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All published articles 911

Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix

Jagdish Singh & Manpreet Kaur · Biotechnology Journal International · 2013

Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH, matrix size, enzyme concentration, contact time and temper...

Open access Research Article 10.9734/BBJ/2014/6772

Establishment of an Efficient and Practical Virus-free Seedling Supply System by Means of Culture of Shoot Apexes, RT-PCR and Clonal Propagation in Sweet Potato (Ipomoea batatas)

Lanzhuang Chen, Chenti Xu, Zhaosheng Du, Takuro Hamaguchi, Toru Sugita, Ryutarou Nagata & Liming Guan · Biotechnology Journal International · 2013

Aims: Sweet potato (Ipomoea batatas) cv. “Miyazakibeni” was used as material for shoot apex culture, reverse transcription-polymerase chain reaction (RT-PCR) and clonal propagation to establish an efficient and practical virus-free seedling supply system in production of vegetati...

Open access Research Article 10.9734/BBJ/2014/6235

Different Methods for DNA Extraction from Yeast-Candida famata Isolated from Toddy

T. Santra, S. K. Ghosh & A. Chakravarty · Biotechnology Journal International · 2013

Aims: Isolation and biochemical characterization of yeasts from toddy and standardization of best method for DNA extraction from yeast. Study Design: Biochemical characterization of yeast and genomic DNA extraction by manual and kits methods. Place and Duration of Study: Departme...

Open access Research Article 10.9734/BBJ/2014/4884

Screening of Filamentous Fungi for Xylanases and Cellulases Not Inhibited by Xylose and Glucose

L. F. C. Ribeiro, L. F. Ribeiro, J. A. Jorge & M. L. T. M. Polizeli · Biotechnology Journal International · 2013

Aims: Screening different filamentous fungi for thermostable xylanases and cellulases that would not be inhibited by xylose and glucose, respectively. Methodology: Samples of fungi collected in the Atlantic forest region, Minas Gerais, Brazil, and some fungi from our Culture Coll...

Open access Research Article 10.9734/BBJ/2014/6066

Identification of Genes Putatively Involved in the Biosynthesis of Antitubercular Peptide in Streptomyces ribosidificus NRRL B-11466

Khaled M. Aboshanab, Nisreen M. Okba, Tarek S. El-banna & Ahmed A. Abd El-Aziz · Biotechnology Journal International · 2013

Aims: To determine the potential antitubercular activity of Streptomyces ribosidificus NRRL B-11466 both on genotypic and phenotypic levels.  Methodology: Standard methods and software programs were used for nucleotide/protein sequence analysis and phenotypic detection of antitub...

Open access Research Article 10.9734/BBJ/2014/5210

Activity of β-Amylase in Some Fungi Strains Isolated from Forest Soil in South-Western Nigeria

O. A. Oseni & M. M. Ekperigin · Biotechnology Journal International · 2013

Aims: The aim of this study was to isolate fungi species from Omo natural forest soil in Ogun State of Nigeria and study the amylases from the fungi species which are digestive enzymes that hydrolyze glycosidic bonds in starch to glucose, maltose, maltotriose and dextrin; and in...

Open access Research Article 10.9734/BBJ/2014/4686

Morphological Anomalies in Somatic Embryo Structure in Catharanthus roseus: Improving Embryo Germination by Amending Plant Growth Regulators, Activated Charcoal and Sucrose Level

Dipti ., Samar Fatima & Abdul Mujib · Biotechnology Journal International · 2013

The present study describes the incidence of somatic embryo (SE) irregularities in Catharanthus roseus (L.) G. Don. In this ornamental and anti-cancerous medicinal plant, SEs were developed at a very high rate from hypocotyl callus on MS medium, amended with  1.0 mg l-1 naphthale...

Open access Research Article 10.9734/BBJ/2014/4809

Effects of Utilization of Crushed, Boiled and Fermented Roselle Seeds (Hibiscus sabdariffa) on the Performance of Broiler Chickens

Maikano Mohammed Ari, Danlami Moses Ogah, Idris Danladi Hassan, Ibrahim Suleiman Musa-Azara, Nuhu Dalami Yusuf & Samuel Emmanuel Alu · Biotechnology Journal International · 2013

Aim of Study: The study examines the effects of utilization of crushed (CRRS), boiled (HTRS) and fermented (FRS) Roselle seeds (Hibiscus sabdariffa) on the performance of broiler chickens Study Design: A total of 135 Anak day-old broiler chicks were randomly assigned to three (3)...

Open access Research Article 10.9734/BBJ/2014/6061

Histopathological Effects of Glyphosate and Its Toxicity to the Earthworm Nsukkadrilus mbae

Oluah Ndubuisi Stanley & Ochulor Amarachi Joy · Biotechnology Journal International · 2013

This study was carried out to investigate the toxicity of Roundup, aglyphosate-containing herbicide to the earthworm Nsukkadrilus mbae and the associated histological changes. One hundred and sixty five earthworms were randomly divided into five groups and exposed to different co...

Open access Research Article 10.9734/BBJ/2014/6727

Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode

Sunita Premnath Koli & Hosakatte Niranjana Murthy · Biotechnology Journal International · 2013

Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2,...

Open access Research Article 10.9734/BBJ/2013/5417

L-Phenylalanine and L-Tyrosine Catabolism by Thermophilic Geobacillus stearothermophilus

M. Afzal, S. Al-Awadhi & S. Oommen · Biotechnology Journal International · 2013

Aims: This study investigated the potential of soil thermophile Geobacillus stearothermophilus for the biotransformation of phenylalanine and tyrosine. Study Design: G. Stearothermopilus grows well at 65ºC and has a good potential for transformation and biodegradation of many com...

Open access Research Article 10.9734/BBJ/2013/4516

Genetic Characterization of Insulin Growth Factor-1 and Its Receptor Genes in Egyptian Buffalo (Bubalus bubalis L.)

Othman E. Othman, Mohamed F. Abdel-Samad, Nadia A. Abo El Maaty & Karima M. Sewify · Biotechnology Journal International · 2013

Aim: The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA....

Open access Research Article 10.9734/BBJ/2013/4869

Characterization of Dairy Effluents by Physicochemical Parameters

Vishakha Sukhadev Shivsharan, Minal Wani & M. B. Khetmalas · Biotechnology Journal International · 2013

Aims: The dairy industry faces growing scrutiny of its environmental stewardship.  The potential impact of an individual operation on the environment varies with animal concentration, weather, terrain, soils, and numerous other conditions. It is hoped that management practices fo...

Open access Research Article 10.9734/BBJ/2013/3640

Improved Sample Preparation for PCR-Based Assays in the Detection of Xanthomonads Causing Bacterial Leaf Spot of Tomato

E. R. Mbega, J. Adriko, C. N. Mortensen, E. G. Wulff, O. S. Lund & R. B. Mabagala · Biotechnology Journal International · 2013

Aims: To develop a sampling procedure for PCR-based screening of bacterial leaf spot (BLS)-causing xanthomonads without DNA extraction from infected tomato plants. Place and Duration of Study: University of Copenhagen, Denmark and Sokoine University of Agriculture, Morogoro, Tanz...

Open access Research Article 10.9734/BBJ/2013/3810