Mycological Medium for the Production of Antibiotics against Urogenital Bacterial Pathogens from Natural Sources

Abstract

Aim: This study was carried out to evaluate a mycological medium for the production of antibiotics from natural sources. Methodology: Garden soil samples were randomly collected and screened for the growth of Aspergillus species. The isolated fungi were grown in a formulated mycological medium that comprises Phoenix dactylifera (PD) fruits, Chrysophyllum albidum (CA) fruits, Glycine max (GM) peel and Musa paradisiaca (MP) peel, for the production of antibiotics, considering varying conditions (pH, temperature, carbon sources and nitrogen sources).The antibiotic were exposed to urogenital pathogens using agar welled diffusion method. The antibiotic was precipitated, eluted using chromatographic techniques and structurally elucidated using GS-MS technique. Data obtained were appropriately analyzed. Results: The fungal species isolated were Aspergillus flavus strain HUS6 (AFH6), Aspergillus niger strain HG48 (ANH48) and Aspergillus tubingiensis strain EM-CN1 (ATE1). The optical pH and temperature for the growth and production of potent antibiotics by the fungal isolates were 7.0 and 25. Sugar extracted from Phoenix dactylifera fruits and NOdZ (a prepared mixture of Rhizobium leguminosarum, soybean meal and Arachis hypogoea nodule meal) respectively showed the highest significant (P<0.05) carbon and nitrogen content in the production medium. The eluates; oleic acid (H1) > 1-docosene (H2) > 1-octadecene (H3) > 9-octadecenoic acid (Z) methyl ester (H5) from ANH48 showed significant (P<0.05) pronounced activities against bacteria implicated in urogenital tract infections; Escherichia coli HH35 (ECH3), Klebsiella pneumoniae GH44 (KPG4) and Proteus mirabilis K1609 (PMK1). Similar results were recorded from the eluates; oleic acid (X3) > 9-octadecenoic acid (Z) methyl ester (X1) > cyclohexanone-3-hydroxyl (X2) from AFH6, and hexacosanoic acid (T2) from ATE1 against the Urogenital bacterial isolates. Conclusion: From the study, the fractions extracted from AFH6, ANH48 and ATE1 were potent antibiotics that showed pronounced activities against urogenital bacterial pathogens.