Isolation and Characterization of Bacteriophage against Drug-resistant Staphylococcus aureus
Sajib Kumar Saha, Md. Ashiqur Rahman, Md. Sultan Mahmud, Md. Taijul Islam, Md. Nazmul Islam, Sadia Islam, Shohanur Rahaman, Anika Zafreen, Md. Raihanul Islam, Md. Sujon Ali
Journal of Advances in Microbiology · pp. 128–138 · Published 12 Oct 2023
10.9734/jamb/2023/v23i10763Abstract
Staphylococcus aureus is one of the most successful pathogens due to its vast arsenal of virulence factors, including anchor proteins, secreted toxins and enzymes, polysaccharides, and immune system modulators. Bacteriophages are viruses that specifically target and infect bacterial cells. Bacteriophage is studied widely to improve the safety of foods and prevent food borne diseases of bacterial etiology, as well as to reduce the use of antibiotics in livestock. Besides, therapeutic applications of bacteriophage in human and the usage of phages in veterinary medicine and agriculture are also highly assessed. Hence, the present study aimed to isolate and to characterize Staphylococcus aureus specific bacteriophage. Bacteriophages were isolated from various environmental water samples. After incubation growth occurred in MSA and NA plate. On Staphylococcus aureus, antibiotic discs showed some useful identifying zones. It was performed with the use of five antibiotics: Penicillin, Oxacillin, Tetracyclin, Gentamycin and Cefoxitin. Penicillin, Oxacillin are showing no zones, for that reason showed resistant pattern. Environmental phages were then selected based on the size and clarity of their plaques. Bacteriophage produce some clear zone against bacteria. The isolated bacteriophages were found to be able to lyse the Staphylococcus aureus. S. aureus was isolated on nutrient agar media and mannitol salt agar media and confirmed by biochemical test like catalase test, coagulase test and oxidase test. Drug-resistant S. aureus also form some specific zones in the nutrient agar plates. Then allowed to compare this two specific zones stability.
References (6)
- 1 Phage therapy: past history and future prospects.
- 2 The strange history of phage therapy [DOI]
- 3 Re-establishing a place for Phage Therapy in Western Medicine [DOI]
- 4 Isolation and Preliminary Characterization of Lytic and Lysogenic Phages with Wide Host Range within the Streptomycetes [DOI]
- 5 Size does matter – distinguishing bacteriophages by genome length (and ‘breadth’) [DOI]
- 6 Editorial Commentary: Phage Therapy: Quo Vadis? [DOI]
Cited by 10
Md. Sultan Mahmud, Md. Ashiqur Rahman, Md. Nazmul Islam, Sadia Islam, Sajib Kumar Saha, Md. Taijul Islam, Anny Khatun, Silva Adhikari · Asian Journal of Research in Medical and Pharmaceutical Sciences · 2023
Sima Sikdar, Krishnendu Manna, Aparna Shil · The Microbe · 2026
Sidra Munir, Syed Abdullah Mazhar, Nazish Mazhar Ali · 2024
Showing 3 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Prevalence of Schistosomia haematobium and Staphylococcus aureus among Pregnant Women in Akure — shares topic coverage
- Carriage of Drug-Resistant Staphylococcus aureus in the Anterior Nares of a Healthy Student Population — shares topic coverage
- Two Endogenous Endophthalmitis Cases Accompanying Nephrological Condition in Diabetic Patients — shares topic coverage
- Antibiotic Susceptibility Patterns in Adenotonsillar Bacterial Isolates in a Tertiary Hospital in Sub-Saharan Africa — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
57
Page views
0
PDF downloads
16
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.