Skip to content
Research Article Open access CC BY 4.0

Molecular Characterisation and Identification of Three Mushrooms Found in the Niger Delta Region

S. B. Chuku, E. O. Nwachukwu, I. O. Agbagwa, H. O. Stanley

Annual Research & Review in Biology · pp. 115–121 · Published 4 Aug 2020

10.9734/arrb/2020/v35i730253

Abstract

Mushrooms are a group of fungi that are diverse and in the Niger Delta region; there are various species, some of which share similar morphological features. Over the years, mushrooms have been put to several important uses ranging from food, nutraceuticals, feed for livestock, and more recently bioremediation, hence, the need for an accurate approach of identification is eminent. This study aims to identify three mushroom samples with the potential for crude oil degradation using molecular tools. The molecular identification of the mushrooms was carried out with the ITS (Internal Transcribed Spacer) region to analyze their genetic diversity. The three mushroom samples were identified as Pleurotus ostreatus, Pleurotus floridanus and Lentinus squarrosulus. The blast results showed 86.0%, 97.9%, and 88.4% for Pleurotus ostreatus, Pleurotus floridanus and Lentinus squarrosulus respectively for sequence similarity. The samples were assigned unique accession numbers on GenBank The use of molecular characterization gives reliable results to the species level. The results from this study have increased the database of Pleurotus and Lentinus DNA and serve as a basis for the identification of unidentified species by comparing their PCR amplified sequences with ITS primers.

Molecular characterization Internal Transcribed Spacer (ITS) mushroom

References (19)

  1. 1 MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [DOI]
  2. 2 AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS [DOI]
  3. 3 ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases [DOI]
  4. 4 Feasibility of bioremediation by white-rot fungi [DOI]
  5. 5 Agarose Gel Electrophoresis for the Separation of DNA Fragments [DOI]
  6. 6 Microorganisms relevant to bioremediation [DOI]
  7. 7 DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis [DOI]
  8. 8 Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains [DOI]
  9. 9 The white-rot fungusPleurotus ostreatussecretes laccase isozymes with different substrate specificities [DOI]
  10. 10 Uses of mushrooms in bioremediation: A review [DOI]
  11. 11 An investigation of fungal contamination on the surface of medicinal herbs in China [DOI]
  12. 12 Sequence variation of Pleurotus species collected from Eastern Asia
  13. 13 Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium [DOI]
  14. 14 Improvement of Laccase Production in Pluerotus pulmonarius-LAU 09 by Mutation [DOI]
  15. 15 Mushroom: molecular characterization of indigenous species in the Niger Delta Region of Nigeria [DOI]
  16. 16 Studies on biodiversity of some mushrooms collected in Lagos State, Nigeria using biotechnological methods [DOI]
  17. 17 Submitting a Sequence to GenBank [DOI]
  18. 18 Comparative study on DNA extraction methods for PCR amplification of COI gene from ascidians of Indian coast
  19. 19 Effects of selected culture media on mycellial growth of oyster mushroom (Pleurotus ostreatus) [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

49

Page views

0

PDF downloads

11

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.