Formulation of Silver Nanoparticles using Gymnema sylvestre Leaf Extract and In-vitro Anti-diabetic Activity

Abstract

Aim: Formulation of Silver Nanoparticles Using Gymnema sylvestre Leaf Extract and In-Vitro Anti-diabetic Activity. Place and Duration of the Study: The present work has been carried out between April-2021 to July-2021 and at Mandasur University Mandsaur, Madhya Pradesh-458001. Methodology: A versatile technique was implemented for the synthesis of silver nanoparticles using aqueous and alcoholic extract of leaves of Gymnema sylvestre. The Ag-NP was subjected to UV, FTIR, XRD, and particle size analysis. The silver nanoparticles Gymnema sylvestre evaluated its anti-diabetic activity by inhibiting the enzymes α-amylase. Results: The reduction of silver nitrate to silver nanoparticles was confirmed by UV-Vis spectrophotometer showing a typical resonance (SPR) at about 400 nm which is specific for Ag-NPs. In FT-IR analysis the strong band peak at 3355 cm-1 in the extract is vibration bands, which may be due to the overlapping of amine N-H stretching bands. SEM and EDX images reveal that the particles are spherical and the relatively uniform shape of the silver nanoparticles was confirmed in the range of 70-100 nm in extract, whereas DLS showed good zeta potential and less particle size. Conclusions: The silver nanoparticles that were biologically synthesized from Gymnema sylvestre were economical, non-toxic, and environmentally benign. Due to the reducing and capping nature of the bioactive Phyto-compounds, present in the aqueous extracts. This extract is found to be suitable for the green synthesis of silver nanoparticles. Synthesized silver nanoparticles exhibit the highest level of anti-diabetic activity by inhibiting carbohydrate metabolizing enzymes such as α-amylase. Therefore, synthesized nanoparticles can be a good therapeutic agent for controlling diabetes by inhibiting enzymes that hydrolyze carbohydrates.