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Research Article Open access CC BY 4.0

Droplet Digital PCR – A Superior Complementary Technique for SARS-CoV-2 Detection

I. M. Hussaini, S. Gide, B. Musa, M. A. Sulaiman, A. Usman

Asian Journal of Biology · pp. 12–15 · Published 1 Aug 2020

10.9734/ajob/2020/v9i430092

Abstract

Accurate and timely SARS-CoV-2 detection in suspected persons is crucial in the fight against its spread. Many techniques have been developed to meet up with the continuously growing demand, however some of these techniques lack the required accuracy, sensitivity and specificity. The current reference standard technique for SARS-CoV-2 detection is RT-PCR, but studies have shown that false-negative results are inevitable and data can be non-reproducible when samples and primers are not appropriately verified and validated. Droplet digital PCR (ddPCR) is a newly introduced technique that performs precise nucleic acid quantification. Researchers have evaluated the efficacy of ddPCR and the technique has shown promising results even in specimens with low viral load. ddPCR has shown increased accuracy, precision, sensitivity and specificity. Furthermore, it is less affected by annealing and amplification inhibitors. This suggests that ddPCR can be used as a complementary detection technique especially in convalescent cases.

SARS-CoV-2 ddPCR RT-PCR nucleic acids viral load.

References (19)

  1. 1 High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number [DOI]
  2. 2 Absolute quantification by droplet digital PCR versus analog real-time PCR [DOI]
  3. 3 Real-time RT-PCR in COVID-19 detection: issues affecting the results [DOI]
  4. 4 Quantification using real-time PCR technology: applications and limitations [DOI]
  5. 5 Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data [DOI]
  6. 6 Recent advances and perspectives of nucleic acid detection for coronavirus [DOI]
  7. 7 Comparison of seven commercial RT-PCR diagnostic kits for COVID-19 [DOI]
  8. 8 Combination of RT‐qPCR testing and clinical features for diagnosis of COVID‐19 facilitates management of SARS‐CoV‐2 outbreak [DOI]
  9. 9 ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens [DOI]
  10. 10 Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool [DOI]
  11. 11 Applications of Digital PCR for Clinical Microbiology [DOI]
  12. 12 Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective [DOI]
  13. 13 Droplet Digital Polymerase Chain Reaction (PCR) Outperforms Real-Time PCR in the Detection of Environmental DNA from an Invasive Fish Species [DOI]
  14. 14 Covert coronavirus infections could be seeding new outbreaks [DOI]
  15. 15 Digital PCR provides sensitive and absolute calibration for high throughput sequencing [DOI]
  16. 16 Evaluation of Digital PCR for Absolute RNA Quantification [DOI]
  17. 17 A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer [DOI]
  18. 18 Digital droplet PCR (ddPCR) for the precise quantification of human T-lymphotropic virus 1 proviral loads in peripheral blood and cerebrospinal fluid of HAM/TSP patients and identification of viral mutations [DOI]
  19. 19 Comparison of reverse-transcriptase qPCR and droplet digital PCR for the quantification of dengue virus nucleic acid [DOI]

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