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Research Article Open access CC BY 4.0

Evaluation of Phytochemical, Antimicrobial Activities and Toxicological Analysis of Scent Leaf (Ocimum gratissimum L.) Leaf Extracts

M. Umar, C. P. Nkemelu, M. R. Sagir, S. Y. Mohammed, G. K. Ajiya, I. B. Mohammed, A. A. Yaya, A. A. Kigbu, S. A. Ojo, I. M. Abdulkarim, I. Y. Tafinta, I. C. Amuta

Asian Journal of Research in Medical and Pharmaceutical Sciences · pp. 1–11 · Published 18 Jul 2019

10.9734/ajrimps/2019/v7i330123

Abstract

Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of the leaves of scent leaf (Ocimum gratissimum L.) were investigated. Methods: All the analyses were carried out using standard scientific procedures using Soxhlet extraction, well-diffusion agar antimicrobial testing and in vivo acute toxicity testing. Results: The phytochemical analysis according to standard screening tests using conventional protocols revealed the presence of anthraquinone, saponins, tannins, terpenoids and alkaloids, which were detected in methanol extract analyzed. But, flavonoids, glycosides, phlobatannins and steroids were not detected in the methanol extracts analyzed. While only flavonoids were detected in chloroform extract. All other phytochemicals were absent. The extract fractions generally exhibited slight antibacterial activities on Staphylococcus aureus, Bacillus subtilis, Salmonella Typhi and Escherichia coli. But, the extracts showed no effect against Candida albicans. The minimum inhibitory concentration of O. gratissimum was determined with S. aureus and B. cereus recording MICs at the lowest concentration (12.5 mg/mL) of the methanol and chloroform extracts used. While methanol and chloroform extracts were found to have recorded moderate activity S. Typhi and E. coli at the MIC of 50mg/mL. The methanol and chloroform extract recorded MBC of 50 mg/mL on B. subtilis and S. Typhi. However, Salmonella Typhi was inhibited at the concentration of 100 mg/mL of chloroform scent leaf extract. The MICs of C. albicans were not determined in the methanol and chloroform scent leaf extracts analyzed. The in vivo toxicity of O. gratissimum extracts against albino rats revealed that the plant extracts were found to exhibit mild toxicity at higher doses, but the overall remark showed that the plant extract was safe at various concentrations. Conclusions: The plant can be used in the treatment of various diseases caused by the test microbes, and the plant has less toxicity when administered orally.

Ocimum gratissimum phytochemistry toxicology antimicrobial minimum inhibitory concentration

References (21)

  1. 1 Medicinal plants and traditional medicine in Africa
  2. 2 District Laboratory Practice in Tropical Countries [DOI]
  3. 3 Phytochemical screening and Extraction: A Review
  4. 4 District Laboratory Practice in Tropical Countries, Part 1
  5. 5 Efficacy of Aqueous and Methanol Extracts of Some Medicinal Plants for Potential Antibacterial Activity
  6. 6 Water plants ; a study of aquatic angiosperms [DOI]
  7. 7 Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities [DOI]
  8. 8 Antimicrobial activities of crude leaf extracts of Acalypha wilkesiana [DOI]
  9. 9 Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale
  10. 10 Underestimating the Toxicological Challenges Associated with the Use of Herbal Medicinal Products in Developing Countries [DOI]
  11. 11 Phytochemical Studies And Antioxidant Properties Of Four Medicinal Plants Used In Cameroon [DOI]
  12. 12 Alkaloid, tannin and saponin contents of some Nigerian medicinal plants
  13. 13 Investigation of Antioxidant/Oxidant Status and Antimicrobial Activities of Lentinus tigrinus [DOI]
  14. 14 Histopathological studies on the toxicity of Ocimum gratissimumleave extract on some organs of rabbit [DOI]
  15. 15 Antimicrobial activity of the ethanol extract and fractions of the seeds of Garcinia kola Heckel (Guttiferae) [DOI]
  16. 16 Phytochemical Analysis and Antimicrobial Effect of Lemon Grass (Cymbopogon citratus) Obtained From Zaria, Kaduna State, Nigeria [DOI]
  17. 17 Phytochemical analysis and acute toxicity tests of two medicinal plant extracts [DOI]
  18. 18 Evaluation of Phytochemical and in vitro Antimicrobial Effects of Solanum lycopersicum Linn. (Tomato) on Oral Thrush and Human Cariogenic Pathogens [DOI]
  19. 19 Antibacterial activity of leaf extracts of Ocimum sanctum L. against Xanthomonas campestris pv. mangiferaeindicae.
  20. 20 Acute Toxicity and Dose Fixation Studies on Chloroxylon swietenia Dc Bark Extracts on Streptozotocin Induced Diabetic Rats [DOI]
  21. 21 Internationale Pharmaceutica Sciencia [DOI]

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