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Research Article Open access CC BY 3.0

New Experimental Model of Brain Tumors in Brains of Adult Immunocompetent Rats

Vladimir P. Baklaushev, Vadym M. Kavsan, Olena V. Balynska, Gaukhar M. Yusubalieva, Maxim A. Abakumov, Vladimir P. Chekhonin

Journal of Advances in Medicine and Medical Research · pp. 206–215 · Published 9 Mar 2012

10.9734/BJMMR/2012/1072

Abstract

Aims: Xenograft models, namely heterotransplantation of human cancer cells or tumor biopsies into immunodeficient rodents are the major preclinical approach for the development of novel cancer therapeutics. However, in these models the animals must be used only after the severe systemic immune suppression in order to ensure graft survival. Thus, additional new human brain tumor models without immune suppression of the recipient rodent may be required. Place and Duration of Study: Laboratory of Immunochemistry, V.P. Serbsky National Research Centre for Social and Forensic Psychiatry and Department of Nanobiotechnology, N.I. Pirogov Russian State Medical University and Department of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics between June 2009 and July 2010. Methodology: Brain tumor modeling was performed by intracerebral stereotactic implantation of cells to the healthy adult rats without any artificial immunodepression. Cells were implanted to the striatum region of ketamine-anesthetized rats at specific coordinates according to Swanson's rat brain atlas. Tumor growth was monitored weekly via registration of neurological signs and in vivo Bruker MRI system. Results: On the 21st day after implantation of C6 glioma, U251 or 293_CHI3L1 cells severe neurological deficit appeared in rats. Huge intracerebral tumors were found in each animal under investigation while no tumor growth was observed for at least 8 weeks in rats injected with empty vector-transfected 293 cells. Tumors contained the dense superficial cell layer and prominent lobules with central newly ingrowing blood vessels. Histological assay revealed displacement of median cerebral structures and hydrocephalus in contralateral hemisphere. All tumors were surrounded by numerous GFAP-positive reactive astrocytes. Conclusion: Positive results with transplantation of 293_CHI3L1 cells into adult rat brains without any immunosupression show the validity of this animal model. In all experiments such implantations provoked malignant tumor formation while there were no visible tumors in control rats. We believe this to be the first animal model of human brain tumor that displays the possibility to study various biologic features of and host therapeutic response to brain tumor in an immunocompetent host.

Brain tumors immunocompetent heterotransplantation xenograft models.

References (27)

  1. 1 Differentiated Rat Glial Cell Strain in Tissue Culture [DOI]
  2. 2 Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells [DOI]
  3. 3 Rat brain tumor models in experimental neuro-oncology:The 9L, C6, T9, F98, RG2 (D74), RT-2 and CNS-1 Gliomas [DOI]
  4. 4 Site-Specific Migration and Neuronal Differentiation of Human Neural Progenitor Cells after Transplantation in the Adult Rat Brain [DOI]
  5. 5 Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats [DOI]
  6. 6 Transplantation of Human Neural Progenitor Cells into the Neonatal Rat Brain: Extensive Migration and Differentiation with Long-Distance Axonal Projections [DOI]
  7. 7 Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43 [DOI]
  8. 8 Modeling and immunohistochemical analysis of C6 glioma In Vivo [DOI]
  9. 9 Astrocyte cell lineage. V. Similarity of astrocytes that form in the presence of dBcAMP in cultures to reactive astrocytes in vivo [DOI]
  10. 10 Leukocyte infiltration and glial reactions in xenografts of mouse brain tissue undergoing rejection in the adult rat brain. A light and electron microscopical immunocytochemical study [DOI]
  11. 11 CNS immunological modulation of neural graft rejection and survival [DOI]
  12. 12 Experimental model and immunohistochemical analyses of U87 human glioblastoma cell xenografts in immunosuppressed rat brains.
  13. 13 Visualization of Connexin 43-positive cells of glioma and the periglioma zone by means of intravenously injected monoclonal antibodies [DOI]
  14. 14 Targeted Transport of 125I-Labeled Antibody to GFAP and AMVB1 in an Experimental Rat Model of C6 Glioma [DOI]
  15. 15 Identification of human cells in brain xenografts and in neural co-cultures of rat by in situ hybridisation with Alu probe [DOI]
  16. 16 Gene Encoding Chitinase 3-Like 1 Protein (CHI3L1) is a Putative Oncogene [DOI]
  17. 17 The immune response to intracerebral neural grafts [DOI]
  18. 18 Behavior of human neural progenitor cells transplanted to rat brain [DOI]
  19. 19 Development of Neural Stem/Progenitor Cells from Human Brain by Transplantation into the Brains of Adult Rats [DOI]
  20. 20 Characterization of new cell line stably expressing CHI3L1 oncogene [DOI]
  21. 21 Study of the Efficiency of Transplantation of Human Neural Stem Cells to Rats with Spinal Trauma: the Use of Functional Load Tests and BBB Test [DOI]
  22. 22 Immunological reaction and blood-brain barrier in mouse-to-rat cross-species neural graft [DOI]
  23. 23 Suppression of immunorejection against rat fetal dopaminergic neurons in a mouse brain by 15-deoxyspergualin [DOI]
  24. 24 A Serotonin‐Hippocampal Model Indicates Adult Neurons Survive Transplantation and Aged Target May be Deficient in a Soluble Serotonergic Growth Factora [DOI]
  25. 25 Survival of Astroglial Cell Lineage from Adult Brain Transplant [DOI]
  26. 26 Short‐term post‐grafting morphological alterations of glia from an adult brain transplant [DOI]
  27. 27 [Influence of the neural stem cell transplantation on the restoration of CNS functions in rats with cortical stroke].

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