Skip to content
Research Article Open access CC BY 4.0

Human Sperm Cryopreservation- A Review

Balachandran Sivaraja Ganesan, G. Sumathy

Journal of Pharmaceutical Research International · pp. 460–466 · Published 29 Dec 2021

10.9734/jpri/2021/v33i63A36045

Abstract

Cryopreservation (CP) was first introduced sometime in the 1960s and since then process of CP of human sperm has been one of the effective procedures with respect to male fertility used in variety of cases prior to treatment for preserving donor and participant sperm cells prior to reproduction therapies like ART (Assisted Reproductive Therapy/Technology). CP of sperm is a common procedure for the people who are in condition like infertility due to ejaculation issues, infections, undescended testicles, hormonal imbalance etc., Several literatures are there on whether CP impacts sperm chromosomal integrity or the usage of a specific and unique freezing-thawing strategy. The purpose of this article is to discuss and bring to focus the effects of freezing and thawing on human spermatozoa and the changes in Acrosome integrity, mitochondria, DNA integrity, and plasma membrane integrity. In this age of technological advancements, after being used to treat infertility, CP acquired popularity in human medicine. It improves the effectiveness of assisted reproductive treatments by allowing all retrieved and/or fertilised cells to be saved for later use. Couples who conceive during their first treatment cycle can donate their unused frozen embryos to research under the CP programme. It enables people who are losing their fertility to maintain their reproductive cells and, in the future, conceive via assisted methods. It can be used by women who want to postpone childbirth or have a family history of early menopause. Similarly, the varied usage in critical applications makes CP the need of the hour.

Cryopreservation human sperm acrosome integrity mitochondria DNA & plasma membrane

References (32)

  1. 1 The causes of reduced fertility with cryopreserved semen [DOI]
  2. 2 Basic aspects of frozen storage of semen [DOI]
  3. 3 Oxidative Stress and Male Infertility: From Research Bench to Clinical Practice [DOI]
  4. 4 The effects of cryopreservation on sperm morphology, motility and mitochondrial function [DOI]
  5. 5 A lipid-phase separation model of low-temperature damage to biological membranes [DOI]
  6. 6 Implication of apoptosis in sperm cryoinjury [DOI]
  7. 7 Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity [DOI]
  8. 8 Cryopreservation of Sperm: Indications, Methods and Results [DOI]
  9. 9 DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification [DOI]
  10. 10 Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa [DOI]
  11. 11 Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa [DOI]
  12. 12 Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis [DOI]
  13. 13 Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen [DOI]
  14. 14 Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa [DOI]
  15. 15 Fertility Preservation [DOI]
  16. 16 The ultrastructural pathology of the spermatozoon as a cause of infertility: the role of electron microscopy in the evaluation of semen quality [DOI]
  17. 17 Relative contributions of the fraction of unfrozen water and of salt concentration to the survival of slowly frozen human erythrocytes [DOI]
  18. 18 Protective Effects of Ascorbate and Catalase on Human Spermatozoa During Cryopreservation [DOI]
  19. 19 Comparison of protective media and freezing techniques for cryopreservation of human semen [DOI]
  20. 20 Kinetic and Temporal Factors Influence Chilling Injury to Germinal Vesicle and Mature Bovine Oocytes [DOI]
  21. 21 A novel approach to sperm cryopreservation [DOI]
  22. 22 Advances in the slow freezing cryopreservation of microencapsulated cells [DOI]
  23. 23 Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa [DOI]
  24. 24 Effects of cryopreservation on human sperm acrosomes [DOI]
  25. 25 Cryopreservation of Human Semen [DOI]
  26. 26 Effect of Cooling, Freezing and Thawing Rates and Storage Conditions on Preservation of Human Spermatozoa [DOI]
  27. 27 Preservation techniques for human semen [DOI]
  28. 28 Semen cryobanking for men with cancer — Criteria change [DOI]
  29. 29 Effects of cryopreservation on plasma membrane glycoconjugates of human spermatozoa
  30. 30 Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test.
  31. 31 The Bioenergetics of Mammalian Sperm Motility [DOI]
  32. 32 Human Sperm Cryopreservation [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

3

Page views

0

PDF downloads

0

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.