Skip to content
Research Article Open access CC BY 4.0

Probiotic Bacteria in Gastro-Intestinal Tract of Hybrid Catfish Fed Insect Protein Meal

Aliyu-Paiko Mohammed, Kate Isioma Iloba, Aliyat Adetayo Adediran, Yakubu Manbe Mohammed, Maureen Chukwu, Kabir Mohammed Adamu

Asian Journal of Fisheries and Aquatic Research · pp. 1–13 · Published 25 Jan 2023

10.9734/ajfar/2023/v21i2529

Abstract

The evaluations of insects’ protein as viable alternative protein source in fish diets are gaining more attention revealing that the protein has more comparative advantage than other protein sources. Therefore, this study was conducted to isolate and identify (using phylotypic and genotypic methods) bacteria isolates in the gastrointestinal tract (GIT) of African hybrid catfish fed supplemented grasshopper meal. Four feeding diets were adopted in this study; Diet A (conventional feed), Diet B (100% Grasshopper meal), Diet C (50% fishmeal and 50% Grasshopper meal) and Diet D (100% Fish meal). At the end of the 12- week experimental period, the fish were sacrificed for their GIT contents to determine bacteria diversity. In the catfish fed diet A, the bacteria isolates identified were Streptococcus pyogenes, Enterobacter aerogene, Staphylococcus aureus, Shigella flexneri, Escherichia coli, Leuconostoc latis and Klebsella pneumonia. In catfish fed diet B, Bacillus subtilis, Lactococcus bulgaricus, Lysinibacillus macroides, Lysinibacillus capsici, Stenotrophomonas pavanii and Lysinibacillus sp were isolated. Similarly, the fish fed diet C recorded the presence of Bacillus subtilis, Lactococcus bulgaricus, Lysinibacillus sp., Staphylococcus aureus, Lysinibacillus macroides, Stenotrophomonas pavanii and Enterobacter aerogene. The bacteria isolated in the GIT of fish fed diet D were Bacillus cereus, Staphylococcus aureus, Pedicoccus acidilactis, Escherichia coli, Salmonella typhi, Leuconostoc latis and Shigella flexneri.  Probiotic bacteria were more in fish fed with diets B and C, an indication that the different ratio of supplemented grasshopper meal is good for the fish, as they have the potentials to boost the fish immune system and improve the microbial balance of its gastro-intestinal tract.

Bacteria freshwater fish grasshopper nutrition

References (27)

  1. 1 The neighbor-joining method: a new method for reconstructing phylogenetic trees. [DOI]
  2. 2 MEGA11: Molecular Evolutionary Genetics Analysis Version 11 [DOI]
  3. 3 Medical Laboratory Manual for Tropical Countries
  4. 4 Role of gastrointestinal microbiota in fish [DOI]
  5. 5 The State of World Fisheries and Aquaculture 2016 [DOI]
  6. 6 Isolation and characterization of native probiotics for fish farming [DOI]
  7. 7 Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens [DOI]
  8. 8 Isolation and Partial Characterization of Lactic Acid Bacteria from the Gut Microbiota of Marine Fishes for Potential Application as Probiotics in Aquaculture [DOI]
  9. 9 Gnotobiological Study of Infective Juveniles and Symbionts of Steinernema scapterisci: A Model to Clarify the Concept of the Natural Occurrence of Monoxenic Associations in Entomopathogenic Nematodes [DOI]
  10. 10 Quantitative analyses of the bacterial microbiota of rearing environment, tilapia and common carp cultured in earthen ponds and inhibitory activity of its lactic acid bacteria on fish spoilage and pathogenic bacteria [DOI]
  11. 11 The use of (GTG)5 oligonucleotide as an RAPD primer to type Campylobacter concisus [DOI]
  12. 12 Evaluation of nutrient quality of a short horned grasshopper, Oxya hyla hyla Serville (Orthoptera: Acrididae) in search of new protein source
  13. 13 Bacterial Flora of African Catfish (Clarias gariepinus ) Harvested From Ponds In Uyo South-South Nigeria [DOI]
  14. 14 Host-derived Probiotics for Finfish Aquaculture [DOI]
  15. 15 Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties [DOI]
  16. 16 Effects of Grasshopper Meal in the Diet of Clarias Gariepinus Fingerlings [DOI]
  17. 17 Molecular Characterization of Bacteria Isolates from Farm-Raised Catfish Clarias gariepinus (Burchell, 1822) [DOI]
  18. 18 ENVIRONMENTAL FRIENDLY AQUACULTURE KEY TO SUSTAINABLE FISH FARMING DEVELOPMENT IN NIGERIA [DOI]
  19. 19 Nutritional evaluation of a short-horned grasshopper‚ Oxya hyla hyla (Serville) meal as a substitute of fishmeal in the compound diets of rohu, Labeo rohita (Hamilton) [DOI]
  20. 20 A Preliminary Study on Oxya fuscovittata (Marschall) as an Alternative Nutrient Supplement in the Diets of Poecillia sphenops (Valenciennes) [DOI]
  21. 21 Bacterial Diversity in the Digestive Tracts of Four Indian Air-Breathing Fish Species Investigated by PCR Based Denaturing Gradient Gel Electrophoresis [DOI]
  22. 22 Carcass Proximate Composition and Amino acid Profiles of Hybrid Catfish fed Supplementary Cockroach Meal [DOI]
  23. 23 Bacteria and fungi analyses of fish diets with grasshopper and cockroach meals: the potential replacement of fishmeal in fish diets [DOI]
  24. 24 Microbial flora of the gastro-intestinal tract of Clarias gariepinus caught from river Dandaru Ibadan, Nigeria [DOI]
  25. 25 Bacterial Flora of Wild and Cultured Clariasgariepinus (African Catfish) and their Public Health Implications. [DOI]
  26. 26 Iranian Journal of Fisheries Sciences [DOI]
  27. 27 Nigerian Journal of Fisheries and Aquaculture [DOI]

Cited by 1

Dietary supplement using cockroach meal: impact on growth indices and biochemical responses in hybrid catfish

Abiola Olabisi Kolawole, Kabir Mohammed Adamu, Ibrahim Baba Usman · EUREKA Life Sciences · 2023

Related research

Article metrics

Real usage data collected on this platform.

55

Page views

0

PDF downloads

15

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.