Skip to content
Research Article Open access CC BY 4.0

MicroRNAs miR-1 and miR-206 Regulate Monocarboxylate Transporter-4 and Vascular Endothelial Growth Factor Gene Expression in Colorectal Cancer

Anas Khaleel, Rowan AlEjielat, Cristina I. Batarseh, Walid Abu Rayyan, Abdallah Ahmed Elbakkoush, Amneh Tarkhan, Aiman Mahdi, Yazan S. Batarseh

Journal of Pharmaceutical Research International · pp. 141–152 · Published 6 Aug 2020

10.9734/jpri/2020/v32i1330594

Abstract

Background: Colorectal cancer (CRC) is currently the third most common cancer type in males and the second most occurring in females. The role of microRNA (miRNA) in the development of colorectal cancer is not fully elucidated. Therefore, understanding the mechanistic interaction between miRNA and their target oncogenes may hold great importance as a possible target for interventional anticancer therapy. Aims: To identify miRNAs that are part of the regulating pathway of Monocarboxylate Transporter-4 (MCT4) and Vascular Endothelial Growth Factor (VEGF) oncogenes. Study Design: We used publicly available prediction tools (e.g. TargetScan, MicroCosm, PicTar, and DIANA-microT-CDS) to identify the possible miRNA that target the two oncogenes. Methodology: We used the GeneMania database to visualize the network and verify gene names and remove ambiguity and duplications.  Furthermore, we used miRTarBase database to identify experimentally validated targets which we used to further confirm miRNA-oncogene relationships.  Finally, we utilized miR-Mfold web-tool to further visualize the circular structures and the simulated miR-1 and miR-206 targeting arrangements. Results: We found two putative miRNA (miR-1 and miR-206) that may downregulate MCT4 coded by SLC16A3 gene and VEGF which is coded by VEGF gene. We found relationships between the validated target genes of miR-1 and miR-206 through GeneMania which we extracted from the literature. And we elucidated the proposed structure of these two miRNAs through miR-Mfold web-tool. Conclusion: Our results elucidated a novel regulation pathway in CRC cells and may suggest a potential therapeutic approach for CRC therapy. MiR-1 and miR-206 may help cells go to apoptosis and inhibit the angiogenesis of colorectal cancer cells by down-regulation of MCT4 and VEGF proteins in tumor tissues.

MCT4 colorectal cancer VEGF microRNA miR-1 miR-206.

References (39)

  1. 1 Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles [DOI]
  2. 2 Mfold web server for nucleic acid folding and hybridization prediction [DOI]
  3. 3 Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets [DOI]
  4. 4 The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function [DOI]
  5. 5 miRBase: microRNA sequences, targets and gene nomenclature [DOI]
  6. 6 Combinatorial microRNA target predictions [DOI]
  7. 7 DIANA-microT web server v5.0: service integration into miRNA functional analysis workflows [DOI]
  8. 8 Cancer genetics and genomics of human FOX family genes [DOI]
  9. 9 Down-regulation of Micro-RNA-1 (miR-1) in Lung Cancer: Suppression of Tumorigenic Property of Lung Cancer Cells and Their Sensitization to Doxorubicin-Induced Apoptosis by miR-1 [DOI]
  10. 10 Transcription factor NRF2 regulates miR-1 and miR-206 to drive tumorigenesis [DOI]
  11. 11 Monocarboxylate Transporter 4 Regulates Maturation and Trafficking of CD147 to the Plasma Membrane in the Metastatic Breast Cancer Cell Line MDA-MB-231 [DOI]
  12. 12 MiR-145 directly targets p70S6K1 in cancer cells to inhibit tumor growth and angiogenesis [DOI]
  13. 13 The tumour-suppressive function of miR-1 and miR-133a targeting TAGLN2 in bladder cancer [DOI]
  14. 14 MicroRNA-1/206 Targets c-Met and Inhibits Rhabdomyosarcoma Development [DOI]
  15. 15 MicroRNA Signature Distinguishes the Degree of Aggressiveness of Papillary Thyroid Carcinoma [DOI]
  16. 16 Extracellular Vesicles Modulate the Glioblastoma Microenvironment via a Tumor Suppression Signaling Network Directed by miR-1 [DOI]
  17. 17 MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation [DOI]
  18. 18 Tumour suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer [DOI]
  19. 19 Monocarboxylate transporter 4 (MCT4) and CD147 overexpression is associated with poor prognosis in prostate cancer [DOI]
  20. 20 miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma [DOI]
  21. 21 MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer [DOI]
  22. 22 MicroRNA-22 Regulates Hypoxia Signaling in Colon Cancer Cells [DOI]
  23. 23 miRNA Profiling in Colorectal Cancer Highlights miR-1 Involvement in MET-Dependent Proliferation [DOI]
  24. 24 MiR-1 suppresses tumor cell proliferation in colorectal cancer by inhibition of Smad3-mediated tumor glycolysis [DOI]
  25. 25 miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control [DOI]
  26. 26 Functional role of LASP1 in cell viability and its regulation by microRNAs in bladder cancer [DOI]
  27. 27 Identification of novel molecular targets regulated by tumor suppressive miR-1/miR-133a in maxillary sinus squamous cell carcinoma [DOI]
  28. 28 Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer [DOI]
  29. 29 MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells [DOI]
  30. 30 VEGF-1 expression in colorectal cancer is associated with disease localization, stage, and long-term disease-specific survival.
  31. 31 MicroRNA-93 inhibits tumor growth and early relapse of human colorectal cancer by affecting genes involved in the cell cycle [DOI]
  32. 32 Tumor suppressive microRNA-1 mediated novel apoptosis pathways through direct inhibition of splicing factor serine/arginine-rich 9 (SRSF9/SRp30c) in bladder cancer [DOI]
  33. 33 miRNA-1 targets fibronectin1 and suppresses the migration and invasion of the HEp2 laryngeal squamous carcinoma cell line [DOI]
  34. 34 MicroRNA-1 and microRNA-499 downregulate the expression of the ets1 proto-oncogene in HepG2 cells [DOI]
  35. 35 Failure to downregulate the BAF53a subunit of the SWI/SNF chromatin remodeling complex contributes to the differentiation block in rhabdomyosarcoma [DOI]
  36. 36 Expression profiles of microRNAs and their target genes in papillary thyroid carcinoma [DOI]
  37. 37 MiR-1 targets PIK3CA and inhibits tumorigenic properties of A549 cells [DOI]
  38. 38 MicroRNA-1 targets Slug and endows lung cancer A549 cells with epithelial and anti-tumorigenic properties [DOI]
  39. 39 Expression of vascular endothelial growth factor and its receptor KDR (kinase domain-containing receptor)/Flk-1 (fetal liver kinase-1) as prognostic factors in human colorectal cancer [DOI]

Cited by 0

No indexed citations yet.

Article metrics

Real usage data collected on this platform.

18

Page views

0

PDF downloads

1

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.