Effect of Different Priming Methods on Seed Viability and Seedling Growth of Upland Rice under Drought Stress
Muhammad Kadir, Baso Darwisah, Miss Rahma Yassin, Erna Halid, Syamsia ., Sri Muliani
Asian Journal of Agricultural and Horticultural Research · pp. 15–24 · Published 20 Feb 2023
10.9734/ajahr/2023/v10i2222Abstract
This study aims to determine the effect of different priming methods on the viability and early growth of upland rice under drought stress. Priming is an important approach to improve the resistance of upland rice plants to drought stress from the germination phase to growth, especially on sub-optimal land. There are several efficient priming methods as seed pretreatment to increase germination and tolerance to drought stress. An effective priming method is needed to increase the germination and growth of upland rice seedlings for application in dryland agriculture. Therefore, an experiment was conducted at the Seed laboratory of Pangkep State Polytechnic of Agriculture, Indonesia, from September to October of 2022 to investigate the effect of several priming methods on the germination and growth of upland rice seedlings under drought stress. The experiment consisted of 2 stages, germination testing and seedling growth phase testing with the addition of water stress treatment. Experiment 1 was conducted in a completely randomized design (CRD) with four replications, including Control (no priming), Osmopriming with 15% and 20% PEG solution, Redox Priming with 3% and 6% H2O2 solution, and Organic Priming using 50% and 75% Moringa leaf extract. Experiment 2 was conducted using a two-factor of factorial in Randomized Block design (RBD) with three replications. The first factor is the Seedling results of stage 1 experiments (selected the best of each priming method) includes 4 treatments each Control treatment, Osmopriming with 15% PEG solution, Redox Priming with 3% H2O2 solution, and Organic Priming with 50% Moringa Leaf Extract. The second factor is the level of Drought Stress conducted by 100%, 60%, and 30% of Field Capacity. The results showed that the priming method with Osmopriming 15% PEG solution gave the best results on Seed germination percentage (87.5%) followed by Organic priming with 50% Moringa Leaf Extract (SGP 85%). Under drought stress conditions with 30% field capacity, the highest increase in proline levels was observed with H2O2 redox priming 3% (10.3 µ-mol. g-1 ), while the average root growth of all primed seedlings showed better root growth than seeds without priming treatment. Seed priming gives better results on the growth and physiological activities of upland rice at several levels of drought stress, in the early growth phase of seedlings
References (25)
- 1 Proline accumulation in plants: a review [DOI]
- 2 Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging [DOI]
- 3 Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice [DOI]
- 4 Salt stress mitigation by seed priming with UV-C in lettuce plants: Growth, antioxidant activity and phenolic compounds [DOI]
- 5 A novel Cys2/His2 zinc finger protein gene from sweetpotato, IbZFP1, is involved in salt and drought tolerance in transgenic Arabidopsis [DOI]
- 6 Priming crops against biotic and abiotic stresses: MSB as a tool for studying mechanisms [DOI]
- 7 Seed Priming with Beta-Amino Butyric Acid Improves Abiotic Stress Tolerance in Rice Seedlings [DOI]
- 8 Seed Priming A Critical Review [DOI]
- 9 H2O2 seed priming improves tolerance to salinity; drought and their combined effect more than mannitol in Cakile maritima when compared to Eutrema salsugineum [DOI]
- 10 Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.) [DOI]
- 11 Seed priming upregulates antioxidant defense and glyoxalase systems to conferring simulated drought tolerance in wheat seedlings [DOI]
- 12 Effects of Seed Priming on Germination and Seedling Growth Under Water Stress in Rice [DOI]
- 13 Effects of External Potassium (K) Supply on Drought Tolerances of Two Contrasting Winter Wheat Cultivars [DOI]
- 14 Seed osmopriming improves plant growth, nodulation, chlorophyll fluorescence and nutrient uptake in alfalfa ( Medicago sativa L.) – rhizobia symbiosis under drought stress [DOI]
- 15 Seed Priming of Trifolium repens L. Improved Germination and Early Seedling Growth on Heavy Metal-Contaminated Soil [DOI]
- 16 Priming mediated stress and cross-stress tolerance in plants: Concepts and opportunities [DOI]
- 17 Stimulating Plant Tolerance Against Abiotic Stress Through Seed Priming [DOI]
- 18 Biotechnological interventions for inducing abiotic stress tolerance in crops [DOI]
- 19 Improving seed germination and seedling growth of Lepidium sativum with different priming methods under arsenic stress [DOI]
- 20 Seed priming with Mg(NO3)2 and ZnSO4 salts triggers physio-biochemical and antioxidant defense to induce water stress adaptation in wheat (Triticum aestivum L.) [DOI]
- 21 Hydrogen peroxide pre-treatment for seed enhancement in Cotton (Gossypim hirsutum L.) [DOI]
- 22 Study on Characteristics of Germination and Drought-resistance Index by Osmotic Stress in Rice
- 23 Study on drought resistance and screening of the drought resistance assessment indexes at germinating stage of rice
- 24 Evaluation of drought-tolerance in some tropical wheat genotypes (Triticum aestivum L.) at different osmotic-stress level [DOI]
- 25 International Seed Testing Association (ISTA) [DOI]
Cited by 11
Michaël Kenne Fomekong, Elise Fabiola Tchouake Tetang, Carine Nono Temegne · 2025
Tamara Sakhno, Mykola Marenych, Anatolii Semenov · Advances in Agronomy · 2025
A H I Nurrahma, H H Putri, L Nuraini · IOP Conference Series: Earth and Environmental Science · 2024
Michaël Kenne Fomekong, Elise Fabiola Tchouake Tetang, Carine Nono Temegne · Discover Plants · 2025
Muhammad Kadir, Muhammad Dzulkifly Ashan, Syamsia Syamsia · Journal of Agriculture · 2023
Mehmet Demir Kaya, Nurgül Ergin, Pınar Harmancı · OCL · 2024
Showing 6 of 11 known citations — external sources report more than can currently be individually listed.
Related research
- Infection of Aedes aegypti (Diptera: Culicidae) larvae and Adults by the Entomopathogenic Fungus Metarhizium anisopliae (Metschn.) Sorokin — shares topic coverage
- Comparative Efficacy of Microbial Biopriming on Germination Kinetics and Seedling Vigor in Rice (Oryza sativa L.) — shares topic coverage
- Quality Evaluation of Maize Akamu as Affected by Dehulling, Partial Germination and Oil Seed Supplementation — shares topic coverage
- Effect of Germination on the Physicochemical and Antinutritionnal Parameters of White Beans (Phaseolus vulgaris L.) Seeds Cultivated in Côte d'Ivoire — shares topic coverage
- Effect of Soaking and Germination on the Functional Properties of Kpaakpa (Hildegardia Barteri) Seed Flour Using Response Surface Methodology — shares topic coverage
Article metrics
Real usage data collected on this platform.
38
Page views
0
PDF downloads
16
Outbound clicks
11
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.