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All published articles 911

Respiratory Burst Enzymes and Oxidant-antioxidant Status in Nigerian Children with Sickle Cell Disease

Ayodele Adelakun, Olumide Ajani, Temitope Ogunleye, Elizabeth Disu, Ayokulehin Kosoko & Ganiyu Arinola · Biotechnology Journal International · 2014

Aim: To measure respiratory burst enzymes, pro-oxidants, antioxidants and red cell indices in Nigerian children with sickle cell disease (HbSS) below five years of age and compared with apparently healthy children with normal haemoglobin (HbAA). Method: A total of 45 subjects wer...

Open access Research Article 10.9734/BBJ/2014/7411

Chemical and Amino Acid Composition of Raw and Defatted African Mango (Irvingia gabonensis) Kernel

Ogungbenle Henry Niyi · Biotechnology Journal International · 2014

The proximate, mineral, functional properties, anti-nutrients and amino acid compositions of African mango (Irvingia gabonensis) seeds have been investigated. The results showed that seeds contained ash (2.49 g/100g), moisture (5.79 g/100g), protein (10.64 g/100g), fat (54.67 g/1...

Open access Research Article 10.9734/BBJ/2014/7609

Influence of Type and Age of Primary Somatic Embryo on Secondary and Cyclic Somatic Embryogenesis of Cassava (Manihot esculenta Crantz)

Jelili T. Opabode, Olufemi O. Oyelakin, Oluyemisi A. Akinyemiju & Ivan L. Ingelbrecht · Biotechnology Journal International · 2014

This study investigated the influence of age of the cotyledons, cut from primary somatic embryos (PSE) developed from shoot meristems (SM) or immature leaf lobes (LL), on secondary somatic (SSE) and cyclic (CSE) embryogenesis of two cassava cultivars at the Central Biotech Labora...

Open access Research Article 10.9734/BBJ/2014/3624

Prevalence and Antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus in Tertiary Care Hospitals

N. Kaur, R. Prasad & A. Varma · Biotechnology Journal International · 2014

Aims: Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in hospitals with hospital based outbreaks world-wide. The present study was carried out to estimate the prevalence of MRSA isolates in clinical specimens and to investigate the sensitivity pa...

Open access Research Article 10.9734/BBJ/2014/4245

Ficus religiosa L.: Callus, Suspension Culture and Lectin Activity in Fruits and In vitro Regenerated Tissues

Varsha Parasharami, Priya Yadav, Shilpa Mandkulkar & Sushama Gaikwad · Biotechnology Journal International · 2014

Ficus religiosa L. is a tree of immense cultural heritage in Asian countries. It is respected by followers of many religions and faiths. Fruits of Ficus religiosa L. are the ‘figs’ and possess many medicinal properties reported in ethnomedicinal and pharmacological studies. These...

Open access Research Article 10.9734/BBJ/2014/7338

Structural Analysis and Docking of Stilbene Synthase Protein from Chinese Grape Vine Vitis pseudoreticulata

K. Divya, G. Venkata Ramana & K. V. Chaitanya · Biotechnology Journal International · 2014

Aims: The present work aims to perform the molecular modeling of stilbene synthase protein from Chinese grape vine Vitis pseudoreticulata. Place and Duration of Study: The study has been performed in the Department of Biotechnology, GITAM Institute of Technology, GITAM University...

Open access Research Article 10.9734/BBJ/2014/3835

Exploration of Actinobacteria from Mangrove Ecosystems of Nizampatnam and Coringa for Antimicrobial Compounds and Industrial Enzymes

Usha Kiranmayi Mangamuri, Muvva Vijayalakshmi & Sudhakar Poda · Biotechnology Journal International · 2014

Aim: A study was made to examine the kinship between the seasonal distribution of actinobacteria and the physico-chemical properties of the mangrove sediments of Nizampatnam and Coringa located along the South East coast of Andhra Pradesh, India. Place and Duration of Study: Depa...

Open access Research Article 10.9734/BBJ/2014/6424

In vitro Propagation of Caralluma diffusa (Wight) N.E.Br

K. Kalimuthu, K. Kalaiyarasi, R. Prabakaran & T. Sasikala · Biotechnology Journal International · 2013

An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma diffusa, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant. The physiological effects of growth regul...

Open access Research Article 10.9734/BBJ/2014/7488

Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush)

Balwinder Singh · Biotechnology Journal International · 2013

The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon (Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract fo...

Open access Research Article 10.9734/BBJ/2014/6241

Agrobacterium-Mediated Transfоrmation of Maize with Antisense Suppression of the Proline Dehydrogenase Gene by an In Planta Method

Y. M. Moiseeva, V. A. Velikov, I. V. Volokhina, Yu. S. Gusev, O. S. Yakovleva & M. I. Chumakov · Biotechnology Journal International · 2013

Aims: The aim of this study was to estimate the efficiency of T-DNA transfer during Agrobacterium-mediated transformation of maize (Zea mays L.) at different temperatures. In addition, the way of T-DNA transfer was studied after application of an Agrobacterium suspension at maize...

Open access Research Article 10.9734/BBJ/2014/6504

Heavy Metals Concentration in Fish Mugil cephalus from Machilipatnam Coast and Possible Health Risks to Fish Consumers

P. V. Krishna, K. Madhusudhana Rao, V. Swaruparani & D. Srinivas Rao · Biotechnology Journal International · 2013

Heavy metals are dangerous to aquatic organisms and it can be bioaccumulated in the food chain leading to diseases in humans. Cumulative effects of metals or chronic poisoning may occur as a result of long term expore even to low concentrations. The accumulation of heavy metals c...

Open access Research Article 10.9734/BBJ/2014/4317

3D Structure Modeling of Human Telomere Repeat Binding Factor 2 and DNA-Protein Docking Studies

Koel Mukherjee, Dev Mani Pandey & Ambarish Saran Vidyarthi · Biotechnology Journal International · 2013

Aims: Human telomere repeat binding factor (hTRF2) is a double stranded telomere binding protein that plays key role in protecting the chromosome ends and a necessary building block of telomere structure maintenance. The aim of the present study was to focus on the modeling of 3D...

Open access Research Article 10.9734/BBJ/2014/6381

In vitro Propagation and GC-MS Studies of Ocimum basilicum Linn. var. pilosum (Willd.) Benth

Gaddaguti Venu Gopal, Srideepthi Repalle, Venkateswara Rao Talluri, Srinivasa Reddy Ronda & Prasada Rao Allu · Biotechnology Journal International · 2013

An in vitro propagation method is outlined for Ocimum basilicum Linn. var. pilosum (Willd.) Benth., a wild aromatic plant belongs to Lamiaceae family. Shoot buds were used as source of explants on MS media supplemented with different concentrations of growth regulators for callus...

Open access Research Article 10.9734/BBJ/2014/6225