Skip to content
Research Article Open access CC BY 4.0

Preliminary Metabolomics and Determination of a Potent Anti-inflammatory Fraction of Freshwater Brown Algae

Nwankwo Valerie Ezinne, Muoegbunam Juliet, Abba Chika, Okoye Festus B.C, Ugwu Chukwuebuka C, Ngwoke Kenneth G

Asian Plant Research Journal · pp. 56–68 · Published 24 Jan 2025

10.9734/aprj/2025/v13i1293

Abstract

Objective: The study aims to perform preliminary metabolomics and identify the potent anti-inflammatory fractions from freshwater brown algae extract. Materials and Methods: The brown algae sample was harvested from the exposed rocks in  Ebonyi River in Nigeria, and was subjected to cold maceration in a methanol-dichloromethane (2:1) solvent mixture for 72 hours, for extraction. The resulting extract was concentrated and subsequently fractionated via vacuum liquid chromatography (VLC) using solvent gradients of n-hexane and ethyl acetate. Acute toxicity was evaluated in Swiss albino mice according to Lorke’s method, with mortality rates employed to determine the LD50. The anti-inflammatory potential of the fractions was assessed using both in vitro (protein denaturation and heat-induced hemolysis assays) and in vivo (acetic acid-induced vascular permeability and xylene-induced edema) models. Doses of 50 and 100 mg/kg as well as 1 and 2 mg of the fractions (3:7, 5:5, 7:3) were administered orally and topical for the in vivo models respectively, while doses ranging from 62.5 to 1000 μg were used for the in vitro test. Dexamethasone was used as the positive control. High-performance liquid chromatography (HPLC) dereplication was employed to identify the phytoconstituents of the fractions. Results: Many anti-inflammatory compounds, including quercetin, ellagic acid and Kaempferol were identified in the fractions which would have accounted for the pronounced anti-inflammatory properties observed. Fraction 5:5 showed a superior anti-inflammation compared to dexamethasone in the xylene-induced edema model, although not statistically significantly different. Conclusion: These fractions, especially 5:5 can be further developed for the treatment of inflammation.

Anti-inflammatory Nigeria rivers animals methanol

References (16)

  1. 1 Inflammatory responses and inflammation-associated diseases in organs [DOI]
  2. 2 The cytokine storm and COVID‐19 [DOI]
  3. 3 Recent Advances in Potential Health Benefits of Quercetin [DOI]
  4. 4 Inflammation-Induced Tumorigenesis and Metastasis [DOI]
  5. 5 Multifunctional role of fucoidan, sulfated polysaccharides in human health and disease: A journey under the sea in pursuit of potent therapeutic agents [DOI]
  6. 6 Brown Algae as Functional Food Source of Fucoxanthin: A Review [DOI]
  7. 7 Antibacterial, Anti‐Inflammatory, Antioxidant, and Antiproliferative Properties of Essential Oils from Hairy and Normal Roots of Leonurus sibiricus L. and Their Chemical Composition [DOI]
  8. 8 Chemistry of Protein-Phenolic Interactions Toward the Microbiota and Microbial Infections [DOI]
  9. 9 Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro [DOI]
  10. 10 Research Progress in Anti-Inflammatory Bioactive Substances Derived from Marine Microorganisms, Sponges, Algae, and Corals [DOI]
  11. 11 Studies on anti-inflammatory and analgesic activities of betel nut in rodents [DOI]
  12. 12 Metabolites from Combretum dolichopetalum and its associated endophytic fungus Nigrospora oryzae — Evidence for a metabolic partnership [DOI]
  13. 13 The Fungal Endophyte Nigrospora oryzae Produces Quercetin Monoglycosides Previously Known Only from Plants [DOI]
  14. 14 Flavonoid glycosides from Olax mannii: Structure elucidation and effect on the nuclear factor kappa B pathway [DOI]
  15. 15 Curcumin Confers Anti-Inflammatory Effects in Adults Who Recovered from COVID-19 and Were Subsequently Vaccinated: A Randomized Controlled Trial [DOI]
  16. 16 In vitro Anti-oxidant Activity and HPLC-DAD System Based Phenolic Content Analysis of Codiaeum variegatum Found in Bangladesh [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

20

Page views

0

PDF downloads

1

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.