Loop-Mediated Isothermal Amplification (LAMP): Comparative Advances over Conventional PCR and Other Molecular Techniques
Yahaya Hassan, Leslie Thian Lung Than
Annual Research & Review in Biology · pp. 33–44 · Published 8 Aug 2020
10.9734/arrb/2020/v35i830257Abstract
Gene amplification technology is essential in the fields of diagnostic medicine. The polymerase chain reaction (PCR) is central in the molecular studies and provides ways for diagnostic advancement in the areas. However, the requirement for thermal cycler in a dedicated facility for amplification of target genes in the PCR technique has been a bottleneck to many researchers. The limitations associated with PCR include cost implication, strict expertise necessity and relatively higher turn-around time. The emergence of loop-mediated isothermal amplification (LAMP) in the last two decades assists in bridging the undesirable gaps. This review aims to highlight the natural advantages of the LAMP technique over the existing conventional PCR and other isothermal molecular techniques. Available published articles on LAMP techniques reviewed, listed many outstanding advances of the method in comparison to traditional PCR technique. The mentioned advantages include simplicity, affordability, naked-eye result detection and many more. That made LAMP becomes a rapidly accepted method in the field of molecular diagnosis. Other essential features of LAMP in comparison with other emerging nucleic acid amplification techniques were adequately explained and presented in tabular form for research and quick reference purposes. Though LAMP has some few limitations, its advantages outweigh its flaws by filling the gap in the field of molecular biology diagnostics.
References (49)
- 1 Loop-mediated isothermal amplification of DNA [DOI]
- 2 Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects [DOI]
- 3 A guide to aid the selection of diagnostic tests [DOI]
- 4 Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP) [DOI]
- 5 “Paper Machine” for Molecular Diagnostics [DOI]
- 6 Loop-mediated isothermal amplification (LAMP): recent progress in research and development [DOI]
- 7 Loop-mediated isothermal amplification (LAMP): A novel rapid detection platform for pathogens [DOI]
- 8 An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics [DOI]
- 9 Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification [DOI]
- 10 The Point-of-Care Laboratory in Clinical Microbiology [DOI]
- 11 Diagnostic Devices for Isothermal Nucleic Acid Amplification [DOI]
- 12 Gold Nanoparticles for Diagnostics: Advances towards Points of Care [DOI]
- 13 The application of loop-mediated isothermal amplification (LAMP) in food testing for bacterial pathogens and fungal contaminants [DOI]
- 14 GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use [DOI]
- 15 Recent trends in molecular diagnostics of yeast infections: from PCR to NGS [DOI]
- 16 Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases [DOI]
- 17 Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection [DOI]
- 18 Loop-Mediated Isothermal Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Enterococcus faecalis and Staphylococcus aureus [DOI]
- 19 Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates [DOI]
- 20 A Strong Strand Displacement Activity of Thermostable DNA Polymerase Markedly Improves the Results of DNA Amplification [DOI]
- 21 Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes [DOI]
- 22 Loop‐mediated isothermal amplification for detection of the tomato and potato late blight pathogen, P hytophthora infestans [DOI]
- 23 Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids [DOI]
- 24 Current state and future perspectives of loop-mediated isothermal amplification (LAMP)-based diagnosis of filamentous fungi and yeasts [DOI]
- 25 The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Vibrio parahaemolyticus [DOI]
- 26 Recurrent candidal intertrigo: challenges and solutions [DOI]
- 27 A self-contained microfluidic in-gel loop-mediated isothermal amplification for multiplexed pathogen detection [DOI]
- 28 Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis [DOI]
- 29 Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods [DOI]
- 30 Derivatives of Bst-like Gss-polymerase with improved processivity and inhibitor tolerance [DOI]
- 31 Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification [DOI]
- 32 The effects of metal ion PCR inhibitors on results obtained with the Quantifiler® Human DNA Quantification Kit [DOI]
- 33 Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms [DOI]
- 34 Comparison of a loop-mediated isothermal amplification for orf virus withquantitative real-time PCR [DOI]
- 35 Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout [DOI]
- 36 Development of multiplex loop-mediated isothermal amplification assays to detect medically important yeasts in dairy products [DOI]
- 37 Rapid visual identification of bovine meat by loop mediated isothermal amplification combined with immunochromatographic strip [DOI]
- 38 PMA-LAMP for rapid detection of Escherichia coli and shiga toxins from viable but non-culturable state [DOI]
- 39 Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye [DOI]
- 40 Advances in multiplex nucleic acid diagnostics for blood-borne pathogens: promises and pitfalls [DOI]
- 41 Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species [DOI]
- 42 Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran [DOI]
- 43 Loop Mediated Isothermal Amplification (LAMP) Test- a Novel Nucleic Acid Based Assay for Disease Diagnosis [DOI]
- 44 Evaluation of a rapid detection for Coxsackievirus B3 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) [DOI]
- 45 Self-sustained sequence replication (3SR): an alternative to PCR [DOI]
- 46 Use of reverse transcriptase loop-mediated isothermal amplification assay for field detection of Newcastle disease virus using less invasive samples [DOI]
- 47 LOOP mediated isothermal AMPlification (LAMP) in diagnosis of neorocryptococcosis [DOI]
- 48 Applied Microbiology and Biotechnology [DOI]
- 49 The journal of veterinary medical science
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