Cannabidiol Oil and Prednisolone Treatment Altered Hematologic Indices, Serum Urea, Creatinine and Cellular Architecture of Kidney on Cadmium Induced Toxicity in Male Wistar Rats

Abstract

This study investigated the impact of CBD oil and prednisolone on hematologic indices, urea, and creatinine among cadmium-induced toxicity in male rats. Forty male rats weighing 150g to 200g were assigned into 8 groups (1-8) with five rats each. Group 1 served as control, Group 2-8 received 1mg/kg body weight of prednisolone; 1.5mg/kg bw of cadmium; 1mg/kg bw of prednisolone + 0.2mg/kg bw of CBD-oil; 0.2mg/kg bw of CBD-oil + 2mg/kg bw of cadmium; 3mg/kg bw of Pred + 2mg/kg of cadmium; 0.1mg/kg bw of CBD-oil and 0.2mg/kg bw of CBD-oil respectively. The administration was done using gavage for 14 days. The present results revealed that PCV, haemoglobin, and RBC count of the treated groups were decreased significantly (p<0.05) comparedto the control. TWBC count significantly increased in treated groups than in control. Platelet count significantly increased in groups treated with pred+ cd, CBD-oil (0.1ml), and CBD-oil (0.2ml) than control. Neutrophil count was significantly reduced in groups treated with CBD oil (0.1ml) than in control and other groups. Lymphocyte count significantly increased in groups treated with cadmium+ CBD oil and CBD oil (0.1ml) than in other groups. Eosinophil count significantly decreased in groups treated with prednisolone and pred +CBD oil than control. Monocyte count significantly increased in groups treated with cadmium, pred +cadmium, and CBD oil (0.1ml) than in control. There was a significant decrease in groups treated with pred +CBD-oil, cadmium + CBD-oil, and CBD-oil (0.2ml) than control. Serum urea significantly decreased in the group treated with pred + CBD oil than control. Serum creatinine significantly increased in groups treated with cadmium + CBD oil and CBD oil (0.2ml) compared to the control and prednisolone groups. Histology of the kidney revealed mesangial expansion, hypertrophy of renal corpuscle, hemorrhage, and lymphocyte infiltration in groups treated with pred+ CBD oil and CBD oil (0.1ml) compared to the control. We conclude that CBD oil, prednisolone, and cadmium administration at different doses induced biochemical alterations, altered hematologic indices, and cytoarchitecture of the kidney. Therefore, if these results apply to humans, combined use of CBD oil and prednisolone should be supplemented with blood tonics, especially in chronic kidney disease.