Skip to content
Research Article Open access CC BY 4.0

A Sequential Injection Analysis Method for the Determination of Glyphosate and Aminomethylphosphonic Acid in Water Samples

Britta Jahnke, Carsten Frank, José F. Fernández, Bernd Niemeyer

Chemical Science International Journal · pp. 163–173 · Published 6 Nov 2014

10.9734/ACSJ/2015/13089

Abstract

A conventional laboratory method for the determination of glyphosate and its degradation product aminomethylphosphonic acid (AMPA) is high performance liquid chromatography (HPLC) followed by post column derivatization with o-phthaldialdehyde (OPA). However, AMPA is partly decomposed in the process causing a deviation in the AMPA detection or even making a simultaneous detection of glyphosate and AMPA with the described postcolumn procedure impossible. We used a compact sequential injection analysis system and optimized the process conditions for both analytes independently from each other. The process conditions were adjusted to the different chemical characteristics of AMPA (primary amine) and glyphosate (secondary amine), which needs to be oxidized to a primary amine prior to the derivatization. An ion exchange column was included in the system to eliminate amines interfering with the method. Limits of detection of 16 and 9 µg L-1 for glyphosate and AMPA, respectively are similar to those achieved with HPLC methods reported in literature, but compared to conventional HPLC methods only small amounts of reagents are consumed.

Glyphosate roundup AMPA sequential injection analysis SIA phosphororganic analysis

References (22)

  1. 1 Mobility and leaching of glyphosate: a review [DOI]
  2. 2 Prepubertal exposure to commercial formulation of the herbicide glyphosate alters testosterone levels and testicular morphology [DOI]
  3. 3 European eel (Anguilla anguilla) genotoxic and pro-oxidant responses following short-term exposure to Roundup(R)--a glyphosate-based herbicide [DOI]
  4. 4 Direct aqueous determination of glyphosate and related compounds by liquid chromatography/tandem mass spectrometry using reversed-phase and weak anion-exchange mixed-mode column [DOI]
  5. 5 Genotoxic effects of the herbicide Roundup® in the fish Corydoras paleatus (Jenyns 1842) after short-term, environmentally low concentration exposure [DOI]
  6. 6 Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment [DOI]
  7. 7 Development and validation of a liquid chromatography–fluorescence–mass spectrometry method to measure glyphosate and aminomethylphosphonic acid in rat plasma [DOI]
  8. 8 Dynamic supported liquid membrane tip extraction of glyphosate and aminomethylphosphonic acid followed by capillary electrophoresis with contactless conductivity detection [DOI]
  9. 9 Novel approach for the simultaneous analysis of glyphosate and its metabolites [DOI]
  10. 10 Rapid and direct determination of glyphosate and aminomethylphosphonic acid in water using anion-exchange chromatography with coulometric detection [DOI]
  11. 11 Determination of Glyphosate and Aminomethylphosphonic Acid in Water by LC Using a New Labeling Reagent, 4-Methoxybenzenesulfonyl Fluoride [DOI]
  12. 12 The herbicide glyphosate and its metabolite AMPA in the Lavaux vineyard area, western Switzerland: Proof of widespread export to surface waters. Part II: The role of infiltration and surface runoff [DOI]
  13. 13 A Fast Sequential Injection Analysis System for the Simultaneous Determination of Ammonia and Phosphate [DOI]
  14. 14 Developing a fluorimetric sequential injection methodology to study adsorption/desorption of glyphosate on soil and sediment samples [DOI]
  15. 15 Glossary of analytical terms [DOI]
  16. 16 Using Sequential Injection Analysis to Improve System and Data Reliability of Online Methods: Determination of Ammonium and Phosphate in Coastal Waters [DOI]
  17. 17 Determination of glyphosate mono-isopropylamine salt in process samples using flow injection analysis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection [DOI]
  18. 18 Determination of Glyphosate in Water Samples by Multi-pumping Flow System Coupled to a Liquid Waveguide Capillary Cell [DOI]
  19. 19 A sequential-injection reversed-phase chromatography method for fluorimetric determination of glyphosate and aminomethylphosphonic acid [DOI]
  20. 20 Liquid Chromatographic Determination of Glyphosate and Aminomethylphosphonic Acid (AMPA) in Environmental Water: Collaborative Study [DOI]
  21. 21 Determination of the herbicide glyphosate and its major metabolite aminomethylphosphonic acid by high-performance liquid chromatography after fluorescence labelling [DOI]
  22. 22 Determination of Glyphosate and Its Major Metabolite Aminomethylphosphonic Acid (AMPA) by High-Performance Liquid Chromatography after Derivatization with p-Toluenesulfonyl Chloride.

Cited by 4

Glyphosate and aminomethylphosphonic acid (AMPA) residues in Brazilian honey

Ana Paula Ferreira de Souza, Nadia Regina Rodrigues, Felix Guillermo Reyes Reyes · Food Additives & Contaminants: Part B · 2020

Showing 1 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

21

Page views

0

PDF downloads

0

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.