Potential of Azadirachtin and Neem (Azadirachta indica) Based Saponins as Biopesticides for In vitro Insect Pests Cellulase (Beta-1,4-Endoglucanase) Enzyme Inhibition and In vivo Repellency on Tribolium castaneum
Amtul Jamil Sami, A. R. Shakoori
Biotechnology Journal International · pp. 904–917 · Published 6 Aug 2014
10.9734/BBJ/2014/6903Abstract
Aims: The work was undertaken to identify the role of Neem derived compounds (saponins and azadirachtin) on the digestive cellulose hydrolyzing enzyme activity of red flour beetle (T. castaneum), rice grasshopper (Oxya chinensis) and red pumpkin beetle (Aulacophora foveicolis). Place and Duration of Study: The work was carried out at the Institute of Biochemistry and Biotechnology University of the Punjab Lahore Pakistan. Methodology: Total cellular proteins were isolated from the insect’s gut and salivary glands and were tested for cellulose hydrolyzing activity on substrate agar plates. Saponins and Azadirachtin were isolated from Azadirachta indica tissues and used for enzyme inhibition studies. Repellancy test was performed for T. Castaneum, using saponins and Azadirachtin. For computational studies sequence of endoglucanase gene was identified from T. castaneum genome and protein structure was deduced. Results: Saponins were able to inhibit beta-1,4-endoglucanase enzyme activity, present in all the three insect pests. A computational dissection of T. castaneum endoglucanase enzyme, deduced from T. castaneum genome, showed that there were five cys involved in the formation of disulphide bridges in the molecule. The disulfide bridges did not provide any protection to endoglucanase active site. Azadirachtin had no effect on cellulase activity of Oxya chinensis and Aulacophora foveicolis, while beta-1,4-endoglucanase activity of T. castaneum was inhibited. Repellency test for T. castaneum revealed that each group of compounds (Saponins and Azadirachtin) was able to repel the insect. Conclusion: Neem derived compounds had a considerable inhibitory effect on the digestive cellulose hydrolyzing enzyme of T. castaneum.
References (22)
- 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
- 2 BOTANICAL INSECTICIDES, DETERRENTS, AND REPELLENTS IN MODERN AGRICULTURE AND AN INCREASINGLY REGULATED WORLD [DOI]
- 3 The biological action of saponins in animal systems: a review [DOI]
- 4 Azadirachtin from the neem tree Azadirachta indica: its action against insects [DOI]
- 5 Insecticidal and antifeedant bioactivities of neem oils and their relationship to azadirachtin content [DOI]
- 6 Inhibition of cellulases by phenols [DOI]
- 7 Neem—An Omnipotent Plant: A Retrospection [DOI]
- 8 Properties And Potential Of Natural Pesticides From The Neem Tree, Azadirachta Indica [DOI]
- 9 Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes [DOI]
- 10 Effect of neem limonoids on lactate dehydrogenase (LDH) of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) (Insecta: Lepidoptera: Pyralidae) [DOI]
- 11 Monograph on neem (Azadirachta indica A. Juss.).
- 12 Investigation of the locust feeding inhibition of the seeds of the neem tree, Azadirachta indica [DOI]
- 13 Extraction and Colorimetric Determination of Azadirachtin-Related Limonoids in Neem Seed Kernel [DOI]
- 14 Limonoids from the seeds of Azadirachta indica [DOI]
- 15 Identification, cloning, and expression of a GHF9 cellulase from Tribolium castaneum (Coleoptera: Tenebrionidae) [DOI]
- 16 Structure-related insect antifeedant and growth regulating activities of some limonoids [DOI]
- 17 Identification of a Novel Triterpenoid Saponin from Pisum sativum as a Specific Inhibitor of the Diguanylate Cyclase of Acetobacter xylinum [DOI]
- 18 Tricyclic diterpenoids from root bark of Azadirachta indica [DOI]
- 19 Inhibition of turnover of neurosecretion by azadirachtin in Locusta migratoria [DOI]
- 20 Isolation of a triterpenoid from Azadirachta indica [DOI]
- 21 Larvicidal activity of the saponin fractions of Chlorophytum borivilianum santapau and Fernandes [DOI]
- 22 Natural products: the secondary metabolites.
Cited by 20
L. Li, X. Song, Z. Yin · Arquivo Brasileiro de Medicina Veterinária e Zootecnia · 2019
Esra Koç, Belgizar Karayiğit · Climate-Resilient Agriculture, Vol 1 · 2023
Mervat Abdel-Moneauim Mostafa El-Genaidy, Mohamed Abd El-Aziz Mohamed Hindy, Nehad Abdel-Hameed Soliman · Polish Journal of Entomology · 2021
Qudsia Kanwal, Aneela Anwar, Samina Akbar · Biocatalysis and Agricultural Biotechnology · 2017
Miftachur Rohma, Moh Mirza Nuryady, Sri Wahyuni · Jurnal Ilmu-Ilmu Pertanian Indonesia · 2021
Hasna Nisrina Sumayyah, Widya Rahmanisa Fitri, Tania Surya Utami · AIP Conference Proceedings · 2024
Soma Prasad Sahoo, Bighneswar Baliyarsingh · 2024
Irys Fernanda Santana Couto, Silvana Aparecida Souza, Fabricio Iglesias Valente · Gesunde Pflanzen · 2020
Asma Mami Maazoun, Soumaya Haouel Hamdi, Feten Belhadj · Environmental Science and Pollution Research · 2019
Sai Snehitha Yadavalli, Bratati Sen, Swati Ghosh Acharyya · IEEE Sensors Journal · 2023
Showing 11 of 20 known citations — external sources report more than can currently be individually listed.
Related research
- Phytochemical and Antibacterial Investigation of Leaf Extracts of Vernonia amygdalina — shares topic coverage
- Utilization of Saponins, a Plant Secondary Metabolite in Enteric Methane Mitigation and Rumen Modulation — shares topic coverage
- Saponins and Polyphenolics of Methanol Leaf Extract of Boswellia dalzielii Hutch — shares topic coverage
- Evaluation of Haemolytic Activity of Leaves from Acacia podalyriifolia — shares topic coverage
- Synergistic Antimicrobial and Antioxidant Activity of Saponins-Rich Extracts from Paronychia argentea and Spergularia marginata — shares topic coverage
Article metrics
Real usage data collected on this platform.
69
Page views
0
PDF downloads
8
Outbound clicks
20
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.