Skip to content
Research Article Open access CC BY 4.0

Evaluation of Cytotoxic Effects of Methanolic Extract and Fractions of Mirabilis jalapa (L.) Leaf

Sekinat Okikiola Azeez, Aanuoluwapo Nifemi Olowu, Anyim Godwin, Joseph Tosin Apata, Bolajoko Ayinke Akinpelu

Journal of Applied Life Sciences International · pp. 25–35 · Published 29 Oct 2021

10.9734/jalsi/2021/v24i630242

Abstract

This study examined the potential cytotoxicity of Mirabilis jalapa L. methanolic crude leaf extract and its fractions against brine shrimp nauplii (Artemia salina L.) and Allium cepa L. roots. The leaf extraction was done according to standard technique and crude extract was partitioned using n-hexane, water, ethyl acetate and butanol to obtain their respective fractions. Allium cepa root growth inhibition of M. jalapa methanolic crude extract and fractions were evaluated as well as brine shrimp lethality of the fractions based on standard methods. Also, phytochemical screening of the methanolic crude leaf extract was carried out according to standard methods. The result showed that M. jalapa methanolic crude leaf extract caused a significant reduction in cell mitotic index (32.96%) compared with the control (52.13%). The butanol fraction produced the highest mitotic inhibitory activity on A. cepa cell division at 0.3 mg/ml. Moreover, the butanol fraction produced the highest percentage lethality (LC50 1.45 μg/ml) against brine shrimp nauplii. There was a strong correlation between brine shrimp lethality and mitotic cell inhibition with butanol fraction as the most potent in both models. The methanolic leaf crude extract tested positive for alkaloids, cardiac glycosides, flavonoids, saponins, steroids, tanins and triterpenes. The methanolic crude extract of M. jalapa leaf and its fractions exhibited effective cytotoxic effect on A. cepa and brine shrimps. Butanol fraction, with the most cytotoxic activity among the tested extracts, demonstrates a promising source for novel anticancer agents.

Mirabilis jalapa cytotoxicity brine shrimps Allium cepa mitotic index

References (20)

  1. 1 Medicinal plants and traditional medicine in Africa
  2. 2 The Allium test as a standard in environmental monitoring [DOI]
  3. 3 The Use of Biological Assays to Evaluate Botanicals [DOI]
  4. 4 Allium Cepa Root Chromosomal Aberration Assay: A Review [DOI]
  5. 5 Chromosomal and Nuclear Alterations in Root Tip Cells of Allium Cepa L. Induced by Alprazolam [DOI]
  6. 6 Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants [DOI]
  7. 7 Medicinally Important Phytochemicals: An Untapped Research Avenue
  8. 8 Advances in Pharmaceutical Biotechnology [DOI]
  9. 9 EVALUATION OF ETHNOMEDICAL CLAIMS AND BRINE SHRIMP TOXICITY OF SOME PLANTS USED IN TANZANIA AS TRADITIONAL MEDICINES.
  10. 10 Pharmacognosy 6th Edition. [DOI]
  11. 11 Potential Genotoxic and Cytotoxic Effects of Plant Extracts [DOI]
  12. 12 Evaluation of Cytotoxic and Genotoxic Effects of Euphorbia Triaculeata Forssk. Extract [DOI]
  13. 13 Application of Phytochemicals in Pharmaceuticals [DOI]
  14. 14 5-Aminouracil and other inhibitors of DNA replication induce biphasic interphase–mitotic cells in apical root meristems of Allium cepa [DOI]
  15. 15 Phylogenetic Relationships within Nyctaginaceae Tribe Nyctagineae: Evidence from Nuclear and Chloroplast Genomes [DOI]
  16. 16 Indonesian Mirabilis jalapa Linn. : A Pharmacognostical and Preliminary Phytochemical Investigations [DOI]
  17. 17 Phytochemicals and Their Role in Pharmaceuticals [DOI]
  18. 18 Database on chromosome count of some Indian plants [DOI]
  19. 19 Evaluation of the bio-toxicity and cytogenetic effects of methanolic leaf extract of Heliotropium indicum L. [DOI]
  20. 20 Cytological studies on the Monochlamydeae from North India [DOI]

Cited by 5

Effects of Mirabilis jalapa L. root extract and sildenafil on paroxetine-induced sexual dysfunction in male rats and characterization of its phytoconstituents by UPLC-MS

Asad Ur Rahman, Fiaz Alam, Zia Ur Rehman · South African Journal of Botany · 2023

DEVELOPMENT OF ANAEMIA TREATMENT USING ETHANOL EXTRACT OF MIRABILIS JALAPA YELLOW FLOWERS

Fera Widyanti, Dono Indarto, Yuliana Heri Suselo · Media Penelitian dan Pengembangan Kesehatan · 2024

Showing 2 of 5 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

14

Page views

0

PDF downloads

1

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.