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Research Article Open access CC BY 3.0

HPTLC Methods for Determination of Gemifloxacin Mesylate in Rabbit Plasma

U. L. Narayan, B. Garnaik, S. K. Patro, S. Sahu

Journal of Pharmaceutical Research International · pp. 1707–1714 · Published 8 Jul 2014

10.9734/BJPR/2014/4070

Abstract

Aim: A new, simple, rapid, very sensitive and accurate high performance thin-layer chromatographic (HPTLC) method has been developed and validated for estimation of Gemifloxacin in rabbit plasma. Study Design: Validation study. Methodology: HPTLC was performed on silica gel 60F254 plates with ethanol: ethyl acetate: hexane, 2:7:1 (v/v), as mobile phase. Densitometry scanning was performed in absorbance mode at λ=254 nm. Result: The RF value was 0.21. The response was a linear function of concentration over the range 0.1–0.7μg mL−1 (r2=0.996). A maximum recovery of drug from plasma was obtained by using chloroform and glacial acetic acid. Mean extraction recovery was 80%. Intra-day and inter-day precision (% RSD) of the assay were in the range 1.19–2.85% and accuracy was 1.7-5.66% Conclusion: This method can be applied to pharmacokinetic studies in rabbit plasma.

HPTLC Gemifloxacin Rabbit plasma Liquid-liquid extraction.

References (18)

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