple food of millions of people in the tropics and subtropics, and is used as a carbohydrate source in animal feed. Knowledge of agro-morphological characteristics and genetic relatedness is essential for an efficient recombination of varieties in a br...

Open access Research Article 10.9734/BBJ/2014/7423

Stability Evaluation and Degradation Kinetics of Ascorbic Acid in Baobab Fruit Pulp Formulated with the Seed Oil

Addai- Mensah Donkor, Matthew Tei, Jennifer Suurbaar, Abdallah Yakubu & Daniel Addae · Biotechnology Journal International · 2014

Aims: Baobab (Adansonia digitata) fruit pulp and the seed oil contain appreciable amount of vitamins and nutrients which help fight off diseases and afford commendable source of nourishment. It is essential to screen for the stability of the high vitamin C contents and validate t...

Open access Research Article 10.9734/BBJ/2014/9837

Evaluation of Halobacterial Extracellular Hydrolytic Activities in Several Natural Saline and Hypersaline Lakes from Romania

Ioan Păceşilă, Roxana Cojoc & Mădălin Enache · Biotechnology Journal International · 2014

This work presents the evaluation of extracellular hydrolytic activities involved in the degrading of organic compounds from several natural salt lakes located in the southern part of Romania. The paper approaches the extracellular enzymatic decomposition activity in accordance w...

Open access Research Article 10.9734/BBJ/2014/10239

The Effect of Colchicine on Adventitious Shoot Regeneration from Cultured Leaf Explants of Petunia hybrida

Hassan Abu-Qaoud & Munqez J. Y. Shtaya · Biotechnology Journal International · 2014

Aims: The effect of various concentrations of colchicine on adventitious shoot regeneration of two Petunia hybrid cultivars was studied. Study Design: All collected data were analyzed using the SAS software. One-way analysis of variance of the treatments was conducted for each ex...

Open access Research Article 10.9734/BBJ/2014/8465

Studies on Calli Mediated Multiple Shoot Regeneration from Rumex vesicarius L.

Lavanya Kakarla, Chakravarthy Rama, Mahendran Botlagunta & M. S. R Krishna · Biotechnology Journal International · 2014

Rumex vesicarius  L  is  a  valuable  potent  medicinal  herb,  which  belongs  to  family Polygonaceae. It possesses antimicrobial, anti- inflammatory, antidiarrhoeal and antioxidant properties. An attempt to study the In vitro callus induction and regeneration of plantlets from...

Open access Research Article 10.9734/BBJ/2014/8375