Distribution of Methicillin-Resistant Staphylococcus aureus (MRSA) in Apparently Healthy Population and its Susceptibility to Saponins from Dialium guineense
W. O. Obonga, C. O. Nnadi, M. O. Agbo, F. C. Kenechukwu, U. Nwodo
Journal of Pharmaceutical Research International · pp. 2200–2209 · Published 25 Sep 2014
10.9734/BJPR/2014/12943Abstract
Aims: The ethanolic extracts of stem bark and fruit pulp as well as saponins from Dialium guineense were assayed for antibacterial activity against Gram positive and negative strains and clinical strains of methicilin resistant Staphylococcus aureus (MRSA) isolated from different locations on human body aged 20-30 years within the University of Nigeria community. Methodology: Agar diffusion technique was adopted. Results: The results showed that MRSA is predominant in apparently healthy population of the University community with 100% in males and 92.3% females showing positive case in nasal swab, 87.5% and 96.6 % positive from ear swabs of male and female volunteers respectively; and 77.7% positive from the high vaginal swabs of females. MRSA and other clinical isolates showed higher susceptibility to saponins compared to crude extracts; however, Bacillus cereus (NRRL 14724 and 14725) were not susceptible to the saponins from D. guineense. The MICs of the saponins were 31.25 mg/mL (B. subtilis ATCC 6051, P. aeruginosa, S. typhi, S. knitambo, P. mirabilis and S. aureus), 62.50 mg/mL (E. coli) and 125 mg/mL (P. aeruginosa ATCC 10145). Comparable MICs of higher values were obtained with the crude ethanolic extracts of stem bark and fruit pulp against MRSA and clinical isolates. Conclusion: The present findings revealed wide distribution of MRSA in an apparently healthy population in Nigeria and the susceptibility patterns showed the presence of a broad spectrum antibacterial agent in D. guineense.
References (30)
- 1 Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis
- 2 Antimicrobial agents and chemotherapy—1969 [DOI]
- 3 Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. [DOI]
- 4 Antimicrobial resistance: the example of Staphylococcus aureus [DOI]
- 5 SHEA Guideline for Preventing Nosocomial Transmission of Multidrug-Resistant Strains ofStaphylococcus aureusandEnterococcus [DOI]
- 6 Screening methods for natural products with antimicrobial activity: A review of the literature [DOI]
- 7 Linezolid vs vancomycin: analysis of two double-blind studies of patients with methicillin-resistant Staphylococcus aureus nosocomial pneumonia.
- 8 Staphylococcus aureus Bacteremia [DOI]
- 9 Does antibiotic exposure increase the risk of methicillin-resistant Staphylococcus aureus (MRSA) isolation? A systematic review and meta-analysis [DOI]
- 10 Antimicrobial resistance: the example of Staphylococcus aureus [DOI]
- 11 Mechanisms of Antibiotic Resistance in Staphylococcus Aureus [DOI]
- 12 The importance of the development of antibiotic resistance in Staphylococcus aureus [DOI]
- 13 Genetics of Antimicrobial Resistance in Staphylococcus Aureus [DOI]
- 14 Effects of temperature and humidity on the efficacy of methicillin-resistantStaphylococcus aureuschallenged antimicrobial materials containing silver and copper [DOI]
- 15 In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria [DOI]
- 16 Broad-spectrum microbicidal activity, toxicologic assessment, and materials compatibility of a new generation of accelerated hydrogen peroxide-based environmental surface disinfectant [DOI]
- 17 Transcription and Translation Products of the Cytolysin Gene psm-mec on the Mobile Genetic Element SCCmec Regulate Staphylococcus aureus Virulence [DOI]
- 18 Inhibition of cell wall turnover and autolysis by vancomycin in a highly vancomycin-resistant mutant of Staphylococcus aureus [DOI]
- 19 Genesis of Methicillin‐ResistantStaphylococcus aureus(MRSA), How Treatment of MRSA Infections Has Selected for Vancomycin‐ResistantEnterococcus faecium,and the Importance of Antibiotic Management and Infection Control [DOI]
- 20 Bactericidal activity of antiseptics against methicillin-resistant Staphylococcus aureus [DOI]
- 21 Prevalence of and Risk Factors for Community-Acquired Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Colonization in Children Seen in a Practice-Based Research Network [DOI]
- 22 Emergence of Quinolone Resistance amongst Escherichia coli strains isolated from Clinical infections in some Lagos State Hospitals, in Nigeria. [DOI]
- 23 Managing CA-MRSA infections: Current and emerging options
- 24 The role of vancomycin in the persistence or recurrence of Staphylococcus aureus bacteraemia [DOI]
- 25 Antibacterial and Antifungal Activities of Isatin N-Mannich Bases [DOI]
- 26 Comparison of microbiological and clinical characteristics based on SCCmec typing in patients with community-onset meticillin-resistant Staphylococcus aureus (MRSA) bacteraemia [DOI]
- 27 Prevalence of and Risk Factors for Community-Acquired Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Colonization in Children Seen in a Practice-Based Research Network [DOI]
- 28 Teicoplanin pharmacokinetics in intravenous drug abusers being treated for bacterial endocarditis [DOI]
- 29 The treatment of staphylococcal infections with special reference to pharmacokinetic, pharmacodynamic and pharmacoeconomic considerations. [DOI]
- 30 Anti-Vibrio and preliminary phytochemical characteristics of crude methanolic extracts of the leaves of Dialium guineense (Wild) [DOI]
Cited by 9
Leonard E. Okonkwo, Charles O. Nnadi, Matthias O. Agbo · Tropical Journal of Phytochemistry and Pharmaceutical Sciences · 2024
Li Yumei, Li Yamei, Li Qiang · Journal of Nanomaterials · 2017
Showing 2 of 9 known citations — external sources report more than can currently be individually listed.
Related research
- Prevalence of Schistosomia haematobium and Staphylococcus aureus among Pregnant Women in Akure — shares topic coverage
- Carriage of Drug-Resistant Staphylococcus aureus in the Anterior Nares of a Healthy Student Population — shares topic coverage
- Two Endogenous Endophthalmitis Cases Accompanying Nephrological Condition in Diabetic Patients — shares topic coverage
- Antibiotic Susceptibility Patterns in Adenotonsillar Bacterial Isolates in a Tertiary Hospital in Sub-Saharan Africa — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
14
Page views
0
PDF downloads
0
Outbound clicks
9
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.