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Research Article Open access CC BY 4.0

In vitro Wound Healing Effect of a Siddha Formulation: Gandhaga Thailam

R. Sathish Adithya, E. M. Manikgantan, N. Kabilan, S. Kanimozhi

Asian Journal of Research in Medical and Pharmaceutical Sciences · pp. 77–85 · Published 4 Mar 2024

10.9734/ajrimps/2024/v13i1249

Abstract

Introduction: Wound is defined as disruption in the integrity of skin. Wound healing is a physiological process primarily initiated by vasoconstriction and platelet aggregation. Wound closure is completed by release of inflammatory markers and aggregation of fibroblasts which causes reepitheliazation. Gandhaga thailam (Medicated oil), a siddha formulation prepared from Gandhagam (sulphur), Vediuppu (Potassium nitrate), Manosilai (Arsenic disulphide), Navacharam (Ammonium Chloride), and Veeram (Mercuric per chloride) triturated with cow ghee is used in a variety of skin diseases. Scientific validation of the wound healing efficacy of Gandhaga thailam remains unveiled. Aim: The main aim of this research is to validate the wound healing efficacy of Gandhaga thailam through in vitro studies in L929 (Mouse fibroblast) cell line. Materials and Methods: L929 (Mouse fibroblast) cell line was cultured in a 25 cm 2 tissue culture flask with DMEM supplemented with 10% FBS, L-glutamine, sodium bicarbonate, and antibiotic solution containing: Penicillin (100 U/ml), Streptomycin (100 µg/ml), and Amphoteracin B (2.5 µg/ml). The scratch wounds were made by a sterile 1 mL pipette tip through a pre-marked line. The sample was checked for 0 hours, 24 hours, 48 hours, and 72 hours’. Results: The results of the study shows that Gandhaga thailam is efficient in wound healing and the wound is closed by about 48th hour after the scratch. The faster wound healing efficacy of Gandhaga thailam might be due to its potency to initiate Re- epithelialization. Conclusion: Thus it is evidenced that Gandhaga thailam has a potential wound healing activity.

Siddha cytotoxicity wound healing Gandhagam mineral based Scratch L929

References (21)

  1. 1 Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays [DOI]
  2. 2 Wound repair and regeneration [DOI]
  3. 3 Wound Healing--Aiming for Perfect Skin Regeneration [DOI]
  4. 4 Wound repair and regeneration: Mechanisms, signaling, and translation [DOI]
  5. 5 Challenges in the Treatment of Chronic Wounds [DOI]
  6. 6 The Molecular and Cellular Biology of Wound Repair [DOI]
  7. 7 Annual Review of Cell and Developmental Biology 2001 [DOI]
  8. 8 Cellular and molecular facets of keratinocyte reepithelization during wound healing [DOI]
  9. 9 Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts [DOI]
  10. 10 Mechanism Action of Platelets and Crucial Blood Coagulation Pathways in Hemostasis.
  11. 11 Challenges in Healing Wound: Role of Complementary and Alternative Medicine [DOI]
  12. 12 Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata [DOI]
  13. 13 Transcriptional Control of Wound Repair [DOI]
  14. 14 Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation [DOI]
  15. 15 An Investigation on the In Vitro Wound Healing Activity and Phytochemical Composition of Hypericum pseudolaeve N. Robson Growing in Turkey [DOI]
  16. 16 Nano-formulation of herbo-mineral alternative medicine from linga chenduram and evaluation of antiviral efficacy [DOI]
  17. 17 Purification, Detoxification, and Incineration Methods of Minerals and Metals in Traditional Medicine Formulations of Sri Lanka [DOI]
  18. 18 Epidermal Grafting for Chronic Complex Wounds in India: A Case Series [DOI]
  19. 19 Chemical analysis of Hg0-containing Hindu religious objects [DOI]
  20. 20 Standardization of Siddha Formulation “Gandhagapoora Parpam” by Using Modern Techniques [DOI]
  21. 21 Standardization of Gandhaga Thailam: A Traditional Siddha Formulation for Skin Disorders [DOI]

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