Extended-Spectrum Beta-lactamase and Metallo-beta-lactamase Production among Escherichia coli and Klebsiella pneumoniae Strains from Urine of Pregnant Women in Afikpo, Ebonyi State, Nigeria
Olugbue Victor Uzochukwu, Okonkwo Evelyn Ijeoma, Ogundapo Solomon Segun, Odochi, Uchendu Damian, Nkama Onochie Jeff., Uchendu Damian Odochi, Eze Blessing Nwadimkpa, Segun, Ogundapo Solomon, Ijeoma, Okonkwo Evelyn, Jeff., Nkama Onochie
Biotechnology Journal International · pp. 35–45 · Published 5 Jul 2023
10.9734/bji/2023/v27i4689Abstract
Aim: The aim of this study was to determine the occurrence of Extended-spectrum beta-lactamase (ESBL) and Metallo-beta-lactamase (MBL) among Escherichia coli and Klebsiella pneumoniae strains from pregnant women attending Mater Misericordia Hospital Afikpo, Ebonyi state, Nigeria. Study Design: This is a laboratory based prospective study carried out on pregnant women suspected of having urinary tract infection and was requested to undergo diagnosis at microbiology laboratory of the hospital. Place and Duration of Study: The study was conducted in the Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic, Unwana, Afikpo, Ebonyi State, Nigeria from October, 2022 to January, 2023. Methodology: Clean-catch midstream urine samples were collected from 206 pregnant women suspected of having urinary tract infection and were requested to undergo medical diagnosis at microbiology laboratory of the hospital. The urine samples were processed following standard microbiological procedure. Antimicrobial susceptibility testing was determined using the disc diffusion method, while ESBL phenotypes were determine by the Double-Disc Synergy Test (DDST). Disc potentiation test was performed to check for MBL production. Results: Out of the 206 urine samples processed, 24 (11.7 %) E. coli and 12 (5.8 %) K. pneumoniae were isolated. The antimicrobial susceptibility of the isolates recorded a 100 % resistance with Amoxicillin/Clavulanic acid and Cotrimoxazole. The Gram-negative isolates showed a high sensitivity of 100 % to Netilmicin, Meropenem and Ofloxacin. Overall, 35 (97.2 %) multidrug resistance (MDR) was observed of the bacteria isolates. A total of 9 (37.5 %) E. coli and 4 (33.3 %) K. pneumoniae was found positive for ESBL production whereas, 5 (20.8 %) E. coli and 2 (16.7 %) K. pneumoniae were MBL positive. Conclusion: The level of drug resistance in this study underscores the need for regular surveillance for effective management of urinary tract infection in pregnancy.
References (36)
- 1 Performance standards for antimicrobial susceptibility testing
- 2 Performance standards for antimicrobial susceptibility testing
- 3 Carbapenem Resistance: A Review [DOI]
- 4 Annals of Clinical Microbiology and Antimicrobials
- 5 Extended spectrum beta-lactamase and metallo beta-lactamase production among Escherichia coli and Klebsiella pneumoniae isolated from different clinical samples in a tertiary care hospital in Kathmandu, Nepal [DOI]
- 6 Bacterial profile of urinary tract infection and antimicrobial susceptibility pattern among pregnant women attending at Antenatal Clinic in Dil Chora Referral Hospital, Dire Dawa, Eastern Ethiopia [DOI]
- 7 Emergence of high drug resistant bacterial isolates from patients with health care associated infections at Jimma University medical center: a cross sectional study [DOI]
- 8 Asymptomatic urinary tract infection among pregnant women attending the antenatal clinic of Hawassa Referral Hospital, Southern Ethiopia [DOI]
- 9 Urinary pathogenic bacterial profile, antibiogram of isolates and associated risk factors among pregnant women in Ambo town, Central Ethiopia: a cross-sectional study [DOI]
- 10 Epidemiology of antimicrobial resistance in Lebanese extra-hospital settings: An overview [DOI]
- 11 Urinary tract infection in pregnancy [DOI]
- 12 Urinary Tract Infections and Preeclampsia among Pregnant Women Attending Two Hospitals in Mwanza City, Tanzania: A 1:2 Matched Case-Control Study [DOI]
- 13 Bacterial profile, antibiotic susceptibility pattern and associated factors among pregnant women with Urinary Tract Infection in Goba and Sinana Woredas, Bale Zone, Southeast Ethiopia [DOI]
- 14 Asymptomatic bacteriuria among pregnant women attending antenatal care at Mbale Hospital, Eastern Uganda [DOI]
- 15 Prevalence and antimicrobial susceptibility pattern of urinary tract infection among pregnant women attending Hargeisa Group Hospital, Hargeisa, Somaliland [DOI]
- 16 Urinary Tract Infection among Pregnant Women at Pumwani Maternity Hospital, Nairobi, Kenya: Bacterial Etiologic Agents, Antimicrobial Susceptibility Profiles and Associated Risk Factors [DOI]
- 17 Urinary Tract Infection among Pregnant Women and its Associated Risk Factors: A Cross-Sectional Study [DOI]
- 18 Asymptomatic Bacteriuria, antimicrobial susceptibility pattern and associated risk factors among pregnant women attending antenatal care in Assosa General Hospital, Western Ethiopia [DOI]
- 19 Antibiotic Overconsumption in Pregnant Women With Urinary Tract Symptoms in Uganda [DOI]
- 20 Prevalence and Risk Factors of Urinary Tract Infection among Pregnant Women in Ismailia City, Egypt [DOI]
- 21 Extended spectrum beta-lactamase producing Uropathogens in Asymptomaticpregnant women attending antenatal care in an urban community secondary health facility [DOI]
- 22 Detection of Beta-Lactamases (ESBL and MBL) Producing Gram-Negative Pathogens in National Public Health Laboratory of Nepal [DOI]
- 23 Urinary Tract Infection in Pregnant Women at Kathmandu, Nepal [DOI]
- 24 Prevalence of Urinary Tract Infection among Pregnant Women in Kano, Northern Nigeria [DOI]
- 25 Prevalence of Urinary Tract Infections (UTI) Among Pregnant Women in University Of Benin Teaching Hospital (UBTH) Benin City, Nigeria [DOI]
- 26 Prevalence of urinary tract infection in antenatal period at tertiary care hospital in rural Bangalore [DOI]
- 27 DETECTION OF METALLO-?-LACTAMASESENCODING GENES AMONG CLINICAL ISOLATES OF ESCHERICHIA COLI IN A TERTIARY CARE HOSPITAL, MALAYSIA [DOI]
- 28 Bacterial Profile and ESBL Screening of Urinary Tract Infection Among Asymptomatic and Symptomatic Pregnant Women Attending Antenatal Care of Northeastern Ethiopia Region
- 29 Phenotypic detection of Extended Spectrum β-Lactamases (ESBL) among gram negative uropathogens reveals highly susceptibility to imipenem [DOI]
- 30 Phenotypic detection of metallo-beta-lactamase production in clinical isolates of Escherichia coli and Klebsiella pneumoniae in a tertiary care hospital [DOI]
- 31 Antibiotic resistance pattern of Pseudomonas aeruginosa with special reference to Imipenem and Metallo-beta lactamase Production
- 32 Antimicrobial resistance and ESBL profile of Uropathogens among pregnant women at Edna Adan Hospital, Hargeisa, Somaliland [DOI]
- 33 African Journal of Microbiology Research [DOI]
- 34 Antibiotics for asymptomatic bacteriuria in pregnancy [DOI]
- 35 Prevalence and Antimicrobial Susceptibility Pattern of Urinary Tract Infection among Pregnant Women Attending Hargeisa Group Hospital, Hargeisa, Somaliland [DOI]
- 36 Prevalence and Bacteriology of Symptomatic Urinary Tract Infection among Pregnant women at Mbarara Regional Referral Hospital, South-western Uganda [DOI]
Cited by 6
Mohd Afzal, Abhishek Sharma, Ashok Kumar Sah · Urological Science · 2025
Wael Hafez, Jalal Al Shareef, Afraa Alyoussef · International Journal of Maternal and Child Health and AIDS · 2024
Meseret Geremew, Destaw Kebede, Abebe Fenta · BMC Microbiology · 2026
Showing 3 of 6 known citations — external sources report more than can currently be individually listed.
Related research
- Antibacterial Activity of Bidens pilosa Extracts on Escherichia coli O157: H7 Isolated from Apparently Healthy Individuals — shares topic coverage
- Urine Analysis and Determination of Drug Sensitivity Pattern of Isolated Escherichia coli from Urinary Tract Infected Patients — shares topic coverage
- Evaluation of the Effects of Pediococcus acidilactici Isolated from Wara, a Nigerian Milk Product, in the Prevention of Diarrhea and the Modulation of Intestinal Microflora in Wistar Rats — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
- Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire — shares topic coverage
Article metrics
Real usage data collected on this platform.
53
Page views
0
PDF downloads
19
Outbound clicks
6
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.