Leaf Extracts of Vernonia amygdalina Del. from Northern Ghana Contain Bioactive Agents that Inhibit the Growth of Some Beta-lactamase Producing Bacteria in vitro
Oseni Lateef Adebayo, Abugri James, Sumabe Balagra Kasim, Onilimor Peter Jagri
Journal of Pharmaceutical Research International · pp. 192–202 · Published 24 Oct 2013
10.9734/BJPR/2014/3200Abstract
Aims: The current study was aimed at evaluating the phytochemical profile and in vitro bacteria growth inhibitory potential of different solvent leaf extracts of V. amygdalina from northern Ghana. Study Design: Different solvent extracts of the plant were quantitatively and qualitatively evaluated for phytochemicals. In vitro bacteria sensitivity assay of the extracts was evaluated using some beta-lactamase producing bacteria as test microbes. Methodology: Ethanolic, methanolic, petroleum ether and aqueous leaf extracts of Vernonia amygdalina were studied in vitro for growth inhibition against beta-lactamase producing bacteria such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa using agar well diffusion method. Saponins, flavonoids, glycosides, alkaloids, tannins, phenolics, reducing sugars, anthracenosides, terpenes and phytosteroids were determined qualitatively and quantified. Results: All the phytochemicals tested were found to be present in both the methanolic and ethanolic leaf extracts. The aqueous extract showed the presence of saponins, reducing sugars and anthracenosides. Glycosides, flavonoids, saponins and alkaloids were the only groups of phytochemicals found in the petroleum ether extract. The methanolic extract showed the greatest amount of saponins (14.23%), flavonoids (2.15%), alkaloids (7.49%), tannins (5.4%), terpenes (10.20%) and phenolics (8.24%). The methanolic extract at concentration of 4 mg/ml showed growth inhibitory activities against all the test organisms with zone of inhibitions ranging from 16.00+0.50 (against E. coli) to 20.50+0.03 mm (against S. aureus). The ethanolic extract showed activity against only two of the test organisms viz. 23.00+0.33 mm against P. aeruginosa and 12.00+0.00 mm against S. aureus at similar concentration. All test organisms were resistant to both aqueous and pet ether extracts. Conclusion: The antibacterial activities of the methanolic and ethanolic extracts were significant (P < 0.05) and may be mediated by the presence of saponins, flavonoids, tannins, terpenes and alkaloids. Results from present study corroborate previous findings and also presents methanolic leaf extract of the plant as a credible candidate for the discovery of new phytotherapeutic agents against the beta-lactamase producing bacteria tested.
References (25)
- 1 Medicinal plants and traditional medicine in Africa
- 2 Phytochemical constituents of some Nigerian medicinal plants [DOI]
- 3 Trease and Evans' Pharmacognosy
- 4 Natural Product Drug Discovery and Development: New Perspectives on International Collaboration [DOI]
- 5 Observations on the illness and consumption of a possibly medicinal plantVernonia amygdalina (Del.), by a wild chimpanzee in the Mahale Mountains National Park, Tanzania [DOI]
- 6 Current Status of the NCI Plant and Animal Product Program [DOI]
- 7 Bioactive sesquiterpene lactones from the leaves of Vernonia amygdalina [DOI]
- 8 An ethnobotanical study of plants used for the treatment of sexually transmitted diseases (njovhera) in Guruve District, Zimbabwe [DOI]
- 9 Genetic Background ofEscherichia coliand Extended-spectrum β-Lactamase Type [DOI]
- 10 In vivo antimalarial activity of Vernonia amygdalina [DOI]
- 11 Antimicrobial-resistant InvasiveEscherichia coli, Spain [DOI]
- 12 Changing strategies in natural products chemistry [DOI]
- 13 β‐Lactam resistance in Streptococcus pneumoniae: penicillin‐binding proteins and non‐penicillin‐binding proteins [DOI]
- 14 Theory and Application of Microbiological Assay [DOI]
- 15 Anti-inflammatory and antimicrobial activities of triterpenoids from Strobilanthes callosus Nees [DOI]
- 16 A Novel Natural Inhibitor of Extracellular Signal-Regulated Kinases and Human Breast Cancer Cell Growth [DOI]
- 17 Nutritional Assessment of Vernonia amygdalina Leaves in Growing Mice [DOI]
- 18 Antimicrobial activity of Vernonia amygdalina leaves [DOI]
- 19 Vemoniosides D and E, Two Novel Saponins from Vernonia amygdalina [DOI]
- 20 Antibacterial activity and phytochemical analysis of leaf extracts of Lasienthera africanum [DOI]
- 21 Bitter principle and a related steroid glucoside from Vernonia amygdalina, a possible medicinal plant for wild chimpanzees. [DOI]
- 22 Antibacterial properties of Uvaria chamae, Congronema latifolium, Garcinia kola, Vemonia amygdalina and Aframomium melegueta [DOI]
- 23 Evaluation Of Bioactivity Of Various Indian Medicinal Plants – An In-Vitro Study [DOI]
- 24 ASSAY DESIGN AND EVALUATION [DOI]
- 25 A comparative assessment on regeneration status of indigenous woody plants in Eucalyptus grandis plantation and adjacent natural forest
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