Optimization of the Cultural Parameters for Improved Production of Antimicrobial Metabolites by Streptomyces gulbargensis DAS 131
Usha Kiranmayi Mangamuri, Muvva Vijayalakshmi, Sudhakar Poda, Dayanand Agasar
Journal of Pharmaceutical Research International · pp. 1130–1145 · Published 26 Apr 2014
10.9734/BJPR/2014/7085Abstract
Aims: To investigate the influence of appropriate culture medium by optimizing the cultural conditions affecting the growth and bioactive metabolite production by Streptomyces gulbargensis DAS 131 under submerged culture conditions in order to reduce the cost of fermentation process to improve the formation of antimicrobial compounds. Place and Duration of Study: Department of Botany and Microbiology, January 2012 to May 2012. Methodology: The impact of environmental parameters such as incubation period, pH, temperature and salt concentration and effect of various nutrients such as carbon and nitrogen sources and minerals on the antimicrobial metabolite production by Streptomyces gulbargensis DAS 131 was evaluated by employing agar well diffusion assay. Growth was measured in the form of dry mycelial weight. Results: The optimum pH and temperature for bioactive metabolite production were 7 and 35°C respectively. Highest antimicrobial metabolite production was found when the strain was inoculated into the medium amended with glucose at the concentration of 2%, soya peptone at the rate of 1% and NaCl at the concentration of 5% and incubated for six days under shaking conditions. The metabolites showed good antimicrobial activity against Gram positive and Gram negative bacteria, as well as unicellular and multicellular fungi. Conclusion: S. gulbargensis DAS 131 isolated from the semi-arid soils of Gulbarga, Northern Karnataka province, India exhibited broad spectrum antimicrobial activity. It was found that the antimicrobial metabolite production by the strain was positively influenced by carbohydrates, nitrogen sources and minerals.
References (33)
- 1 Marine natural products [DOI]
- 2 Bioactive Microbial Metabolites [DOI]
- 3 Selection of Media for Isolation of Streptomycetes [DOI]
- 4 Caboxamycin, a new antibiotic of the benzoxazole family produced by the deep-sea strain Streptomyces sp. NTK 937 [DOI]
- 5 Response surface methodological approach to optimize the nutritional parameters for neomycin production by Streptomyces marinensis under solid-state fermentation [DOI]
- 6 Factors affecting antimicrobial activity of Synechococcus leopoliensis [DOI]
- 7 Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces sp. 201 [DOI]
- 8 Optimization of process parameters for cephalosporin C production under solid state fermentation from Acremonium chrysogenum [DOI]
- 9 Pyrocoll, an Antibiotic, Antiparasitic and Antitumor Compound Produced by a Novel Alkaliphilic Streptomyces Strain [DOI]
- 10 Screening microbial metabolites for new drugs. Theoretical and practical issues. [DOI]
- 11 Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D [DOI]
- 12 Purification and Some Properties of a Keratinolytic Enzyme from an AlkaliphilicNocardiopsissp. TOA-1 [DOI]
- 13 Optimal Conditions for Antimicrobial Metabolites Production from a NewStreptomycessp. RUPA-08PR Isolated from Bangladeshi Soil [DOI]
- 14 Optimization of the cultivation medium for natamycin production byStreptomyces natalensis [DOI]
- 15 Utilization of carbon and nitrogen sources by Streptomyces kanamyceticus M 27 for the production of an Anti bacterial antibiotic [DOI]
- 16 Therapeutic treasures from the deep [DOI]
- 17 Optimization of Culturing Conditions for Improved Production of Bioactive Metabolites by Pseudonocardia sp. VUK-10 [DOI]
- 18 Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp., KGG32.
- 19 Optimisation of physical factors on the production of active metabolites by Bacillus subtilis 355 against wood surface contaminant fungi [DOI]
- 20 Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization [DOI]
- 21 Polyene Antibiotics from Streptomyces mediocidicus [DOI]
- 22 Optimization of nutritional requirements for gentamicin production by Micromonospora echinospora.
- 23 Effects of Minerals on Neomycin Production by Streptomyces fradiae [DOI]
- 24 Optimization of Fermentation Conditions for Antibiotic Production by Actinomycetes YJ1 Strain against Sclerotinia sclerotiorum [DOI]
- 25 Streptomyces gulbargensis sp. nov., isolated from soil in Karnataka, India [DOI]
- 26 Nutritional requirements for the production of pyrazoloisoquinolinone antibiotics by Streptomyces griseocarneus NCIMB 40447 [DOI]
- 27 Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain [DOI]
- 28 Isolation and Characterization of Antibiotic NC0604, a New Analogue of Bleomycin [DOI]
- 29 Biotechnological Application for Producing Some Antimicrobial Agents by Actinomycetes Isolates from Al-khurmah Governorate.
- 30 Mineral Nutrition of Streptomyces kanamyceticus for Kanamycin Formation [DOI]
- 31 Optimization Of Potential Antibiotic Production By Salt - Tolerant Actinomycetes Streptomyces SP. - MSU29 Isolated From Marine Sponge [DOI]
- 32 Physico-chemical characteristics of vernamycin-A antibiotic biosynthesis by streptomyces SP-AZ-SH-29 [DOI]
- 33 Biotechnological significance of Actinobacterial research in India [DOI]
Cited by 5
Elmutasim O. Ibnouf · Saudi Journal of Biological Sciences · 2021
Krishnan Raguvaran, Marissa Angelina, Manickam Kalpana · Process Safety and Environmental Protection · 2025
Showing 2 of 5 known citations — external sources report more than can currently be individually listed.
Related research
- Evaluation of Phytochemical Constituents and Antimicrobial Activity of Leaves and Stem Bark Extracts of Sarcocephalus latifolius — shares topic coverage
- Diversity and Antimicrobial Activity of Hydrobionts Associated Microorganisms from the Sea of Japan with the Occurrence of Tropodithietic Acid Producing Bacteria — shares topic coverage
- Antimicrobial Activity of Emilia pratermissa Leaf Extracts on Organisms Isolated from Patients with Otitis Media Attending Federal Medical Centre Owo and Ondo States Specialist Hospital Akure — shares topic coverage
- Molecular Identification, Bioactivity Screening and Metabolic Fingerprinting of the Actinomycetes of Chenab River Sediments — shares topic coverage
- Evaluating Efficacy of Biosurfactants from Bacterial Isolates in Conferring Protection against Rhizoctonia and Sclerotium Infection in Wheat and Peanut Plants — shares topic coverage
Article metrics
Real usage data collected on this platform.
48
Page views
0
PDF downloads
4
Outbound clicks
5
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.