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Antibacterial Potential of Aqueous Neem Leaf Extract (Azadirachta indica A. Juss) on Spermatozoa Quality in Extended Porcine Semen

O. D. Ilori, O. A. Shokunbi, F. Alaba, S. Ajani, D. Omobayo

Asian Journal of Research in Animal and Veterinary Sciences · pp. 160–167 · Published 2 Aug 2018

10.9734/AJRAVS/2018/42167

Abstract

Aim: The present study was investigated to assess the antibacterial potential of aqueous neem leaves extracts (ANLE) on spermatozoa quality in extended porcine semen. Materials and Methods: Fresh semen was collected from a mature and intact boar (age, breed, body condition score, health status) using the glove-hand technique. The collected semen samples were diluted and allotted to six treatments with three replicates per treatment in a completely randomized design and evaluated at 0, 24 and 48 h of refrigeration at 17°C. Semen quality parameters such as progressive motility (%), viability (%), morphology (%), pH, acrosome integrity (%), and bacteria load (×104 CFU/mL) were evaluated. Results and Discussion: At 48 h, a significant difference (p < 0.05) in motility was observed across the treatments with Treatment 1 giving the highest mean value (84.67±2.40) and Treatment 6 (100% ANLE) gave the least value (70.00±0.00). At 48 h, there was a significant difference (p < 0.05) in viability across the treatments. Treatment 2 and Treatment 3 (25% ANLE) though with a significant difference between the means has given the closest mean value (79.67±0.33 and 76.67±0.67 respectively) to Treatment 1 (80.00±0.00). At 48 h, a significant difference (p < 0.05) in morphology was also observed across the treatments. Treatment 2 and Treatment 3 (25% ANLE), though with a significant difference between the means has given the closest mean values (81.33±0.67 and 79.33±0.33 respectively) to Treatment 1 (82.00±1.00). At 48 h, a significant difference (p < 0.05) in acrosome integrity, as well as pH, was also observed across the treatments. The general trend for bacteria load is a decline as the level of ANLE increases across the treatments at 48 h of refrigeration.  Conclusion: These findings suggest that 25% of ANLE  can be used in boar semen extension up to 48 h of storage at 17°C.

Artificial insemination aqueous neem leaf extract bacterial load semen quality

References (10)

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Cited by 3

Antioxidative Potential of Aqueous Neem Bark Extract (Azadirachta indica A. Juss) on Spermatozoa Quality in Extended Porcine Semen

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Antibacterial activities of aqueous neem bark extract (Azadirachta indica A. Juss) on spermatozoa quality in extended porcine semen

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