Platelet-rich Fibrin Formation was delayed in Plastic Tubes
Budsara Jianpeampoolpol, Saranya Phuminart, Keskanya Subbalekha
Journal of Advances in Medicine and Medical Research · pp. 1–9 · Published 24 Mar 2016
10.9734/BJMMR/2016/25169Abstract
Introduction: Platelet-rich fibrin (PRF) is used in many regenerative treatments. Preparing PRF in glass tubes requires quick handling and generates biohazard concerns about silica contamination and glass breakage. Using plastic tubes may be an alternative to glass tubes. Objectives: This study investigated the formation of PRF prepared in polypropylene (PP) and polystyrene (PS) tubes compared with glass tubes. Methodology: PRF was prepared from human blood (n=20) in PP, PS, and glass tubes. The time required for PRF clot formation and retraction from the tube wall were observed. The PDGF-AB, TGF-β1 levels, and the gross/SEM appearance of the PRF clots were also evaluated. Results: The PRF clots in PP and PS tubes formed and retracted significantly slower compared with those in glass tubes. The PDGF-AB levels in the PRF from PP, PS, and glass tubes were not significantly different. Although the TGF-β1 levels in the PRF from PP and glass tubes were not different, that from the PRF from PS tubes was significantly higher. The gross structure and SEM appearance of PRF from the three tube types were similar. Conclusion: The slow clot formation in PP and PS tubes can extend PRF handling time while retaining PDGF-AB level and fibrin appearance. Owing to this delay in clot formation, we could obtain the plasma without adding any anticoagulant chemical agents. The plasma can be used for accelerating bone regeneration as platelet-rich plasma.
References (19)
- 1 Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF) [DOI]
- 2 Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution [DOI]
- 3 Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features [DOI]
- 4 Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift [DOI]
- 5 Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies [DOI]
- 6 Three‐Dimensional Architecture and Cell Composition of a Choukroun's Platelet‐Rich Fibrin Clot and Membrane [DOI]
- 7 Sinus Floor Augmentation With Simultaneous Implant Placement Using Choukroun's Platelet‐Rich Fibrin as the Sole Grafting Material: A Radiologic and Histologic Study at 6 Months [DOI]
- 8 Hematology: principles and procedures
- 9 Autologous Platelet‐Rich Fibrin in the Treatment of Mandibular Degree II Furcation Defects: A Randomized Clinical Trial [DOI]
- 10 Treatment of 3‐Wall Intrabony Defects in Patients With Chronic Periodontitis With Autologous Platelet‐Rich Fibrin: A Randomized Controlled Clinical Trial [DOI]
- 11 In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF [DOI]
- 12 Safety Issues Associated With Platelet-Rich Fibrin Method [DOI]
- 13 Polymer Chemistry: Properties and Application
- 14 Dental And Craniofacial Applications Of Platelet-Rich Plasma
- 15 Distinctions and Correspondences among Surfaces Contacting Blood [DOI]
- 16 A new plastic evacuated tube with plasma separator [DOI]
- 17 Plastic containers and the whole-blood clotting test: glass remains the best option [DOI]
- 18 Platelet adhesion to surfaces [DOI]
- 19 Blood-Contacting Polymers [DOI]
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