Fast Detection of Chloramphenicol in Raw Milk Using a Hairpin Aptamer-templated Silver Nanoclusters
Hengchao Li, Bin Hu, Yuliang Cheng, Hang Yu, Yunfei Xie, Weirong Yao, Yahui Guo, He Qian
Asian Journal of Chemical Sciences · pp. 8–21 · Published 7 Apr 2021
10.9734/ajocs/2021/v9i419077Abstract
In this presented work, a facile and efficient method was established for the detection of chloramphenicol (CAP) based on target-induced structure transformation of aptamer. This aptamer DNA with a hairpin structure can coincidentally serve as a template for the synthesis of bright silver nanoclusters (quantum yield 16.36%). The binding of CAP with aptamer DNA could cause the destruction of the hairpin structure, resulting in the quenching of the fluorescence of silver nanoclusters (AgNCs). It costs less than 10 minutes to complete the assay, and excellent sensitivity was achieved with detection limit of 0.052 nmol/L. The selectivity and recovery experiments also demonstrated satisfactory results of this proposed protocol. The method has potential applicability, and provides a new strategy for the development of label-free sensors based on aptamer and AgNCs.
References (48)
- 1 Selection of single-stranded DNA molecules that bind and inhibit human thrombin [DOI]
- 2 Triggered amplification by hybridization chain reaction [DOI]
- 3 Programming biomolecular self-assembly pathways [DOI]
- 4 Quantum Dot−Aptamer Conjugates for Synchronous Cancer Imaging, Therapy, and Sensing of Drug Delivery Based on Bi-Fluorescence Resonance Energy Transfer [DOI]
- 5 Atomically precise metal nanoclusters: stable sizes and optical properties [DOI]
- 6 Strand displacement amplification—an isothermal, in vitro DNA amplification technique [DOI]
- 7 Specific interactions between silver(i) ions and cytosine–cytosine pairs in DNA duplexes [DOI]
- 8 Co-selection of multi-antibiotic resistance in bacterial pathogens in metal and microplastic contaminated environments: An emerging health threat [DOI]
- 9 Quantum-Dot/Aptamer-Based Ultrasensitive Multi-Analyte Electrochemical Biosensor [DOI]
- 10 Ag Nanocluster Formation Using a Cytosine Oligonucleotide Template [DOI]
- 11 DNA-Based Hybridization Chain Reaction for Amplified Bioelectronic Signal and Ultrasensitive Detection of Proteins [DOI]
- 12 Adsorption behavior of the antibiotic levofloxacin on microplastics in the presence of different heavy metals in an aqueous solution [DOI]
- 13 Evolutionary consequences of antibiotic use for the resistome, mobilome and microbial pangenome [DOI]
- 14 Graphene Oxide-Based Amplified Fluorescent Biosensor for Hg2+ Detection through Hybridization Chain Reactions [DOI]
- 15 Base-Directed Formation of Fluorescent Silver Clusters [DOI]
- 16 Hairpins with Poly-C Loops Stabilize Four Types of Fluorescent Agn:DNA [DOI]
- 17 Ion-Tuned DNA/Ag Fluorescent Nanoclusters As Versatile Logic Device [DOI]
- 18 Signal-off Electrochemiluminescence Biosensor Based on Phi29 DNA Polymerase Mediated Strand Displacement Amplification for MicroRNA Detection [DOI]
- 19 DNA Templates for Fluorescent Silver Clusters and I-Motif Folding [DOI]
- 20 Occurrence, seasonal variation and risk assessment of antibiotics in the reservoirs in North China [DOI]
- 21 Label-free hairpin DNA-scaffolded silver nanoclusters for fluorescent detection of Hg2+ using exonuclease III-assisted target recycling amplification [DOI]
- 22 Simultaneous determination of eight β-lactam antibiotics in human serum by liquid chromatography–tandem mass spectrometry [DOI]
- 23 Total determination of chloramphenicol residues in foods by liquid chromatography-tandem mass spectrometry [DOI]
- 24 Use of antibiotics for adult upper respiratory infections in outpatient settings: a national ambulatory network study.
- 25 Metabolic profiling of intracellular metabolites in fermentation broths from β-lactam antibiotics production by liquid chromatography–tandem mass spectrometry methods [DOI]
- 26 A highly sensitive and selective aptamer-based colorimetric sensor for the rapid detection of PCB 77 [DOI]
- 27 A label-free and universal platform for antibiotics detection based on microchip electrophoresis using aptamer probes [DOI]
- 28 Biosensors for effective environmental and agrifood protection and commercialization: from research to market [DOI]
- 29 Microcalorimetrics Studies of the Thermodynamics and Binding Mechanism between l -Tyrosinamide and Aptamer [DOI]
- 30 Current bioanalytical methods for detection of penicillins [DOI]
- 31 Distinct Conformations of DNA-Stabilized Fluorescent Silver Nanoclusters Revealed by Electrophoretic Mobility and Diffusivity Measurements [DOI]
- 32 Amplified electrochemical detection of mecA gene in methicillin-resistant Staphylococcus aureus based on target recycling amplification and isothermal strand-displacement polymerization reaction [DOI]
- 33 Spectroscopic study of the interaction of insulin and its aptamer – sensitive optical detection of insulin [DOI]
- 34 Size-controlled Pd nanocluster grown by plasma gas-condensation method [DOI]
- 35 Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease [DOI]
- 36 Stem-directed growth of highly fluorescent silver nanoclusters for versatile logic devices [DOI]
- 37 Silver Nanomaterials Regulated by Structural Competition of G-/C-Rich Oligonucleotides [DOI]
- 38 Terminal deoxynucleotidyl transferase-induced DNAzyme nanowire sensor for colorimetric detection of lipopolysaccharides [DOI]
- 39 The light-up fluorescence of AgNCs in a “DNA bulb” [DOI]
- 40 Cloning and plant‐based production of antibody MC 10E7 for a lateral flow immunoassay to detect [4‐arginine]microcystin in freshwater [DOI]
- 41 Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output [DOI]
- 42 DNA Module Platform for Developing Colorimetric Aptamer Sensors [DOI]
- 43 Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards [DOI]
- 44 Preface for Special Topic: Few-atom metal nanoclusters and their biological applications [DOI]
- 45 Reducing Outpatient Antibiotic Prescribing for Acute Respiratory Infections: A Quasi-Experimental Study [DOI]
- 46 Sensitivity and Specificity of Various β-Lactam Antibiotics and Phenotypical Methods for Detection of TEM, SHV and CTX-M Extended-Spectrum β-Lactamases [DOI]
- 47 Naturally-Derived Antibiotics from Streptomyces zaomyceticus inhibit MRSA
- 48 A five year study on the susceptibility of isolates from various parts of the body
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