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Biochemical Characteristics of Penicillium crustosum FP 11 Xylanase II and an Assessment of the Properties of Xylanases Produced by the Genus Penicillium

Jorge W. F. Bittencourt, Vanessa C. Arfelli, Jaina C. Lunkes, Carla L. D. Torre, Jose L. C. Silva, Alexandre Maller, Rita de Cássia G. Simão, Marina K. Kadowaki

Annual Research & Review in Biology · pp. 64–75 · Published 30 Jul 2020

10.9734/arrb/2020/v35i730248

Abstract

  Penicillium crustosum FP 11 produces two extracellular xylanase, which are designated xylanase I and II, and are induced by corn stover. In this work, xylanase II was purified 40-fold with a recovery yield of 9.2% using DEAE-Sephadex and Sephadex G-75 gel filtration, and the biochemical characteristics of the enzyme were compared with other xylanases produced by the genus Penicillium. Xylanase II exhibited a single band on SDS–PAGE, and had an apparent molecular mass of 28 kDa. The optimal temperature and pH of xylanase II activity were 50ºC and 5.5, respectively. Xylanase II had activities of 61, 53 and 55% in the presence of Mg2+, DTT and β-mercaptoethanol, respectively; however, the enzyme was strongly inhibited by 5 mM Cu2+, EDTA, and SDS. Hydrolysis of beechwood xylan released mainly xylose and short-chain xylo-oligosaccharides as final products. Thus, an assessment of the enzymatic properties of xylanase II showed that its biochemical characteristics are best suited for the saccharification of lignocellulosic biomass into fermentable sugars.

Penicillium crustosum FP 11 biochemical characterization purification xylo-oligosaccharides.

References (38)

  1. 1 Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 [DOI]
  2. 2 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [DOI]
  3. 3 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  4. 4 The Determination of Enzyme Dissociation Constants [DOI]
  5. 5 Hemicelluloses for fuel ethanol: A review [DOI]
  6. 6 Biotechnology of Microbial Xylanases: Enzymology, Molecular Biology, and Application [DOI]
  7. 7 Application of Enzymes in the Pulp and Paper Industry [DOI]
  8. 8 Microbial xylanases and their industrial application in pulp and paper biobleaching: a review [DOI]
  9. 9 Application of enzymes for textile fibres processing [DOI]
  10. 10 Xylanases and Their Applications in Baking Industry
  11. 11 Structural and functional properties of low molecular weight endo-1,4-β -xylanases [DOI]
  12. 12 Purification and Characterization of Two Extracellular Xylanases from Penicillium sclerotiorum: A Novel Acidophilic Xylanase [DOI]
  13. 13 A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, purification and characterization [DOI]
  14. 14 GH10 xylanase D from Penicillium funiculosum: biochemical studies and xylooligosaccharide production [DOI]
  15. 15 Increased production of cellulases and xylanases by Penicillium echinulatum S1M29 in batch and fed-batch culture [DOI]
  16. 16 Production, purification and characterization of a minor form of xylanase from Aspergillus versicolor [DOI]
  17. 17 Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp. CGMCC 1669 [DOI]
  18. 18 Xylanase and β-xylosidase from Penicillium janczewskii : Purification, characterization and hydrolysis of substrates [DOI]
  19. 19 Screening of filamentous fungi to produce xylanase and xylooligosaccharides in submerged and solid-state cultivations on rice husk, soybean hull, and spent malt as substrates [DOI]
  20. 20 A new acidophilic endo-β-1,4-xylanase from Penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity [DOI]
  21. 21 Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene [DOI]
  22. 22 Novel Cold-adaptive Penicillium Strain FS010 Secreting Thermo-labile Xylanase Isolated from Yellow Sea [DOI]
  23. 23 A novel thermoacidophilic family 10 xylanase from Penicillium pinophilum C1 [DOI]
  24. 24 Alkalistable endo-β-1,4-xylanase production from a newly isolated alkalitolerant Penicillium sp. SS1 using agro-residues [DOI]
  25. 25 Cloning and constitutive expression of His-tagged xylanase GH 11 from Penicillium occitanis Pol6 in Pichia pastoris X33: Purification and characterization [DOI]
  26. 26 Molecular Cloning and Heterologous Expression of an Acid-Stable Endoxylanase Gene from Penicillium oxalicum in Trichoderma reesei [DOI]
  27. 27 Partial purification and characterization of xylanase produced by Penicillium expansum [DOI]
  28. 28 -Fructofuranosidase production by Aspergillus versicolor isolated from Atlantic forest and grown on apple pomace [DOI]
  29. 29 Purification and Characterization of a Xylanase from the Newly Isolated Penicillium rolfsii c3-2(1) IBRL [DOI]
  30. 30 Purification and properties of an extracellular endo-1,4-β-xylanase from Penicillium citrinum and characterization of the encoding gene [DOI]
  31. 31 Purification, biochemical characterization, and structure of recombinant endo-1,4-β-xylanase XylE [DOI]
  32. 32 Improvement in the bleaching of kraft pulp with xylanase from Penicillium crustosum FP 11 isolated from the Atlantic forest [DOI]
  33. 33 Functional characterization of Penicillium occitanis Pol6 and Penicillium funiculosum GH11 xylanases [DOI]
  34. 34 Newly Isolated Penicillium ramulosum N1 Is Excellent for Producing Protease-Resistant Acidophilic Xylanase [DOI]
  35. 35 Cloning of Penicillium canescens Endo-1,4-β-xylanase Gene and Construction of Multicopy Strains [DOI]
  36. 36 Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications [DOI]
  37. 37 Characterization of the Wild-Type and Truncated Forms of a Neutral GH10 Xylanase from Coprinus cinereus: Roles of C-Terminal Basic Amino Acid-Rich Extension in Its SDS Resistance, Thermostability, and Activity [DOI]
  38. 38 PURIFICATION OF A XYLANASE FROM Penicillium crustosum AND ITS POTENTIAL USE IN CLARIFYING FRUIT JUICE [DOI]

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