Skip to content
Research Article Open access CC BY 3.0

In vitro Studies of Iron Chelation Activity of Purified Active Ingredients Extracted from Triticum aestivum Linn. (Wheat Grass)

Priyabrata Das, Ashis Mukhopadhyay, Suvra Mandal, Bikas Chandra Pal, Raghwendra Mishra, Debarati Mukherjee, Soma Mukhopadhyay, Jayasri Basak, Manoj Kar

European Journal of Medicinal Plants · pp. 113–124 · Published 23 Feb 2012

10.9734/EJMP/2012/1059

Abstract

Objective: Seven to eight days germinated plants of Triticum aestivum (wheat grass) are a rich source of vitamin A, C, calcium, magnesium, phosphorus, potassium, sodium, sulphur, cobalt, zinc and protein. Traditionally the aqueous extract of T. aestivum was reported to be used as a health tonic in folk and ayurvedic medicine. We previously reported that aqueous extract of T. aestivum was found to reduce the blood transfusion requirement in iron overloaded Thalassemia and Myelodysplastic syndrome patients. Our objective was to extract and purify active ingredients from wheat grass and study their mode of action in stabilizing hemoglobin level in those patients. Design and Method: Active ingredients of wheat grass were extracted and purified by cation exchange column chromatography followed by High Performance Liquid Chromatography. In vitro experiments with phenylhydrazine treated red blood cell hemolysate were carried out before and after treatment of purified fraction of T. aestivum to study iron chelating activity. Result: Purified fraction of T. aestivum treated red blood cell showed significant inhibition of free reactive iron production and formation of thio-barbituric acid reactive substances when compared to desferrioxamine treated hemolysate. Conclusion: Iron chelating activity of purified fraction of T. aestivum, an inexpensive, easily available source, is very promising for further clinical trial and development of oral iron chelator drug for Thalassemia, Myelodysplastic syndrome and other iron overloaded diseases.

Iron chelation wheat grass extract mugineic acid thalassemia myelodysplastic syndrome HPLC iron

References (25)

  1. 1 Antioxidant activity applying an improved ABTS radical cation decolorization assay [DOI]
  2. 2 A new automated colorimetric method for measuring total oxidant status [DOI]
  3. 3 Iron-Chelating Therapy and the Treatment of Thalassemia [DOI]
  4. 4 Survival in Medically Treated Patients with Homozygous β-Thalassemia [DOI]
  5. 5 Results of Long-Term Iron-Chelating Therapy [DOI]
  6. 6 Elevated Carbon Dioxide Improves Plant Iron Nutrition through Enhancing the Iron-Deficiency-Induced Responses under Iron-Limited Conditions in Tomato [DOI]
  7. 7 [5] Deoxyribose assay for detecting hydroxyl radicals [DOI]
  8. 8 Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, divicine and isouramil [DOI]
  9. 9 Hydroxylated Phytosiderophore Species Possess an Enhanced Chelate Stability and Affinity for Iron(III) [DOI]
  10. 10 Wheat grass juice reduces transfusion requirement in patients with thalassemia major: a pilot study.
  11. 11 Lactoperoxidase-catalyzed lipid peroxidation of microsomal and artificial membranes [DOI]
  12. 12 Two Related Biosynthetic Pathways of Mugineic Acids in Gramineous Plants [DOI]
  13. 13 [32] Release of iron from hemoglobin [DOI]
  14. 14 Clinical experience of management of thalassemia: the Sardinian experience.
  15. 15 Effect of wheat grass tablets on the frequency of blood transfusions in Thalassemia Major [DOI]
  16. 16 Phenylhydrazine as a partial model for β‐thalassaemia red blood cell hemodynamic properties [DOI]
  17. 17 Chelation therapy in beta-thalassemia: the benefits and limitations of desferrioxamine.
  18. 18 Separation and determination of mugineic acid and its analogues by high-performance liquid chromatography [DOI]
  19. 19 Mugineic Acid Derivatives as Molecular Probes for the Mechanistic Elucidation of Iron Acquisition in Barley [DOI]
  20. 20 Electronic structure calculation study of metal complexes with a phytosiderophore mugineic acid [DOI]
  21. 21 The Treatment of Thalassemia -- Slow Progress and New Dilemmas [DOI]
  22. 22 Quantum chemical analysis of the deferiprone-iron binding reaction [DOI]
  23. 23 The Role of Iron Chelation Activity of Wheat Grass Juice in Blood Transfusion Requirement of Intermediate Thalassaemia. [DOI]
  24. 24 The role of iron chelation activity of wheat grass juice in patients with myelodysplastic syndrome [DOI]
  25. 25 Wheat grass juice in thalassemia.

Cited by 24

Green decontaminants for the remediation of chemical, biological, radiological and nuclear contaminants

Pooja Yadav, Raman Chawla, Varnika Khattar · Phytochemistry Reviews · 2025

Natural Iron Chelators as Potential Therapeutic Agents for the Treatment of Iron Overload Diseases

N. A. Sheikh, S. Kosalge, T. Desai · 2021

Wheat sprouts processing technology and powder receiving from the marcs with high content of the biological active substances

G. Gubanenko, E. Rechkina, L. V. Naymushina · Proceedings of the Voronezh State University of Engineering Technologies · 2019

PROTECTIVE ROLE OF POMEGRANATE JUICE BLENDED WITH WHEATGRASS AND LEMON EXTRACTS FOR RELIEVE CARBOHYDRATE INTOLERANCE

Hanaa G. Ishak, I. Ashoush, M. El-Hofi · Arab Universities Journal of Agricultural Sciences · 2019

Development of Wheatgrass‐Pomegranate Blended Therapeutical Juice Using Response Surface Methodology

H. Kashudhan, A. Dixit, Kshitiz Kumar · 2017

Optimization of ingredients for the development of wheatgrass based therapeutical juice using response surface methodology (RSM)

H. Kashudhan, A. Dixit, A. Upadhyay · 2017

The Comparison of the Chemical Composition, Sensory, Phenolic and Antioxidant Properties of Juices from Different Wheatgrass and Turfgrass Species

A. Özköse, D. Arslan, A. Acar · 2016

Chelators in Iron and Copper Toxicity

M. Lawson, M. Valko, M. Cronin · Current Pharmacology Reports · 2016

Mugineic acid, active ingredient of wheat grass: an oral novel hexadentate iron chelator in iron overloaded diseases.

P. Das, S. Mukhopadhyay, Nirmal Kumar Sarkar · Journal of Biochemistry (Tokyo) · 2016

Antioxidative and anticarcinogenic activities of methylpheophorbide a, isolated from wheat grass (Triticum aestivum Linn.)

P. Das, S. Mandal, S. Gangopadhyay · Natural Product Research · 2016

Showing 15 of 24 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

80

Page views

0

PDF downloads

16

Outbound clicks

24

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.