Skip to content
Research Article Open access CC BY 4.0

Prevalence of Entamoeba histolytica Infection Using Microscopy and Adhesin Detection Methods among School Children in Central Nigeria

Oti B. Victor, Galleh P. Raphael, Ezhim I. Moses, Tsaku A. Paul, Ajegena S. Abimiku, Oti C. Augustina, Oti B. Isaac

Asian Journal of Biology · pp. 1–9 · Published 22 Jun 2017

10.9734/AJOB/2017/34291

Abstract

This study aimed to evaluate the prevalence of Entamoeba histolytica infection using microscopy and adhesin detection methods among school children in Central Nigeria. After ethical clearance, stool samples of three hundred and sixty (360) children aged 5-16 years old as adopted by FMOH (2013) were randomly collected between September-December 2015 and were examined for E. histolytica cysts/trophozoites using microscopy and TechLab E. histolytica II ELISA test (Sigma Diagnostic Inc, USA)  in six public primary schools in Keffi, Nasarawa State. The TechLab E. histolytica II ELISA result showed that out of the 88 (24.4%) stool samples positive by microscopy, 85 (96.6%) samples were positive for E. histolytica antigens while 3 (3.4%) were negative. The overall prevalence of the infection using microscopy method was 24.4%. Males recorded a higher prevalence 64 (30.5%) than females 24 (16.0%). Children aged 6-10 years recorded the highest prevalence of 37 (28.5%) while the lowest 22 (21.6%) was showed in those aged <6 years. There was significant difference in the infection rate among the sex of the children (p≤ 0.05). The association was also significant based on location of the children’s homes and finger sucking habit. Children living outside Keffi town had a higher prevalence 34 (25.4%) than those living in Keffi town 54 (23.9%). Out of the children infected, 23 (32.9%) suck their finger while 65 (22.4%) did not. In this study age, pupil’s class, occupations of caregivers, sources of drinking water and types of toilet facility had no statistical significance with E. histolytica infection (p 0.05). Health education programmes that will promote personal and environmental hygiene and regular mass treatment are suggested strategies to mitigate transmission of the infection especially in children who are usually asymptomatic.

Prevalence Entamoeba histolytica microscopy ELISA Keffi

References (17)

  1. 1 District Laboratory Practice in Tropical Countries [DOI]
  2. 2 Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar [DOI]
  3. 3 Geographic Diversity among Genotypes of Entamoeba histolytica Field Isolates [DOI]
  4. 4 Prevalence and characters of Entamoeba histolytica infection in Saudi infants and children admitted with diarrhea at 2 main hospitals at south Jeddah: a re-emerging serious infection with unusual presentation [DOI]
  5. 5 Prevalence and Spatial Distribution of Entamoeba histolytica/dispar and Giardia lamblia among Schoolchildren in Agboville Area (Côte d'Ivoire) [DOI]
  6. 6 PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy [DOI]
  7. 7 Demographic status and prevalence of intestinal parasitic infections in schoolchildren in Izmir, Turkey [DOI]
  8. 8 diagnosis and differentiation of entamoeba infection in makkah al mukarramah using microscopy and stool antigen detection kitas
  9. 9 Prevalence of intestinal parasites among primary school children in Makurdi, Benue State- Nigeria [DOI]
  10. 10 Determination of the prevalence of Entamoeba histolytica in human at a private fertilizer company hospital in Pakistan using microscopic technique
  11. 11 Prevalence of Entamoeba histolytica among primary school children in Akure, Ondo State, Nigeria [DOI]
  12. 12 Frequency of Entamoeba histolytica and Entamoeba dispar prevalence among patients with gastrointestinal complaints in Chelgerd city, southwest of Iran(*).
  13. 13 Prevalence of Intestinal Amoebiasis in Infant and Junior School Children in Degema General Hospital and Environs [DOI]
  14. 14 Entamoeba histolytica Infection in Children Aged 0-12 Years in Rural Communities in Anambra State, Nigeria
  15. 15 Seroprevalence of Herpes Simplex Virus Type 2 (HSV-2) Infection Among HIV Patients Accessing Healthcare at Federal Medical Centre, Keffi, Nigeria [DOI]
  16. 16 Evaluation of Entamoeba Histolytica Transmission Rates amongst Primary School Children in Five Selected Communities in Gwagwalada Area Council, Fct, Abuja, Nigeria
  17. 17 Journal of Public Health and Epidemiology [DOI]

Cited by 6

Antidiabetic Properties of Aqueous Leaf and Stem Extracts of Andrographis paniculata on Alloxan Induced Diabetes in Wistar Rats

Yusuf, Z. H., Dan, V. M. Y., Magaji, Y. · UMYU Journal of Microbiology Research (UJMR) · 2022

ETIOLOGY OF BLOODY DIARRHEA IN CHILDREN AND PREVALENCE OF E. COLI O157:H7

Twana Kareem, Sherko Omer · JOURNAL OF SULAIMANI MEDICAL COLLEGE · 2020

Showing 2 of 6 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

38

Page views

0

PDF downloads

3

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.