Skip to content
Research Article Open access CC BY 4.0

Cultivating Tomorrow: Harnessing Gene Editing and Silencing for Precision Horticulture

Shubham Jain, G. Ranganna, Priyanka Shivaji Jadhav, Ujjwal Shrivastava, Ankita Sood, Lalu Prasad, Suraj Luthra, Anushi

Journal of Advances in Biology & Biotechnology · pp. 147–160 · Published 9 Feb 2024

10.9734/jabb/2024/v27i1688

Abstract

Precision Horticulture, propelled by gene editing and silencing technologies, emerges as a transformative approach to address the demands of sustainable and efficient crop production. Utilizing CRISPR-Cas9 and RNA interference, horticulturists can precisely modify and regulate plant genomes, ushering in a new era of tailored crops. Gene editing enables the development of crops with heightened resistance to pests, diseases, and improved adaptability. This targeted enhancement, coupled with accelerated breeding processes, results in resilient varieties that meet the challenges of evolving agricultural landscapes. Concurrently, gene silencing technologies allow for the suppression of undesirable traits, extending the shelf life of produce and minimizing post-harvest losses. Integration of these technologies into Precision Horticulture not only optimizes crop traits but also promotes sustainability by reducing reliance on chemical inputs. The approach aligns with environmental conservation, ensuring a more ecologically balanced and resource-efficient cultivation. However, responsible deployment and ethical considerations are paramount for widespread acceptance, highlighting the need for a harmonious balance between technological innovation and ethical utilization in shaping the future of horticulture.

Gene editing development resistance targeted

References (57)

  1. 1 A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity [DOI]
  2. 2 Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [DOI]
  3. 3 Food Security: The Challenge of Feeding 9 Billion People [DOI]
  4. 4 Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [DOI]
  5. 5 Role for a bidentate ribonuclease in the initiation step of RNA interference [DOI]
  6. 6 A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants [DOI]
  7. 7 An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells [DOI]
  8. 8 Gene silencing by microRNAs: contributions of translational repression and mRNA decay [DOI]
  9. 9 Breeding Technologies to Increase Crop Production in a Changing World [DOI]
  10. 10 CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture [DOI]
  11. 11 Argonaute proteins: key players in RNA silencing [DOI]
  12. 12 Molecular Mechanisms of RNA Interference [DOI]
  13. 13 Single Processing Center Models for Human Dicer and Bacterial RNase III [DOI]
  14. 14 Revealing the world of RNA interference [DOI]
  15. 15 Methylation Protects miRNAs and siRNAs from a 3′-End Uridylation Activity in Arabidopsis [DOI]
  16. 16 A pathogen-inducible endogenous siRNA in plant immunity [DOI]
  17. 17 The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis [DOI]
  18. 18 Diverse Pathways Generate MicroRNA-like RNAs and Dicer-Independent Small Interfering RNAs in Fungi [DOI]
  19. 19 RNA interference: concept to reality in crop improvement [DOI]
  20. 20 In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans [DOI]
  21. 21 RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis [DOI]
  22. 22 RNA Silencing in Plants: Mechanisms, Technologies and Applications in Horticultural Crops [DOI]
  23. 23 Dicer-Independent Primal RNAs Trigger RNAi and Heterochromatin Formation [DOI]
  24. 24 Structural insights into mechanisms of the small RNA methyltransferase HEN1 [DOI]
  25. 25 A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis [DOI]
  26. 26 RNA-Mediated Silencing in Algae: Biological Roles and Tools for Analysis of Gene Function [DOI]
  27. 27 Small non-coding RNAs mount a silent revolution in gene expression [DOI]
  28. 28 RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators [DOI]
  29. 29 The status of RNAi-based transgenic research in plant nematology [DOI]
  30. 30 Tomato transgenic plants expressing hairpin construct of a nematode protease gene conferred enhanced resistance to root-knot nematodes [DOI]
  31. 31 DNA Repair in Mammalian Cells [DOI]
  32. 32 RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato [DOI]
  33. 33 RNA interference in designing transgenic crops [DOI]
  34. 34 RNAi mediated broad-spectrum transgenic resistance in Nicotiana benthamiana to chilli-infecting begomoviruses [DOI]
  35. 35 RNAi-mediated SMV P3 cistron silencing confers significantly enhanced resistance to multiple Potyvirus strains and isolates in transgenic soybean [DOI]
  36. 36 Small RNA warfare: exploring origins and function of trans-species microRNAs from the parasitic plant Cuscuta [DOI]
  37. 37 Downregulation of the lycopene ε-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum [DOI]
  38. 38 Plant MicroRNAs: Biogenesis, Functions, and Applications [DOI]
  39. 39 RNAi-mediated resistance to rice black-streaked dwarf virus in transgenic rice [DOI]
  40. 40 Transgenic Nicotiana benthamiana plants expressing a hairpin RNAi construct of a nematode Rs-cps gene exhibit enhanced resistance to Radopholus similis [DOI]
  41. 41 RNA Interference: A Promising Approach for Crop Improvement [DOI]
  42. 42 RNAi-mediated resistance to yellow mosaic viruses in soybean targeting coat protein gene [DOI]
  43. 43 Host-derived gene silencing of parasite fitness genes improves resistance to soybean cyst nematodes in stable transgenic soybean [DOI]
  44. 44 The Heterodera glycines effector Hg16B09 is required for nematode parasitism and suppresses plant defense response [DOI]
  45. 45 Coat protein 3 of Rice tungro spherical virus is the key target gene for development of RNAi mediated tungro disease resistance in rice [DOI]
  46. 46 RNA interference-mediated resistance to Tobacco streak virus in transgenic peanut [DOI]
  47. 47 RNA interference (RNAi): Application in crop improvement: A review [DOI]
  48. 48 Expression of Meloidogyne incognita PolA1 hairpin RNA reduced nematode multiplication in transgenic tomato [DOI]
  49. 49 RNA Interference A fine tuner of gene regulation: a Review [DOI]
  50. 50 Integrated annotations and analyses of small RNA-producing loci from 47 diverse plants [DOI]
  51. 51 Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans [DOI]
  52. 52 Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming [DOI]
  53. 53 RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture [DOI]
  54. 54 On the Role of Transposable Elements in the Regulation of Gene Expression and Subgenomic Interactions in Crop Genomes [DOI]
  55. 55 Conferring root-knot nematode resistance via host-delivered RNAi-mediated silencing of four Mi-msp genes in Arabidopsis [DOI]
  56. 56 Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance [DOI]
  57. 57 Engineering Plant Resistance to Tomato Yellow Leaf Curl Thailand Virus Using a Phloem-Specific Promoter Expressing Hairpin RNA [DOI]

Cited by 6

RNA Interference and Spray-Induced Gene Silencing (SIGS): A Next-Generation Tool in Horticultural Biotechnology

Nishtha Dixit, N. Kaushik, Maria Fe Andres · BIO Web of Conferences · 2026

Showing 1 of 6 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

53

Page views

0

PDF downloads

20

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.