Skip to content
Research Article Open access CC BY 3.0

Development and Validation of First Derivative Spectrophotometric Method for Quantification of Darunavir in Tablets

Josilene Chaves Ruela Corrêa, Cristina Helena dos Reis Serra, Hérida Regina Nunes Salgado

Journal of Pharmaceutical Research International · pp. 722–730 · Published 30 Jan 2014

10.9734/BJPR/2014/6326

Abstract

Aims: Darunavir is widely used in HIV/AIDS therapy. It is a HIV protease inhibitor that has excellent efficacy against the virus. The aim of this study is to develop and validate an analytical method fast and free of interferences for determination of darunavir ethanolate as raw material and tablet dosage form. Methodology: As the formulation excipients show high interference in darunavir determination by a direct UV absorption measurement a derivative spectrophotometry was applied. A selective, easy and fast method was achieved employing simple and cheap instrumentation by using first-order derivative spectrophotometry. Results: The first-derivation of spectrum of the drug measured between 200 and 400 nm allowed identification of the analyte and showed absence of placebo interference. The assay was based on the absorbance at 276nm. The linear concentration range was established from 11 to 21 μg/mL. The intra-day and inter-day precision expressed as RSD was 0.06% and 3.75% respectively with mean recovery of 99.84%. Conclusion: The proposed analytical method is able to quantify darunavir as raw material and tablets and can be used routinely by any laboratory applying a spectrophotometer with a derivative accessory. The great difference of the method proposed here is that it proves to be free of placebo interferences as well as simple, fast and low cost.

HIV/AIDS darunavir pharmaceutical dosage form analytical validation derivative spectrophotometry

References (14)

  1. 1 Validation of a rapid and sensitive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) assay for the simultaneous determination of existing and new antiretroviral compounds [DOI]
  2. 2 A HPLC–MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation [DOI]
  3. 3 Estratégias para aumento de sensibilidade em espectrofotometria UV-VIS [DOI]
  4. 4 Darunavir: A Critical Review of Its Properties, Use and Drug Interactions [DOI]
  5. 5 Efficacy, safety and pharmacokinetics of 900/100 mg of darunavir/ritonavir once daily in treatment-experienced patients [DOI]
  6. 6 HIV protease inhibitors: garlic supplements and first‐pass intestinal metabolism impact on the therapeutic efficacy [DOI]
  7. 7 A simple and sensitive HPTLC method for quantitative analysis of darunavir ethanolate tablets [DOI]
  8. 8 RP-HPLC separation and characterization of unknown impurities of a novel HIV-protease inhibitor Darunavir by ESI-MS and 2D NMR spectroscopy [DOI]
  9. 9 Stability Study of Darunavir Ethanolate Tablets Applying a New Stability-Indicating HPLC Method [DOI]
  10. 10 A Critical Review of Properties of Darunavir and Analytical Methods for Its Determination [DOI]
  11. 11 Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules [DOI]
  12. 12 HPTLC METHOD FOR DETERMINATION OF DARUNAVIR IN RAT PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES [DOI]
  13. 13 Desarrollo y validación de un método para la determinación de darunavir en plasma mediante LC-MS/MS [DOI]
  14. 14 Spectrophotometric Estimation of Darunavir in Bulk and Pharmaceutical Formulations

Cited by 6

Darunavir: A comprehensive profile

· Profiles of Drug Substances, Excipients and Related Methodology · 2021

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

Eliane Gandolpho Tótoli, Hérida Regina Nunes Salgado · Brazilian Journal of Pharmaceutical Sciences · 2015

Showing 2 of 6 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

30

Page views

0

PDF downloads

3

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.