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Research Article Open access CC BY 3.0

Quantitative Estimation of Piperine and Diosgenin in Ayurvedic Formulation

Sandhya Parameswaran, Shalaka Koshti

Journal of Pharmaceutical Research International · pp. 910–919 · Published 22 Feb 2014

10.9734/BJPR/2014/8310

Abstract

Goksuradi guggulu is a polyherbal formulation official in “Ayurvedic formulary of India” and used for dysuria, urinary obstruction, increased frequency and turbidity of urine, calculus, excessive vaginal discharge, gout. A simple, specific and precise high performance thin-layer chromatography (HPTLC) method has been developed for quantification of piperine and diosgenin in Goksuradi guggulu. We report the extraction and estimation of these compounds in a laboratory prepared sample of Goksuradi guggulu and two of its marketed formulations. The compounds were chromatographed on precoated silica gel G 60 254 plates in the mobile phase comprising of toluene: hexane: ethyl acetate (6.8:0.2:3). Under the optimized chromatographic conditions, the calibration plot was found to be linear in the range of 0.2-1µg spot-1 for piperine and 1.0 -3.0 µg spot-1 for diosgenin. The correlation coefficient (R2) was 0.9979 and 0.9915 for piperine and diosgenin respectively. Mean recovery (% w/w) for piperine in all the formulations was in the range of 97.61 - 98.90 and for diosgenin, it was 93.76 - 94.33.

Piperine diosgenin HPTLC quantitation

References (4)

  1. 1 Anti-thrombosis effect of diosgenin extract from Dioscorea zingiberensis C.H. Wright in vitro and in vivo [DOI]
  2. 2 Isolation of piperine from black pepper [DOI]
  3. 3 A Validated Quantitative Thin-Layer Chromatographic Method for Estimation of Diosgenin in Various Plant Samples, Extract, and Market Formulation [DOI]
  4. 4 HPTLC Method Development and Validation for the simultaneous Estimation of Diosgenin and Levodopa in marketed formulation

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