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Research Article Open access CC BY 3.0

Rapid Oral Fluid HIV Antibody Testing in Exposed Babies: A Diagnostic Study

Regina Oladokun, Nkiru David, Biobele Brown, Kikelomo Osinusi

International Journal of TROPICAL DISEASE & Health · pp. 530–539 · Published 25 Feb 2014

10.9734/IJTDH/2014/7761

Abstract

Aims: The usefulness of rapid oral fluid HIV antibody tests has rarely been evaluated in exposed babies. Study Design: A diagnostic survey comparing the performance of oral fluid HIV antibody test and the routine rapid blood screening test. Place and Duration of Study: University College Hospital, Ibadan and Nigerian Institute of Medical Research, Lagos, between May 2010 and April 2011. Methodology: The study involved children aged less than 18 months referred for screening in two large HIV care programmes in Nigeria using rapid antibody tests - an oral fluid test (Test A) and the routine blood test (Test B). The testing was blinded and HIV status was confirmed using DNA PCR. Results: A total of 94 children were studied with ages ranging from 0.13 to less than 18months. Out of the 94 parallel tests, when compared with DNA PCR, there were 7 (7.5%) discordant results. Test A gave one false positive, one false negative and no indeterminate result. Test B gave four false positive, one false negative and two indeterminate results. Test A had a sensitivity of 93.3%, specificity of 98.7%, positive predictive value of 93.3% and negative predictive value of 98.7% compared with Test B which had 90.0%, 92.9%, 60.0% and 98.7% respectively. Among the caregivers 88 (93.6%) preferred oral fluid testing to blood as it is painless and easy to perform. Conclusion: Compared with the rapid antibody blood test, the oral fluid test correlates better with DNA PCR in detecting the absence of infection in HIV exposed babies. Given this performance, it may be useful in expanding testing in HIV exposed children in settings where there are challenges with early infant diagnosis.

Early Infant Diagnosis HIV antibody Rapid test Oral fluid

References (18)

  1. 1 Early infant HIV-1 diagnosis programs in resource-limited settings: opportunities for improved outcomes and more cost-effective interventions [DOI]
  2. 2 Diagnosis of HIV-1 Infection in Children Younger Than 18 Months in the United States [DOI]
  3. 3 Evaluation and Implementation of Rapid HIV Tests: The Experience in 11 African Countries [DOI]
  4. 4 Evaluation of Diagnostic Accuracy, Feasibility and Client Preference for Rapid Oral Fluid-Based Diagnosis of HIV Infection in Rural India [DOI]
  5. 5 Validation of Rapid HIV Antibody Tests in 5 African Countries [DOI]
  6. 6 Cost-Effectiveness of Routine Rapid Human Immunodeficiency Virus Antibody Testing Before DNA-PCR Testing for Early Diagnosis of Infants in Resource-Limited Settings [DOI]
  7. 7 Serological diagnosis of HIV infection using oral fluid samples.
  8. 8 Predicting perinatal human immunodeficiency virus infection by antibody patterns [DOI]
  9. 9 Investigation of False Positive Results with an Oral Fluid Rapid HIV-1/2 Antibody Test [DOI]
  10. 10 Evaluation of Youth Preferences for Rapid and Innovative Human Immunodeficiency Virus Antibody Tests [DOI]
  11. 11 Evaluation of a measles vaccine campaign by oral-fluid surveys in a rural Kenyan district: interpretation of antibody prevalence data using mixture models [DOI]
  12. 12 An evaluation of the performance of OraQuick® ADVANCE Rapid HIV-1/2 Test in a high-risk population attending genitourinary medicine clinics in East London, UK [DOI]
  13. 13 Evaluation of diagnostic tests for HIV infection in infants born to HIV-infected mothers in Switzerland [DOI]
  14. 14 Can Oral Fluid Testing Be Used to Replace Blood-Based HIV Rapid Testing to Improve Access to Diagnosis in South Africa? [DOI]
  15. 15 Towards universal access: scaling up HIv services for women and children in the health sector: progress report 2008
  16. 16 Saliva‐Based HIV Testing among Secondary School Students in Tanzania using the OraQuick® Rapid HIV1/2 Antibody Assay [DOI]
  17. 17 IgG, IgM, and IgA response to HIV in infants born to HIV-1 infected mothers. Swiss Neonatal HIV Study Group.
  18. 18 Revised Recommendations for HIV Testing of Adults, Adolescents, and Pregnant Women in Health-Care Settings [DOI]

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