Etravirine Lipid Nanoparticles: Development and Validation Using Specific RP-HPLC Technique

Abstract

Introduction: A common, exact & economical reverse phase-high performance liquid chromatographic approach for coinciding measurement of anti-retroviral medicine, Etravirine in the formulation of nanoparticle has been devised. This approach is a revolutionary analytical methodology for detecting substance at the same time. Methods: Validation & optimization chromatographic conditions were completed as per the guidelines of ICH. The disconnection was done on a column measuring (150mm x 4.6mm, 5μ) using the mobile phase consisted of acetonitrile and 10mM ammonium acetate buffer in the ratio of 90:10 v/v of pH 2.7 as the mobile phase in the ratio 85:15 at a flow rate of 1ml/min for a short run time of 13 minutes. The wavelength was 310nm & the column temperature was kept at 26°C. Results: The devised technique was linear at 10, 20, 30, 40, as well as 50 g/ml, with a regression coefficient of 0.999 for each of these concentrations. For etravirine, the LOD readings were 0.514 g/ml, whereas the LOQ readings were 1.713 g/ml. Etravirine was shown to have a recovery rate of 98.8%. Conclusion: The approach was, precise, accurate & rapid with excellent inter- & intra-day accuracy. The approach was also useful for characterization & coinciding measurement of medicines in formulation of nanoparticles.