Identification of Phytoconstituents using GC-MS and Determination of Antimicrobial and Antimycobacterial Activity of Boerhaavia diffusa L. Leaves

Abstract

Background: To identify bioactive phytoconstituents and evaluate antimicrobial and antimycobacterial potential of Boerhaavia diffusa leaves against selective human pathogens. Methods: The extract of Boerhaavia diffusa leaves was carried out by using methanol. Bioactive compounds was identified by GC-MS. Antimicrobial and antimycobacterial activity of methanolic extract Boerhaavia diffusa leaves was tested in vitro by Kirby-Bauer well diffusion method and rapid culture - MGITTM DST method against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus & Pseudomonas aeruginosa and Candida, Aspergillus and M. tuberculosis (H37RV ) and Mycobacterium tuberculosis bacteria resistant to Isoniazid & Rifampicine. Results: The obtained results of the GC-MS of Boerhavia diffusa led to the identity of 16 bioactive compounds. The crude extract showed antimicrobial activity against E.coli (Sensitive), Pseudomonas aeruginosa Sensitive and Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (Sensitive) but extract did not show antimicrobial effect against, E.coli (ESBL), Klebsiella pneumonia (Sensitive), Klebsiella pneumonia (ESBL), Pseudomonas aeruginosa (Resistant), Candida albicans and Aspergillus fumigates. The result of anti-mycobacterial activity showed that extract not showed antimycobacterial activity against M. tuberculosis (H37RV) and Mycobacterium tuberculosis bacteria resistant to Isoniazid & Rifampicine. Conclusion: The study result proved that methanolic extract of Boerhavia diffusa leaves has antimicrobial potential due to the presence of the bioactive compounds.