Bioactive Metabolites of Nodule Associated Microbes for Enhanced Drought Tolerance and Biocontrol Control Activity in Horsegram

Abstract

In the present study, the potential bioactive compounds detected in the ethyl acetate microbial extract of root nodules of horsegram were determined using the gas chromatography-mass spectroscopy (GC-MS).  The bioactive metabolites of nodule associated microbes (NAM) revealed the existence of several soluble metabolites which includes phthalic acid, butyl hex-3-yl ester, 2,4-Di-tert-butylphenol,Dodecyl acrylate, pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro, 1-Nonadecene, octadecane, 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester, hexadecanoic acid, methyl ester, dibutyl phthalate, diisooctyl phthalate, 1-docosene, heptadecane, 9-hexyl, pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl), n-Tetracosanol-1, ergotaman-3',6',18-trione, 9,10-dihydro-12'-hydroxy-2'-methyl-5'(phenylmethyl)-, (5'à,10à), eicosanal, ethanol, 2-(9-octadecenyloxy), E-15-heptadecenal, octatriacontyl pentafluoropropionate, etc.,.  Endophytic bacteria produces bioactive substances that provides resistance against phytopathogens as well as nutrient solubilization  during prolonged drought periods  in order to overcome biotic and abiotic stresses. Several antibacterial, antifungal, antiviral and antioxidant properties were brought under spotlight to realise the beneficial aspects of nodule associated microbes of horsegram. Understanding the roles of metabolites would enrich the crop growth under stressed environment by promoting the eco-friendly agriculture practices.