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Research Article Open access CC BY 4.0

Investigating the Modulatory Effect of Methanol Extract of Daniellia oliveri (ROLFE) Leaves on Mitochondrial Membrane Permeability Transition (MPT) Pore

Jonah Achem, Cosmos Ifeanyi Onyiba, Mobolaji T. Akinwole, Jemimah M. Malgwi, Omosola L. Bolarin, Olufunso O. Olorunsogo

International Journal of Biochemistry Research & Review · pp. 40–51 · Published 12 Nov 2020

10.9734/ijbcrr/2020/v29i930223

Abstract

Background: Mitochondrial-mediated cell death begins with opening of mitochondrial membrane permeability transition (mPT) pore and medicinal plants contain phytochemicals that modulate the mPT pore. Hypothesis and Purpose: We investigated the modulatory effects of crude methanol extract of Daniellia oliveri leaves (CMDO) on mPT pore in vitro. Study Design and Methods: Phytochemical screening and antioxidant activities of crude methanol extract of Daniellia oliveri leaves (CMDO) were evaluated according to standard procedures. CMDO was partitioned into chloroform fraction (CFDO), ethyl acetate fraction (EFDO) and methanol fraction (MFDO) by Vacuum liquid chromatography (VLC). Effects of CMDO, CFDO, EFDO and MFDO on mPT pore were assessed by spectrophotometry. Effects of the most potent fraction on mitochondrial ATPase, Fe-induced lipid peroxidation and cytochrome c release were assessed by spectrophotometry. CMDO was subjected to GC-MS analysis to identify the bioactive compounds present. Results: CMDO contains phytochemicals and showed appreciable total flavonoid content (0.483±0.02 QE mg/100g), total phenolic content (0.886±0.12 GAE mg/100g), total antioxidant capacity (0.039±0.001 AE mg/100 g), ferric antioxidant reducing power (IC50=350 µg/ml) and 2, 2-diphenyl-1 picrylhydrazyl (DPPH) radical scavenging activity (IC50=166 µg/ml). The maximum induction of mPT pore opening in the absence and presence of calcium, respectively, were as follows: CMDO (10.11 folds, 5.18 folds), CFDO (19.9 folds, 16.3 folds), EFDO (7.5 folds, 23.2 folds), MFDO (22.2 folds, 31.3 folds). The most potent mPT pore-opening fraction (MFDO) enhanced mitochondrial ATPase activity, inhibited Fe-induced lipid peroxidation and caused cytochrome c release. GC-MS analysis of CMDO revealed the presence of bioactive compounds including methyl propanamide, Dibutyl phthalate, saturated and unsaturated fatty acids. Conclusion: Methanol fraction (MFDO) of CMDO most potently induced mPT pore opening via enhancement of mitochondrial ATPase activity, which was substantiated by the release of cytochrome c (in vitro). This includes MFDO as a candidate pharmacologic remedy for diseases associated with insufficient apoptosis.

Daniellia oliveri Mitochondria mPT pore F0F1 ATPase Cytochrome c apoptosis.

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Cited by 4

Experimental and computational insights into mitochondrial permeability transition and pro-apoptotic signaling induced by the methanol fraction of Daniellia oliveri leaves in male Wistar rats

J. Achem, Oludare Michael Ogunyemi, O. Banwo · Egyptian Journal of Basic and Applied Sciences · 2025

Gliclazide and Crassocephalum rubens leaf extract inhibit glucose-induced mitochondrial permeability transition pore (MPTP) opening in isolated pancreas mitochondria of Wistar rats

T. Obafemi, B. Afolabi, J. Falode · Journal of HerbMed Pharmacology · 2022

Methanol Fraction of Daniellia oliveri (ROLFE) Leaves Induces Mitochondrial-mediated Apoptosis in Rats via Alteration of Liver Mitochondrial Viability

J. Achem, Cosmos Ifeanyi Onyiba, O. Banwo · 2023

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