Skip to content
Research Article Open access CC BY 4.0

Antimicrobial Activities of Polysaccharides Isolated from Some Plant Seeds

Sorial A. Moharib, Rimon S. Adly

Biotechnology Journal International · pp. 34–47 · Published 21 Feb 2024

10.9734/bji/2024/v28i1712

Abstract

The present investigation is about the measurement of antimicrobial activity (AMA) of polysaccharides isolated from the seeds of pumpkin (Cucurbita pepo), purslane (Portulaca oleracea), safflower (Carthamu stinctorius),coriander (Coriandrum sativum) and rapeseed (Brassica napus) against Staphylococcus aureus (S.aureus), Escherichia coli (E.coli), Bacillus subtilis (B.subtilis) and (MRSA) at different concentrations using agar diffusion method In vitro. Polysaccharides (PSs) were extracted, isolated and purified from different seeds separately. Paper chromatographic analysis of the purified PSs, revealed the presence of different percentages of monosaccharides constituents per each seed PS. In vitro study was performed to evaluate the obtained PSs activities against 4 microbial strains (S. aureus, E. coli, B subtilis and MRSA) using agar diffusion method. PSs showed AMA against S. aureus and E. coli more than that of B subtilis and MRSA at 10% concentration and inhibition zones were estimated. Minimal inhibitory concentrations (MIC) of PSs against S. aureus, E. coli, B subtilis and MRS were found in the range of 1-5 mg/ml. The result demonstrates the killing effect of PSs against S. aureus, E. coli, B subtilis and MRSA at 10% PS within 24 hours. Inhibition zone diameters exhibited different levels of decreases with the PSs concentrations decreases against S. aureus, E. coli, B. subtilis and MRSA. Inhibition effects of PSs against different microbial strains were found to be depending on PSs concentration. However, the lowest concentration of PSs produced lower inhibitory activity against S. aureus B. subtilis E. coli and MRSA. The results obtained with all PSs showed best AMA against S. aureus and E. coli than the other two microbial strains used in the present study. Results indicated these PSs have AMA against Gram-positive and Gram-negative bacterium at 10% concentration. The common PSs consider important sources of antimicrobial agents with minimal inhibitory concentrations of 100-500µg/ml for treatment some infectious diseases.

Antimicrobial anticancer polysaccharides microorganisms In vitro

References (43)

  1. 1 Antibacterial resistance worldwide: causes, challenges and responses [DOI]
  2. 2 Natural products: A continuing source of novel drug leads [DOI]
  3. 3 A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria [DOI]
  4. 4 The Biology of Micro-Organisms [DOI]
  5. 5 Natural products to drugs: natural product-derived compounds in clinical trials [DOI]
  6. 6 The magnitude of the global problem of diarrhoeal disease: a ten-year update.
  7. 7 Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.) [DOI]
  8. 8 Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing. (an edible mushroom).
  9. 9 Antimicrobial natural products: an update on future antibioticdrug candidates [DOI]
  10. 10 Resistance in gram-negative bacteria: Enterobacteriaceae [DOI]
  11. 11 Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. [DOI]
  12. 12 Medicinal and biological potential of pumpkin: an updated review [DOI]
  13. 13 Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1 [DOI]
  14. 14 Carbon Source Requirements for Exopolysaccharide Production by Lactobacillus casei CG11 and Partial Structure Analysis of the Polymer [DOI]
  15. 15 Effect of Fucoidan on Disease Resistance of Black Tiger Shrimp [DOI]
  16. 16 Raphanus sativus (Radish): Their Chemistry and Biology [DOI]
  17. 17 Isolation, chemical investigation and antiviral activity of polysaccharides from Gracilaria corticata (Gracilariaceae, Rhodophyta) [DOI]
  18. 18 Antibiotic resistance: the perfect storm [DOI]
  19. 19 Polysaccharides from Sargassum tenerrimum: Structural features, chemical modification and anti-viral activity [DOI]
  20. 20 Challenges and opportunities in polysaccharides research and technology: The EPNOE views for the next decade in the areas of materials, food and health care [DOI]
  21. 21 The need for new therapeutic agents: what is in the pipeline? [DOI]
  22. 22 Purification and characterization of an antitumor polysaccharide from Portulaca oleracea L. [DOI]
  23. 23 Antibacterial activity of selected ethnomedicinal plants from South India [DOI]
  24. 24 Antibacterial activity of a polysaccharide isolated from Artemisia argyi leaf against Staphylococcus aureus and mechanism investigation [DOI]
  25. 25 Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus [DOI]
  26. 26 Antiproliferative and differentiating effects of polysaccharide fraction from fu-ling (Poria cocos) on human leukemic U937 and HL-60 cells [DOI]
  27. 27 Pectic Polysaccharides from Panax ginseng as the Antirotavirus Principals in Ginseng [DOI]
  28. 28 Preliminary studies on the chemical characterization and antihyperlipidemic activity of polysaccharide from the brown alga Sargassum fusiforme [DOI]
  29. 29 Chrysophanol, an Antimicrobial Anthraquinone from the Root Extract of Colubrina greggii [DOI]
  30. 30 Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds [DOI]
  31. 31 Isolation, purification, immunological and anti-tumor activities of polysaccharides from Gymnema sylvestre [DOI]
  32. 32 Plant-Based Polysaccharides and their Health Functions [DOI]
  33. 33 Quantitative Determination of Sugars on Paper Chromatograms [DOI]
  34. 34 Antimicrobial Properties and Toxicity of Anthraquinones by Microcalorimetric Bioassay [DOI]
  35. 35 Effect of slime on adherence of Staphylococcus aureus isolated from bovine and ovine mastitis [DOI]
  36. 36 POLYSACCHARIDES FROM MULBERRY LEAF IN RELATION TO THEIR ANTIOXIDANT ACTIVITY AND ANTIBACTERIAL ABILITY [DOI]
  37. 37 Methicillin-Resistant Staphylococcus aureus Colonization and Its Association with Infection Among Infants Hospitalized in Neonatal Intensive Care Units [DOI]
  38. 38 Antibacterial Effect of Seed Extracts of Cardamom (Elettaria cardamomum) against Staphylococcus aureus and Proteus mirabilis
  39. 39 New antimicrobial activity from Korean radish seeds (Raphanus sativus L.)
  40. 40 Anticarcinogenic Effect of Raphanus sativus on 1, 2 Dimethylhydrazine (DMH) Induced Colon Cancer in Rats [DOI]
  41. 41 Effect of two polysaccharides on chemically-induced colorectal cancer in rats.
  42. 42 Nature of seeds polysaccharide obtained from medicinal plant of Wrightia tinctoria R.Br. (Roxb.).
  43. 43 Methods in Carbohydrate Chemistry [DOI]

Cited by 0

No indexed citations yet.

Article metrics

Real usage data collected on this platform.

15

Page views

0

PDF downloads

2

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.